A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

M. Adilia Lemos, J. A. Teixeira, M. Mota, F. M. Gama

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.
Original languageEnglish
Pages (from-to)703-707
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number8
DOIs
Publication statusPublished - 2000

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Papain
Cellulases
Capillary electrophoresis
Ultrafiltration
Cellulose
Digestion
Peptide Hydrolases
Trichoderma
Capillary Electrophoresis

Cite this

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title = "A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease",
abstract = "Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85{\%} of binding domains.",
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A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease. / Lemos, M. Adilia; Teixeira, J. A.; Mota, M.; Gama, F. M.

In: Biotechnology Letters, Vol. 22, No. 8, 2000, p. 703-707.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

AU - Lemos, M. Adilia

AU - Teixeira, J. A.

AU - Mota, M.

AU - Gama, F. M.

PY - 2000

Y1 - 2000

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AB - Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.

U2 - 10.1023/A:1005691821173

DO - 10.1023/A:1005691821173

M3 - Article

VL - 22

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EP - 707

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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