Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach

Graham Hungerford, M. Adilia Lemos, Boon-Seang Chu

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    Abstract

    The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. Since the extract contains a mixture of flavonoids, including anthocyanins, in order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.
    Original languageEnglish
    Pages (from-to)108-113
    Number of pages6
    JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
    Volume211
    Early online date30 Nov 2018
    DOIs
    Publication statusPublished - 15 Mar 2019

    Fingerprint

    Anthocyanins
    Flavonoids
    Amylases
    Photons
    Fluorescence
    Wavelength
    fluorescence
    photons
    wavelengths
    enzyme activity
    starches
    Enzyme activity
    Starch
    enzymes
    Enzymes
    life (durability)
    Kinetics
    kinetics
    decay
    interactions

    Cite this

    Hungerford, Graham ; Lemos, M. Adilia ; Chu, Boon-Seang. / Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach. In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2019 ; Vol. 211. pp. 108-113.
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    title = "Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach",
    abstract = "The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. Since the extract contains a mixture of flavonoids, including anthocyanins, in order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.",
    author = "Graham Hungerford and Lemos, {M. Adilia} and Boon-Seang Chu",
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    Hungerford, G, Lemos, MA & Chu, B-S 2019, 'Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach', Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 211, pp. 108-113. https://doi.org/10.1016/j.saa.2018.11.062

    Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach. / Hungerford, Graham; Lemos, M. Adilia; Chu, Boon-Seang.

    In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 211, 15.03.2019, p. 108-113.

    Research output: Contribution to journal › Article

    TY - JOUR

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    AU - Hungerford, Graham

    AU - Lemos, M. Adilia

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    N2 - The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. Since the extract contains a mixture of flavonoids, including anthocyanins, in order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.

    AB - The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. Since the extract contains a mixture of flavonoids, including anthocyanins, in order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra.

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    Hungerford G, Lemos MA, Chu B-S. Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2019 Mar 15;211:108-113. https://doi.org/10.1016/j.saa.2018.11.062

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