A rapid colorimetric assay of killer toxin activity in yeast

Valerie J. Hodgson, Graeme M. Walker, David Button

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability.
Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalFEMS Microbiology Letters
Volume120
Issue number1-2
DOIs
Publication statusPublished - 1994

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