An investigation into the potential use of chelating agents and antibiotics as synchrony inducers in the fission yeast Schizosaccharomyces pombe

Graeme M. Walker, John H. Duffus

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Following the discoveries that the divalent cation ionophore A23187 and the divalent cation chelating agent EDTA can be used to synchronize yeast cell division, a study has been undertaken of the possible use of other chelating agents and antibiotics which interact with divalent cations in controlling cell division in the fission yeast Schizosaccharomyces pombe. All the agents studied (five chelators and two antibiotics) arrested cell division in growing cultures of this yeast, but only sodium pyrophosphate and citrate induced synchrony of cell division. Novobiocin produced a transient inhibition of cell division, treated cells exhibiting "endogenous recovery" in the continued presence of the antibiotic. The results obtained are discussed in relation to the hypothesis that the concentration of intracellular Mg2+ regulates cell division.
    Original languageEnglish
    Pages (from-to)391-400
    Number of pages10
    JournalJournal of General Microbiology
    Volume114
    Issue number2
    DOIs
    Publication statusPublished - 1 Oct 1979

    Keywords

    • Schizosaccharomyces
    • Fission yeast

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