An Ultra High Density genetic linkage map of potato as platform for targeted physical mapping and map-based cloning

Glenn J. Bryan; Irene Tierney; Dan Milbourne; Robbie Waugh

An Ultra High Density genetic linkage map of potato as platform for targeted physical mapping and map-based cloning

Glenn J. Bryan, Irene Tierney, Dan Milbourne^*, Robbie Waugh (Editor)

^*Corresponding author for this work

Research output: Contribution to conference › Poster

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Abstract

An EU funded collaborative project is constructing an Ultra High Density (UHD) map of potato containing ~10,000 markers. This map, the densest yet made in a crop plant, is being generated from a cross between two heterozygous diploid clones, and will become a vital tool for potato genetics and genomics projects. To demonstrate the utility of the UHD map, we have started to construct a physical map of the region between two RFLP markers on potato linkage group V, which is known to harbour many important genes, several of which confer resistances to major pests and diseases. Moreover, genes affecting traits such as maturity, dormancy and earliness also reside in this region. The mapping strategy uses AFLP markers mapped to the region to seed a 'genetically anchored' physical map. We have developed a multi-dimensional pooling strategy, whereby a BAC library can be screened using AFLP in 200 PCR reactions. Multiple BACs obtained by AFLP screening are then used to initiate 'walks' into the surrounding DNA. BACs are 'skim' sequenced to identify gene content and to facilitate marker development (SSRs and SNPs) for validation of BAC location, fine-scale mapping, map-based gene cloning and marker-trait association studies.

Original language	English
Number of pages	1
Publication status	Published - 2002
Externally published	Yes
Event	XVIth EUCARPIA Congress: Plant breeding: sustaining the future - Edinburgh International Conference Centre, Edinburgh, United Kingdom Duration: 10 Sep 2001 → 14 Sep 2001 Conference number: 16th

Conference

Conference	XVIth EUCARPIA Congress
Country	United Kingdom
City	Edinburgh
Period	10/09/01 → 14/09/01

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Bryan, G. J., Tierney, I., Milbourne, D., & Waugh, R. (Ed.) (2002). An Ultra High Density genetic linkage map of potato as platform for targeted physical mapping and map-based cloning. Poster session presented at XVIth EUCARPIA Congress, Edinburgh, United Kingdom. https://www.hutton.ac.uk/webfm_send/144

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title = "An Ultra High Density genetic linkage map of potato as platform for targeted physical mapping and map-based cloning",

abstract = "An EU funded collaborative project is constructing an Ultra High Density (UHD) map of potato containing ~10,000 markers. This map, the densest yet made in a crop plant, is being generated from a cross between two heterozygous diploid clones, and will become a vital tool for potato genetics and genomics projects. To demonstrate the utility of the UHD map, we have started to construct a physical map of the region between two RFLP markers on potato linkage group V, which is known to harbour many important genes, several of which confer resistances to major pests and diseases. Moreover, genes affecting traits such as maturity, dormancy and earliness also reside in this region. The mapping strategy uses AFLP markers mapped to the region to seed a 'genetically anchored' physical map. We have developed a multi-dimensional pooling strategy, whereby a BAC library can be screened using AFLP in 200 PCR reactions. Multiple BACs obtained by AFLP screening are then used to initiate 'walks' into the surrounding DNA. BACs are 'skim' sequenced to identify gene content and to facilitate marker development (SSRs and SNPs) for validation of BAC location, fine-scale mapping, map-based gene cloning and marker-trait association studies.",

author = "Bryan, {Glenn J.} and Irene Tierney and Dan Milbourne and Robbie Waugh",

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language = "English",

note = "XVIth EUCARPIA Congress : Plant breeding: sustaining the future ; Conference date: 10-09-2001 Through 14-09-2001",

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AU - Bryan, Glenn J.

AU - Tierney, Irene

AU - Milbourne, Dan

A2 - Waugh, Robbie

N1 - Conference code: 16th

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N2 - An EU funded collaborative project is constructing an Ultra High Density (UHD) map of potato containing ~10,000 markers. This map, the densest yet made in a crop plant, is being generated from a cross between two heterozygous diploid clones, and will become a vital tool for potato genetics and genomics projects. To demonstrate the utility of the UHD map, we have started to construct a physical map of the region between two RFLP markers on potato linkage group V, which is known to harbour many important genes, several of which confer resistances to major pests and diseases. Moreover, genes affecting traits such as maturity, dormancy and earliness also reside in this region. The mapping strategy uses AFLP markers mapped to the region to seed a 'genetically anchored' physical map. We have developed a multi-dimensional pooling strategy, whereby a BAC library can be screened using AFLP in 200 PCR reactions. Multiple BACs obtained by AFLP screening are then used to initiate 'walks' into the surrounding DNA. BACs are 'skim' sequenced to identify gene content and to facilitate marker development (SSRs and SNPs) for validation of BAC location, fine-scale mapping, map-based gene cloning and marker-trait association studies.

AB - An EU funded collaborative project is constructing an Ultra High Density (UHD) map of potato containing ~10,000 markers. This map, the densest yet made in a crop plant, is being generated from a cross between two heterozygous diploid clones, and will become a vital tool for potato genetics and genomics projects. To demonstrate the utility of the UHD map, we have started to construct a physical map of the region between two RFLP markers on potato linkage group V, which is known to harbour many important genes, several of which confer resistances to major pests and diseases. Moreover, genes affecting traits such as maturity, dormancy and earliness also reside in this region. The mapping strategy uses AFLP markers mapped to the region to seed a 'genetically anchored' physical map. We have developed a multi-dimensional pooling strategy, whereby a BAC library can be screened using AFLP in 200 PCR reactions. Multiple BACs obtained by AFLP screening are then used to initiate 'walks' into the surrounding DNA. BACs are 'skim' sequenced to identify gene content and to facilitate marker development (SSRs and SNPs) for validation of BAC location, fine-scale mapping, map-based gene cloning and marker-trait association studies.

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