An investigation into the potential use of chelating agents and antibiotics as synchrony inducers in the fission yeast Schizosaccharomyces pombe

Graeme M. Walker, John H. Duffus

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6 Citations (Scopus)

Abstract

Following the discoveries that the divalent cation ionophore A23187 and the divalent cation chelating agent EDTA can be used to synchronize yeast cell division, a study has been undertaken of the possible use of other chelating agents and antibiotics which interact with divalent cations in controlling cell division in the fission yeast Schizosaccharomyces pombe. All the agents studied (five chelators and two antibiotics) arrested cell division in growing cultures of this yeast, but only sodium pyrophosphate and citrate induced synchrony of cell division. Novobiocin produced a transient inhibition of cell division, treated cells exhibiting "endogenous recovery" in the continued presence of the antibiotic. The results obtained are discussed in relation to the hypothesis that the concentration of intracellular Mg2+ regulates cell division.
Original languageEnglish
Pages (from-to)391-400
Number of pages10
JournalJournal of General Microbiology
Volume114
Issue number2
DOIs
Publication statusPublished - 1 Oct 1979

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Schizosaccharomyces pombe
chelating agents
cell division
antibiotics
cations
sodium pyrophosphate
yeasts
novobiocin
sodium citrate
EDTA (chelating agent)
ionophores
cells

Cite this

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abstract = "Following the discoveries that the divalent cation ionophore A23187 and the divalent cation chelating agent EDTA can be used to synchronize yeast cell division, a study has been undertaken of the possible use of other chelating agents and antibiotics which interact with divalent cations in controlling cell division in the fission yeast Schizosaccharomyces pombe. All the agents studied (five chelators and two antibiotics) arrested cell division in growing cultures of this yeast, but only sodium pyrophosphate and citrate induced synchrony of cell division. Novobiocin produced a transient inhibition of cell division, treated cells exhibiting {"}endogenous recovery{"} in the continued presence of the antibiotic. The results obtained are discussed in relation to the hypothesis that the concentration of intracellular Mg2+ regulates cell division.",
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AU - Duffus, John H.

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N2 - Following the discoveries that the divalent cation ionophore A23187 and the divalent cation chelating agent EDTA can be used to synchronize yeast cell division, a study has been undertaken of the possible use of other chelating agents and antibiotics which interact with divalent cations in controlling cell division in the fission yeast Schizosaccharomyces pombe. All the agents studied (five chelators and two antibiotics) arrested cell division in growing cultures of this yeast, but only sodium pyrophosphate and citrate induced synchrony of cell division. Novobiocin produced a transient inhibition of cell division, treated cells exhibiting "endogenous recovery" in the continued presence of the antibiotic. The results obtained are discussed in relation to the hypothesis that the concentration of intracellular Mg2+ regulates cell division.

AB - Following the discoveries that the divalent cation ionophore A23187 and the divalent cation chelating agent EDTA can be used to synchronize yeast cell division, a study has been undertaken of the possible use of other chelating agents and antibiotics which interact with divalent cations in controlling cell division in the fission yeast Schizosaccharomyces pombe. All the agents studied (five chelators and two antibiotics) arrested cell division in growing cultures of this yeast, but only sodium pyrophosphate and citrate induced synchrony of cell division. Novobiocin produced a transient inhibition of cell division, treated cells exhibiting "endogenous recovery" in the continued presence of the antibiotic. The results obtained are discussed in relation to the hypothesis that the concentration of intracellular Mg2+ regulates cell division.

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JF - Critical Reviews in Biotechnology

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