Atomic force microscopic study of the effects of ethanol on yeast cell surface morphology

Elisabetta Canetta, Ashok K. Adya, Graeme M. Walker

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    43 Citations (Scopus)

    Abstract

    The detrimental effects of ethanol toxicity on the cell surface morphology of Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354) were investigated using an atomic force microscope (AFM). In combination with culture viability and mean cell volume measurements AFM studies allowed us to relate the cell surface morphological changes, observed on nanometer lateral resolution, with the cellular stress physiology. Exposing yeasts to increasing stressful concentrations of ethanol led to decreased cell viabilities and mean cell volumes. Together with the roughness and bearing volume analyses of the AFM images, the results provided novel insight into the relative ethanol tolerance of S. cerevisiae and Sc. pombe.
    Original languageEnglish
    Pages (from-to)308-315
    Number of pages8
    JournalFEMS Microbiology Letters
    Volume255
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 2006

    Keywords

    • AFM
    • Atomic force microscopy
    • Cell morphology
    • Cell physiology
    • Ethanol stress
    • Yeasts

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