Anaerobic digestion of distillery spent wash: influence of enzymatic pre-treatment of intact yeast cells

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    The potential benefits of enzymatic digestion of intact yeast cells on anaerobic digestion of Scotch whisky distillery spent wash and pot ale were investigated. Various yeast cell wall hydrolytic enzymes were studied based on their effect on dissolution of cell wall glucan and mannoprotein. The synergistic activity of beta-glucanase and protease showed greater than 90% yeast cell digestion at 37 °C in 24 h. The widely-used industrial enzyme papain showed 95% yeast cell digestion in spent wash at 1% enzyme concentration within 22 h at 50 °C. Anaerobic digestion of pot ale residues containing intact yeast cells pre-treated with lytic enzymes showed COD reductions of 87%, compared with only 13% without enzymes. Similar results were observed with distillery spent wash centrate. The hydrolysis of intact yeast cells in distillery liquid residues was found to be a rate-limiting step in anaerobic treatment of such residues.
    Original languageEnglish
    Pages (from-to)1681-1685
    Number of pages5
    JournalBioresource Technology
    Volume101
    Issue number6
    DOIs
    Publication statusPublished - Mar 2010

    Keywords

    • COD reduction
    • Anaerobic digestion
    • Distillery spent wash centrate
    • Distillery pot ale
    • Enzymatic hydrolysis
    • Yeast cell lysis

    Fingerprint

    Dive into the research topics of 'Anaerobic digestion of distillery spent wash: influence of enzymatic pre-treatment of intact yeast cells'. Together they form a unique fingerprint.

    Cite this