A mitogen- and anisomycin-stimulated kinase phosphorylates HMG-14 in its basic amino-terminal domain in vivo and on isolated mononucleosomes

M. J. Barratt, C. A. Hazzalin, N. Zhelev, L. C. Mahadevan*

*Corresponding author for this work

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The rapid, transient induction of 80-100 immediate-early (IE) genes upon mitogenic stimulation occurs irrespective of protein synthesis and is mediated by modification of existing proteins. Two mechanisms, not mutually exclusive, involving modification either of sequence-specific transcription factors or of structural chromatin proteins primed by pre-association with responsive effectors are conceivable. Here, we show that upon IE gene induction, the non-histone high-mobility-group protein HMG-14, but not the related protein HMG-17, becomes serine phosphorylated in its basic, amino-terminal region close to where it binds nucleosomal DNA. Phosphorylation, normally transient, occurs independent of transcription and is quantitative and prolonged during superinduction. Brief micrococcal nuclease digestion substantially releases HMG-14 from nuclei in the mononucleosome-bound state. Finally, mononucleosomes prepared from mitogen-stimulated, but not control, cells contain a mitogen-activated kinase that phosphorylates HMG-14 in vitro on the same site(s) as in intact cells. The association of HMG-14 and its mitogen-activated kinase with nuclease-sensitive mononucleosomes has implications for models of mitogen-stimulated IE gene induction.

Original languageEnglish
Pages (from-to)4524-4535
Number of pages12
JournalEMBO Journal
Volume13
Issue number19
DOIs
Publication statusPublished - 25 Oct 1994
Externally publishedYes

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HMGN1 Protein
Anisomycin
Mitogens
Immediate-Early Genes
Phosphotransferases
Genes
HMGN2 Protein
Association reactions
High Mobility Group Proteins
Micrococcal Nuclease
Phosphorylation
Proteins
Transcription
Serine
Chromatin
Digestion
Transcription Factors
DNA

Cite this

Barratt, M. J. ; Hazzalin, C. A. ; Zhelev, N. ; Mahadevan, L. C. / A mitogen- and anisomycin-stimulated kinase phosphorylates HMG-14 in its basic amino-terminal domain in vivo and on isolated mononucleosomes. In: EMBO Journal. 1994 ; Vol. 13, No. 19. pp. 4524-4535.
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A mitogen- and anisomycin-stimulated kinase phosphorylates HMG-14 in its basic amino-terminal domain in vivo and on isolated mononucleosomes. / Barratt, M. J.; Hazzalin, C. A.; Zhelev, N.; Mahadevan, L. C.

In: EMBO Journal, Vol. 13, No. 19, 25.10.1994, p. 4524-4535.

Research output: Contribution to journalArticle

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