Anaerobic digestion of distillery spent wash: influence of enzymatic pre-treatment of intact yeast cells

Research output: Contribution to journalArticle

  • 10 Citations

Abstract

The potential benefits of enzymatic digestion of intact yeast cells on anaerobic digestion of Scotch whisky distillery spent wash and pot ale were investigated. Various yeast cell wall hydrolytic enzymes were studied based on their effect on dissolution of cell wall glucan and mannoprotein. The synergistic activity of beta-glucanase and protease showed greater than 90% yeast cell digestion at 37 °C in 24 h. The widely-used industrial enzyme papain showed 95% yeast cell digestion in spent wash at 1% enzyme concentration within 22 h at 50 °C. Anaerobic digestion of pot ale residues containing intact yeast cells pre-treated with lytic enzymes showed COD reductions of 87%, compared with only 13% without enzymes. Similar results were observed with distillery spent wash centrate. The hydrolysis of intact yeast cells in distillery liquid residues was found to be a rate-limiting step in anaerobic treatment of such residues.
Original languageEnglish
Pages (from-to)1681-1685
Number of pages5
JournalBioresource Technology
Volume101
Issue number6
DOIs
StatePublished - Mar 2010

Fingerprint

Cells
Castration
yeast
Yeast
HLA Antigens
digestion
enzyme
Enzymes
Distilleries
Anaerobic digestion
hydrolysis
dissolution
liquid
Papain
Hydrolysis
Dissolution
Liquids
Flicker Fusion
Birth Certificates

Cite this

Mallick, P.; Akunna, Joseph C.; Walker, Graeme M. / Anaerobic digestion of distillery spent wash : influence of enzymatic pre-treatment of intact yeast cells.

In: Bioresource Technology, Vol. 101, No. 6, 03.2010, p. 1681-1685.

Research output: Contribution to journalArticle

@article{a25be3f47ffc4a13bfbd4810a436326d,
title = "Anaerobic digestion of distillery spent wash: influence of enzymatic pre-treatment of intact yeast cells",
abstract = "The potential benefits of enzymatic digestion of intact yeast cells on anaerobic digestion of Scotch whisky distillery spent wash and pot ale were investigated. Various yeast cell wall hydrolytic enzymes were studied based on their effect on dissolution of cell wall glucan and mannoprotein. The synergistic activity of beta-glucanase and protease showed greater than 90% yeast cell digestion at 37 °C in 24 h. The widely-used industrial enzyme papain showed 95% yeast cell digestion in spent wash at 1% enzyme concentration within 22 h at 50 °C. Anaerobic digestion of pot ale residues containing intact yeast cells pre-treated with lytic enzymes showed COD reductions of 87%, compared with only 13% without enzymes. Similar results were observed with distillery spent wash centrate. The hydrolysis of intact yeast cells in distillery liquid residues was found to be a rate-limiting step in anaerobic treatment of such residues.",
author = "P. Mallick and Akunna, {Joseph C.} and Walker, {Graeme M.}",
year = "2010",
month = "3",
doi = "10.1016/j.biortech.2009.09.089",
volume = "101",
pages = "1681--1685",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",
number = "6",

}

Anaerobic digestion of distillery spent wash : influence of enzymatic pre-treatment of intact yeast cells. / Mallick, P.; Akunna, Joseph C.; Walker, Graeme M.

In: Bioresource Technology, Vol. 101, No. 6, 03.2010, p. 1681-1685.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anaerobic digestion of distillery spent wash

T2 - Bioresource Technology

AU - Mallick,P.

AU - Akunna,Joseph C.

AU - Walker,Graeme M.

PY - 2010/3

Y1 - 2010/3

N2 - The potential benefits of enzymatic digestion of intact yeast cells on anaerobic digestion of Scotch whisky distillery spent wash and pot ale were investigated. Various yeast cell wall hydrolytic enzymes were studied based on their effect on dissolution of cell wall glucan and mannoprotein. The synergistic activity of beta-glucanase and protease showed greater than 90% yeast cell digestion at 37 °C in 24 h. The widely-used industrial enzyme papain showed 95% yeast cell digestion in spent wash at 1% enzyme concentration within 22 h at 50 °C. Anaerobic digestion of pot ale residues containing intact yeast cells pre-treated with lytic enzymes showed COD reductions of 87%, compared with only 13% without enzymes. Similar results were observed with distillery spent wash centrate. The hydrolysis of intact yeast cells in distillery liquid residues was found to be a rate-limiting step in anaerobic treatment of such residues.

AB - The potential benefits of enzymatic digestion of intact yeast cells on anaerobic digestion of Scotch whisky distillery spent wash and pot ale were investigated. Various yeast cell wall hydrolytic enzymes were studied based on their effect on dissolution of cell wall glucan and mannoprotein. The synergistic activity of beta-glucanase and protease showed greater than 90% yeast cell digestion at 37 °C in 24 h. The widely-used industrial enzyme papain showed 95% yeast cell digestion in spent wash at 1% enzyme concentration within 22 h at 50 °C. Anaerobic digestion of pot ale residues containing intact yeast cells pre-treated with lytic enzymes showed COD reductions of 87%, compared with only 13% without enzymes. Similar results were observed with distillery spent wash centrate. The hydrolysis of intact yeast cells in distillery liquid residues was found to be a rate-limiting step in anaerobic treatment of such residues.

U2 - 10.1016/j.biortech.2009.09.089

DO - 10.1016/j.biortech.2009.09.089

M3 - Article

VL - 101

SP - 1681

EP - 1685

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

IS - 6

ER -