An EU funded collaborative project is constructing an Ultra High Density (UHD) map of potato containing ~10,000 markers. This map, the densest yet made in a crop plant, is being generated from a cross between two heterozygous diploid clones, and will become a vital tool for potato genetics and genomics projects. To demonstrate the utility of the UHD map, we have started to construct a physical map of the region between two RFLP markers on potato linkage group V, which is known to harbour many important genes, several of which confer resistances to major pests and diseases. Moreover, genes affecting traits such as maturity, dormancy and earliness also reside in this region. The mapping strategy uses AFLP markers mapped to the region to seed a 'genetically anchored' physical map. We have developed a multi-dimensional pooling strategy, whereby a BAC library can be screened using AFLP in 200 PCR reactions. Multiple BACs obtained by AFLP screening are then used to initiate 'walks' into the surrounding DNA. BACs are 'skim' sequenced to identify gene content and to facilitate marker development (SSRs and SNPs) for validation of BAC location, fine-scale mapping, map-based gene cloning and marker-trait association studies.
|Number of pages||1|
|Publication status||Published - 2002|
|Event||XVIth EUCARPIA Congress: Plant breeding: sustaining the future - Edinburgh International Conference Centre, Edinburgh, United Kingdom|
Duration: 10 Sep 2001 → 14 Sep 2001
Conference number: 16th
|Conference||XVIth EUCARPIA Congress|
|Period||10/09/01 → 14/09/01|