A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

M. Adilia Lemos, J. A. Teixeira, M. Mota, F. M. Gama

Research output: Contribution to journal › Article

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Abstract

Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.
Original languageEnglish
Pages (from-to)703-707
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number8
DOIs
StatePublished - 2000

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Cellulases
Cellulose
Digestion
Peptide Hydrolases
Trichoderma
Papain
Ultrafiltration
Capillary Electrophoresis

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Lemos, M. Adilia; Teixeira, J. A.; Mota, M.; Gama, F. M. / A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease.

In: Biotechnology Letters, Vol. 22, No. 8, 2000, p. 703-707.

Research output: Contribution to journal › Article

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abstract = "Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.",
author = "Lemos, {M. Adilia} and Teixeira, {J. A.} and M. Mota and Gama, {F. M.}",
year = "2000",
doi = "10.1023/A:1005691821173",
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Lemos, MA, Teixeira, JA, Mota, M & Gama, FM 2000, 'A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease' Biotechnology Letters, vol 22, no. 8, pp. 703-707. DOI: 10.1023/A:1005691821173

A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease. / Lemos, M. Adilia; Teixeira, J. A.; Mota, M.; Gama, F. M.

In: Biotechnology Letters, Vol. 22, No. 8, 2000, p. 703-707.

Research output: Contribution to journal › Article

TY - JOUR

T1 - A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

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AU - Teixeira,J. A.

AU - Mota,M.

AU - Gama,F. M.

PY - 2000

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AB - Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.

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JO - Biotechnology Letters

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JF - Biotechnology Letters

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Lemos MA, Teixeira JA, Mota M, Gama FM. A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease. Biotechnology Letters. 2000;22(8):703-707. Available from, DOI: 10.1023/A:1005691821173

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