A strategy for efficiently collecting aerosol condensate using silica fibers: application to carbonyl emissions from e-cigarettes

W. Edryd Stephens*, Bruna de Falco, Alberto Fiore

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)
    117 Downloads (Pure)

    Abstract

    Analysing harmful constituents in e-cigarette aerosols typically involves adopting a methodology used for analysing tobacco smoke. Cambridge filter pads (CFP) are the basis of numerous protocols for analysing the various classes of compounds representing 93 harmful and potentially harmful constituents identified in tobacco smoke by the FDA. This paper describes a simplified method for trapping the low volatility components of e-cigarette aerosols using a single trapping procedure followed by physical extraction. The trap is a plug of amorphous silica fibres (0.75 g of 4 µm diameter) within a 10mL syringe inserted between the e-cigarette mouthpiece and the pump of the vaping machine. The method is evaluated for emissions from three generations of e-cigarette device (Kangertech CE4, EVOD and Subox Mini-C). On average the silica wool traps about 94% of the vapourised liquid mass in the three devices and higher levels of condensate is retained before reaching saturation compared with CFP. The condensate is then physically extracted from the silica wool plug using a centrifuge. Condensate is then available for use directly in multiple analytical procedures or toxicological experiments. The method is tested by comparison with published analyses of carbonyls, among the most potent toxicants and carcinogens in e-cigarette emissions. Ranges for HPLC-DAD analyses of carbonyl-DNPH derivatives in a laboratory formulation of e-liquid are formaldehyde (0.182±0.023 to 9.896±0.709 µg puff-1), acetaldehyde (0.059±0.005 to 0.791±0.073 µg puff-1) and propionaldehyde (0.008±0.0001 to 0.033±0.023 µg puff-1); other carbonyls are identified and quantified. Carbonyls concentrations are also consistent with published experiments showing marked increases in with variable power settings (10W - 50W). Compared with CFPs, e-cigarette aerosol collection by silica wool requires only one vaping session for multiple analyte groups, traps more condensate per puff, collects more condensate before saturating.
    Original languageEnglish
    Pages (from-to)2053-2062
    Number of pages10
    JournalChemical Research in Toxicology
    Volume32
    Issue number10
    Early online date13 Sep 2019
    DOIs
    Publication statusPublished - 21 Oct 2019

    Keywords

    • Toxicants
    • Formaldehyde
    • Liquid Chromatography
    • Vaping
    • Electronic cigarettes

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