Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength

Jorge A. Saraiva, Jorge C. Oliveira, M. Adilia Lemos, Marc E. Hendrickx

Research output: Contribution to journalArticle

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Abstract

The thermal inactivation of horseradish peroxidase was studied in sodium phosphate buffer solutions and in pure water at pH 7 in the temperature range of 70–95°C. The sodium phosphate ions concentration affected both the thermostability and the kinetic patterns and had a stabilizing effect. The gradual change observed at low concentrations made a series-type mechanism theoretically more coherent with the experimental observations than the conventionally applied two-fraction model. In water the kinetics is apparently First order at high temperatures, while the results obtained at 25°C support the occurrence of a series-type inactivation mechanism. The pH and enzyme concentration also affect the inactivation proFile, supporting the conclusion that the thermal inactivation is not a monomolecular process with respect to protein concentration.
Original languageEnglish
Pages (from-to)223–231
Number of pages9
JournalInternational Journal of Food Science and Technology
Volume31
Issue number3
DOIs
StatePublished - May 1996

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Horseradish Peroxidase
Osmolar Concentration
Buffers
Hot Temperature
Sodium
Phosphates
Temperature
Water
sodium phosphate
heat inactivation
kinetics
Kinetics
inactivation
peroxidase
buffers
temperature
water
Ions
Enzymes
Proteins

Cite this

Saraiva, Jorge A.; Oliveira, Jorge C.; Lemos, M. Adilia; Hendrickx, Marc E. / Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength.

In: International Journal of Food Science and Technology, Vol. 31, No. 3, 05.1996, p. 223–231.

Research output: Contribution to journalArticle

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Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength. / Saraiva, Jorge A.; Oliveira, Jorge C.; Lemos, M. Adilia; Hendrickx, Marc E.

In: International Journal of Food Science and Technology, Vol. 31, No. 3, 05.1996, p. 223–231.

Research output: Contribution to journalArticle

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AU - Oliveira,Jorge C.

AU - Lemos,M. Adilia

AU - Hendrickx,Marc E.

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