A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

M. Adilia Lemos, J. A. Teixeira, M. Mota, F. M. Gama

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.
Original languageEnglish
Pages (from-to)703-707
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number8
DOIs
Publication statusPublished - 2000

Fingerprint Dive into the research topics of 'A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease'. Together they form a unique fingerprint.

  • Cite this