Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1

Suzanne M. Abdel Malek, I. S. I. Al-Adham, C. L. Winder, T. E. J. Buultjens, Kevan M. A. Gartland, Phillip J. Collier

Research output: Contribution to journalArticle

  • 14 Citations

Abstract

Aims: The aim of this study was to determine whether passaging Pseudomonas aeruginosa PAO1 with sub-MICs of the pyrithione biocides results in both the induction of decreased susceptibility towards these antimicrobials and associated outer membrane profile changes. Methods and Results: Previous work by this group has shown that it is possible to induce susceptibility changes towards the isothiazolone biocides in Ps. aeruginosa PAO1 by successive passages in the presence of increasing sub-MICs of biocide. This procedure was accompanied by the loss of a 35 kDa outer membrane protein, T-OMP. In this experiment, this process was repeated with the biocides sodium pyrithione (NaPT), zinc pyrithione (ZnPT) and cetrimide. The pattern of susceptibility was similar to that observed with the isothiazolone biocides. Upon removal of biocide, the observed MIC did not return to the original pre-exposure value. The onset and development of resistance was accompanied by the loss of T-OMP from outer membrane profiles, which suggests that this is a non-specific membrane channel whose production within the cell is sensitive to biocide presence. The T-OMP reappeared when the cells were passaged in the absence of pyrithione. Cross-resistance studies indicated that induced resistance to one biocide yields partial resistance towards other members of the group and the positive control. Conclusions: These results indicate that the pyrithione biocides have similar susceptibility profiles in Ps. aeruginosa to those exhibited by the isothiazolones, but that the acquired changes in susceptibility to the pyrithiones is largely irreversible. Significance and Impact of the Study: This study indicates that acquired susceptibility changes towards sub-MICs of selected biocides are multifactorial in nature.
Original languageEnglish
Pages (from-to)729-736
Number of pages8
JournalJournal of Applied Microbiology
Volume92
Issue number4
DOIs
StatePublished - Apr 2002

Fingerprint

Disinfectants
Pseudomonas aeruginosa
Membranes
Ion Channels
Zinc
Membrane Proteins
Sodium

Cite this

Abdel Malek, S. M., Al-Adham, I. S. I., Winder, C. L., Buultjens, T. E. J., Gartland, K. M. A., & Collier, P. J. (2002). Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1. Journal of Applied Microbiology, 92(4), 729-736. DOI: 10.1046/j.1365-2672.2002.01575.x

Abdel Malek, Suzanne M.; Al-Adham, I. S. I.; Winder, C. L.; Buultjens, T. E. J.; Gartland, Kevan M. A.; Collier, Phillip J. / Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1.

In: Journal of Applied Microbiology, Vol. 92, No. 4, 04.2002, p. 729-736.

Research output: Contribution to journalArticle

@article{abf48ec785784703a7594ff406a06d6f,
title = "Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1",
abstract = "Aims: The aim of this study was to determine whether passaging Pseudomonas aeruginosa PAO1 with sub-MICs of the pyrithione biocides results in both the induction of decreased susceptibility towards these antimicrobials and associated outer membrane profile changes. Methods and Results: Previous work by this group has shown that it is possible to induce susceptibility changes towards the isothiazolone biocides in Ps. aeruginosa PAO1 by successive passages in the presence of increasing sub-MICs of biocide. This procedure was accompanied by the loss of a 35 kDa outer membrane protein, T-OMP. In this experiment, this process was repeated with the biocides sodium pyrithione (NaPT), zinc pyrithione (ZnPT) and cetrimide. The pattern of susceptibility was similar to that observed with the isothiazolone biocides. Upon removal of biocide, the observed MIC did not return to the original pre-exposure value. The onset and development of resistance was accompanied by the loss of T-OMP from outer membrane profiles, which suggests that this is a non-specific membrane channel whose production within the cell is sensitive to biocide presence. The T-OMP reappeared when the cells were passaged in the absence of pyrithione. Cross-resistance studies indicated that induced resistance to one biocide yields partial resistance towards other members of the group and the positive control. Conclusions: These results indicate that the pyrithione biocides have similar susceptibility profiles in Ps. aeruginosa to those exhibited by the isothiazolones, but that the acquired changes in susceptibility to the pyrithiones is largely irreversible. Significance and Impact of the Study: This study indicates that acquired susceptibility changes towards sub-MICs of selected biocides are multifactorial in nature.",
author = "{Abdel Malek}, {Suzanne M.} and Al-Adham, {I. S. I.} and Winder, {C. L.} and Buultjens, {T. E. J.} and Gartland, {Kevan M. A.} and Collier, {Phillip J.}",
year = "2002",
month = "4",
doi = "10.1046/j.1365-2672.2002.01575.x",
volume = "92",
pages = "729--736",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "4",

}

Abdel Malek, SM, Al-Adham, ISI, Winder, CL, Buultjens, TEJ, Gartland, KMA & Collier, PJ 2002, 'Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1' Journal of Applied Microbiology, vol 92, no. 4, pp. 729-736. DOI: 10.1046/j.1365-2672.2002.01575.x

Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1. / Abdel Malek, Suzanne M.; Al-Adham, I. S. I.; Winder, C. L.; Buultjens, T. E. J.; Gartland, Kevan M. A.; Collier, Phillip J.

In: Journal of Applied Microbiology, Vol. 92, No. 4, 04.2002, p. 729-736.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1

AU - Abdel Malek,Suzanne M.

AU - Al-Adham,I. S. I.

AU - Winder,C. L.

AU - Buultjens,T. E. J.

AU - Gartland,Kevan M. A.

AU - Collier,Phillip J.

PY - 2002/4

Y1 - 2002/4

N2 - Aims: The aim of this study was to determine whether passaging Pseudomonas aeruginosa PAO1 with sub-MICs of the pyrithione biocides results in both the induction of decreased susceptibility towards these antimicrobials and associated outer membrane profile changes. Methods and Results: Previous work by this group has shown that it is possible to induce susceptibility changes towards the isothiazolone biocides in Ps. aeruginosa PAO1 by successive passages in the presence of increasing sub-MICs of biocide. This procedure was accompanied by the loss of a 35 kDa outer membrane protein, T-OMP. In this experiment, this process was repeated with the biocides sodium pyrithione (NaPT), zinc pyrithione (ZnPT) and cetrimide. The pattern of susceptibility was similar to that observed with the isothiazolone biocides. Upon removal of biocide, the observed MIC did not return to the original pre-exposure value. The onset and development of resistance was accompanied by the loss of T-OMP from outer membrane profiles, which suggests that this is a non-specific membrane channel whose production within the cell is sensitive to biocide presence. The T-OMP reappeared when the cells were passaged in the absence of pyrithione. Cross-resistance studies indicated that induced resistance to one biocide yields partial resistance towards other members of the group and the positive control. Conclusions: These results indicate that the pyrithione biocides have similar susceptibility profiles in Ps. aeruginosa to those exhibited by the isothiazolones, but that the acquired changes in susceptibility to the pyrithiones is largely irreversible. Significance and Impact of the Study: This study indicates that acquired susceptibility changes towards sub-MICs of selected biocides are multifactorial in nature.

AB - Aims: The aim of this study was to determine whether passaging Pseudomonas aeruginosa PAO1 with sub-MICs of the pyrithione biocides results in both the induction of decreased susceptibility towards these antimicrobials and associated outer membrane profile changes. Methods and Results: Previous work by this group has shown that it is possible to induce susceptibility changes towards the isothiazolone biocides in Ps. aeruginosa PAO1 by successive passages in the presence of increasing sub-MICs of biocide. This procedure was accompanied by the loss of a 35 kDa outer membrane protein, T-OMP. In this experiment, this process was repeated with the biocides sodium pyrithione (NaPT), zinc pyrithione (ZnPT) and cetrimide. The pattern of susceptibility was similar to that observed with the isothiazolone biocides. Upon removal of biocide, the observed MIC did not return to the original pre-exposure value. The onset and development of resistance was accompanied by the loss of T-OMP from outer membrane profiles, which suggests that this is a non-specific membrane channel whose production within the cell is sensitive to biocide presence. The T-OMP reappeared when the cells were passaged in the absence of pyrithione. Cross-resistance studies indicated that induced resistance to one biocide yields partial resistance towards other members of the group and the positive control. Conclusions: These results indicate that the pyrithione biocides have similar susceptibility profiles in Ps. aeruginosa to those exhibited by the isothiazolones, but that the acquired changes in susceptibility to the pyrithiones is largely irreversible. Significance and Impact of the Study: This study indicates that acquired susceptibility changes towards sub-MICs of selected biocides are multifactorial in nature.

U2 - 10.1046/j.1365-2672.2002.01575.x

DO - 10.1046/j.1365-2672.2002.01575.x

M3 - Article

VL - 92

SP - 729

EP - 736

JO - Journal of Applied Microbiology

T2 - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 4

ER -

Abdel Malek SM, Al-Adham ISI, Winder CL, Buultjens TEJ, Gartland KMA, Collier PJ. Antimicrobial susceptibility changes and T-OMP shifts in pyrithione-passaged planktonic cultures of Pseudomonas aeruginosa PAO1. Journal of Applied Microbiology. 2002 Apr;92(4):729-736. Available from, DOI: 10.1046/j.1365-2672.2002.01575.x