A rapid colorimetric assay of killer toxin activity in yeast

Valerie J. Hodgson, Graeme M. Walker, David Button

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability.
    Original languageEnglish
    Pages (from-to)201-205
    Number of pages5
    JournalFEMS Microbiology Letters
    Volume120
    Issue number1-2
    DOIs
    Publication statusPublished - 1994

    Keywords

    • MTT-colorimetric assay
    • Killer toxin
    • Williopsis mrakii
    • Candicidal activity

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