Abstract
| Language | English |
|---|---|
| Pages | 108-113 |
| Number of pages | 6 |
| Journal | Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy |
| Volume | 211 |
| Early online date | 30 Nov 2018 |
| DOIs | |
| Publication status | E-pub ahead of print - 30 Nov 2018 |
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Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach. / Hungerford, Graham; Lemos, M. Adilia; Chu, Boon-Seang.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 211, 15.03.2019, p. 108-113.Research output: Contribution to journal › Article
TY - JOUR
T1 - Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach
AU - Hungerford, Graham
AU - Lemos, M. Adilia
AU - Chu, Boon-Seang
PY - 2018/11/30
Y1 - 2018/11/30
N2 - The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. Since the extract contains a mixture of flavonoids, including anthocyanins, in order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.
AB - The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. Since the extract contains a mixture of flavonoids, including anthocyanins, in order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.
U2 - 10.1016/j.saa.2018.11.062
DO - 10.1016/j.saa.2018.11.062
M3 - Article
VL - 211
SP - 108
EP - 113
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
T2 - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
SN - 1386-1425
ER -