A novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1

Douglas Lester, Colin Farquharson, I R Paton, E Seawright, David Jefferies, C. C. Whitehead, D W Burt, Brian Houston

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The growth plate is a specialised region of cartilage located at the growing ends of long bones in higher vertebrates. It is responsible for longitudinal bone growth and is under the control of many local and systemic factors. The growth plate consists of an orderly arrangement of small proliferative and larger mature hypertrophic chondrocytes. This paper describes the isolation by differential display of a 988-bp cDNA fragment derived from a transcript that is more highly expressed in proliferating rather than hypertrophic chondrocytes of the chick growth plate. Using 3' RACE, a further 939 bp of cDNA sequence was obtained. The 1.9 kb sequence contains a 924-bp open reading frame encoding an unknown 308 amino acid protein. This protein has a putative transmembrane domain near its N-terminus and three dileucine motifs at its carboxy tail. This gene was expressed in all other tissues examined. A polymorphism was identified by SSCP analysis and the gene was mapped to the centromeric region of the short arm of chicken chromosome 1, close to the locus for autosomal dwarfism.

    Original languageEnglish
    Pages (from-to)300-303
    Number of pages4
    JournalAnimal Genetics
    Volume30
    Issue number4
    DOIs
    Publication statusPublished - Aug 1999

    Fingerprint

    growth plate
    Growth Plate
    Chromosomes, Human, Pair 1
    membrane proteins
    Membrane Proteins
    chicks
    chondrocytes
    Chondrocytes
    chromosomes
    Complementary DNA
    bones
    Dwarfism
    Single-Stranded Conformational Polymorphism
    dwarfing
    Bone Development
    cartilage
    Open Reading Frames
    Genes
    Cartilage
    open reading frames

    Cite this

    Lester, D., Farquharson, C., Paton, I. R., Seawright, E., Jefferies, D., Whitehead, C. C., ... Houston, B. (1999). A novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1. Animal Genetics, 30(4), 300-303. https://doi.org/10.1046/j.1365-2052.1999.00473.x
    Lester, Douglas ; Farquharson, Colin ; Paton, I R ; Seawright, E ; Jefferies, David ; Whitehead, C. C. ; Burt, D W ; Houston, Brian. / A novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1. In: Animal Genetics. 1999 ; Vol. 30, No. 4. pp. 300-303.
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    abstract = "The growth plate is a specialised region of cartilage located at the growing ends of long bones in higher vertebrates. It is responsible for longitudinal bone growth and is under the control of many local and systemic factors. The growth plate consists of an orderly arrangement of small proliferative and larger mature hypertrophic chondrocytes. This paper describes the isolation by differential display of a 988-bp cDNA fragment derived from a transcript that is more highly expressed in proliferating rather than hypertrophic chondrocytes of the chick growth plate. Using 3' RACE, a further 939 bp of cDNA sequence was obtained. The 1.9 kb sequence contains a 924-bp open reading frame encoding an unknown 308 amino acid protein. This protein has a putative transmembrane domain near its N-terminus and three dileucine motifs at its carboxy tail. This gene was expressed in all other tissues examined. A polymorphism was identified by SSCP analysis and the gene was mapped to the centromeric region of the short arm of chicken chromosome 1, close to the locus for autosomal dwarfism.",
    author = "Douglas Lester and Colin Farquharson and Paton, {I R} and E Seawright and David Jefferies and Whitehead, {C. C.} and Burt, {D W} and Brian Houston",
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    Lester, D, Farquharson, C, Paton, IR, Seawright, E, Jefferies, D, Whitehead, CC, Burt, DW & Houston, B 1999, 'A novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1', Animal Genetics, vol. 30, no. 4, pp. 300-303. https://doi.org/10.1046/j.1365-2052.1999.00473.x

    A novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1. / Lester, Douglas; Farquharson, Colin; Paton, I R; Seawright, E; Jefferies, David; Whitehead, C. C.; Burt, D W; Houston, Brian.

    In: Animal Genetics, Vol. 30, No. 4, 08.1999, p. 300-303.

    Research output: Contribution to journalArticle

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    AU - Lester, Douglas

    AU - Farquharson, Colin

    AU - Paton, I R

    AU - Seawright, E

    AU - Jefferies, David

    AU - Whitehead, C. C.

    AU - Burt, D W

    AU - Houston, Brian

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    AB - The growth plate is a specialised region of cartilage located at the growing ends of long bones in higher vertebrates. It is responsible for longitudinal bone growth and is under the control of many local and systemic factors. The growth plate consists of an orderly arrangement of small proliferative and larger mature hypertrophic chondrocytes. This paper describes the isolation by differential display of a 988-bp cDNA fragment derived from a transcript that is more highly expressed in proliferating rather than hypertrophic chondrocytes of the chick growth plate. Using 3' RACE, a further 939 bp of cDNA sequence was obtained. The 1.9 kb sequence contains a 924-bp open reading frame encoding an unknown 308 amino acid protein. This protein has a putative transmembrane domain near its N-terminus and three dileucine motifs at its carboxy tail. This gene was expressed in all other tissues examined. A polymorphism was identified by SSCP analysis and the gene was mapped to the centromeric region of the short arm of chicken chromosome 1, close to the locus for autosomal dwarfism.

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