Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1

Alberto Fiore, Luisa Mannina, Anatoli P. Sobolev, Anna Maria Salzano, Andrea Scaloni, Ingeborg Grgurina, Maria Rosaria Fullone, Monica Gallo, Camille Swasey, Vincenzo Fogliano, Jon Y. Takemoto*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The production of secondary metabolite lipopeptides by ice-nucleating Pseudomonas syringae strain 31R1 was investigated. Pseudomonas syringae strain 31R1 is a rifampicin-resistant derivative of P. syringae no. 31 used for the commercial production of snow. It is shown that P. syringae strain 31R1 produces antifungal lipodepsipeptides, syringomycins E and G, and, in addition, a novel and unique lipopeptide, peptin31. Spectroscopic and spectrometric analyses revealed that peptin31 is a linear undecalipopeptide with sequence identities to N- and C-terminal portions but lacking 11 amino acids of known lipodepsipeptide syringopeptin SPPhv. Peptin31 displayed antifungal activities against Rhodotorula pilimanae, Rhizoctonia solani, and Trichoderma harzianum and also hemolytic and antibacterial activities. Extracts of P. syringae strain 31R1 grown in medium with chloride were fungicidal, but not when grown without chloride. The latter extracts lacked peptin 31 and contained des-chloro forms of syringomycins E and G with low antifungal activities. Thus, the three lipopeptides account for the fungicidal properties of P. syringae 31R1 extracts. The occurrence of these bioactive metabolites should be considered when P. syringae no. 31 and its derivatives are used in products for making artificial snow.

Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalFEMS Microbiology Letters
Volume286
Issue number2
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Cite this

Fiore, A., Mannina, L., Sobolev, A. P., Salzano, A. M., Scaloni, A., Grgurina, I., ... Takemoto, J. Y. (2008). Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1. FEMS Microbiology Letters, 286(2), 158-165. https://doi.org/10.1111/j.1574-6968.2008.01247.x
Fiore, Alberto ; Mannina, Luisa ; Sobolev, Anatoli P. ; Salzano, Anna Maria ; Scaloni, Andrea ; Grgurina, Ingeborg ; Fullone, Maria Rosaria ; Gallo, Monica ; Swasey, Camille ; Fogliano, Vincenzo ; Takemoto, Jon Y. / Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1. In: FEMS Microbiology Letters. 2008 ; Vol. 286, No. 2. pp. 158-165.
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abstract = "The production of secondary metabolite lipopeptides by ice-nucleating Pseudomonas syringae strain 31R1 was investigated. Pseudomonas syringae strain 31R1 is a rifampicin-resistant derivative of P. syringae no. 31 used for the commercial production of snow. It is shown that P. syringae strain 31R1 produces antifungal lipodepsipeptides, syringomycins E and G, and, in addition, a novel and unique lipopeptide, peptin31. Spectroscopic and spectrometric analyses revealed that peptin31 is a linear undecalipopeptide with sequence identities to N- and C-terminal portions but lacking 11 amino acids of known lipodepsipeptide syringopeptin SPPhv. Peptin31 displayed antifungal activities against Rhodotorula pilimanae, Rhizoctonia solani, and Trichoderma harzianum and also hemolytic and antibacterial activities. Extracts of P. syringae strain 31R1 grown in medium with chloride were fungicidal, but not when grown without chloride. The latter extracts lacked peptin 31 and contained des-chloro forms of syringomycins E and G with low antifungal activities. Thus, the three lipopeptides account for the fungicidal properties of P. syringae 31R1 extracts. The occurrence of these bioactive metabolites should be considered when P. syringae no. 31 and its derivatives are used in products for making artificial snow.",
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Fiore, A, Mannina, L, Sobolev, AP, Salzano, AM, Scaloni, A, Grgurina, I, Fullone, MR, Gallo, M, Swasey, C, Fogliano, V & Takemoto, JY 2008, 'Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1', FEMS Microbiology Letters, vol. 286, no. 2, pp. 158-165. https://doi.org/10.1111/j.1574-6968.2008.01247.x

Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1. / Fiore, Alberto; Mannina, Luisa; Sobolev, Anatoli P.; Salzano, Anna Maria; Scaloni, Andrea; Grgurina, Ingeborg; Fullone, Maria Rosaria; Gallo, Monica; Swasey, Camille; Fogliano, Vincenzo; Takemoto, Jon Y.

In: FEMS Microbiology Letters, Vol. 286, No. 2, 09.2008, p. 158-165.

Research output: Contribution to journalArticle

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T1 - Bioactive lipopeptides of ice-nucleating snow bacterium Pseudomonas syringae strain 31R1

AU - Fiore, Alberto

AU - Mannina, Luisa

AU - Sobolev, Anatoli P.

AU - Salzano, Anna Maria

AU - Scaloni, Andrea

AU - Grgurina, Ingeborg

AU - Fullone, Maria Rosaria

AU - Gallo, Monica

AU - Swasey, Camille

AU - Fogliano, Vincenzo

AU - Takemoto, Jon Y.

PY - 2008/9

Y1 - 2008/9

N2 - The production of secondary metabolite lipopeptides by ice-nucleating Pseudomonas syringae strain 31R1 was investigated. Pseudomonas syringae strain 31R1 is a rifampicin-resistant derivative of P. syringae no. 31 used for the commercial production of snow. It is shown that P. syringae strain 31R1 produces antifungal lipodepsipeptides, syringomycins E and G, and, in addition, a novel and unique lipopeptide, peptin31. Spectroscopic and spectrometric analyses revealed that peptin31 is a linear undecalipopeptide with sequence identities to N- and C-terminal portions but lacking 11 amino acids of known lipodepsipeptide syringopeptin SPPhv. Peptin31 displayed antifungal activities against Rhodotorula pilimanae, Rhizoctonia solani, and Trichoderma harzianum and also hemolytic and antibacterial activities. Extracts of P. syringae strain 31R1 grown in medium with chloride were fungicidal, but not when grown without chloride. The latter extracts lacked peptin 31 and contained des-chloro forms of syringomycins E and G with low antifungal activities. Thus, the three lipopeptides account for the fungicidal properties of P. syringae 31R1 extracts. The occurrence of these bioactive metabolites should be considered when P. syringae no. 31 and its derivatives are used in products for making artificial snow.

AB - The production of secondary metabolite lipopeptides by ice-nucleating Pseudomonas syringae strain 31R1 was investigated. Pseudomonas syringae strain 31R1 is a rifampicin-resistant derivative of P. syringae no. 31 used for the commercial production of snow. It is shown that P. syringae strain 31R1 produces antifungal lipodepsipeptides, syringomycins E and G, and, in addition, a novel and unique lipopeptide, peptin31. Spectroscopic and spectrometric analyses revealed that peptin31 is a linear undecalipopeptide with sequence identities to N- and C-terminal portions but lacking 11 amino acids of known lipodepsipeptide syringopeptin SPPhv. Peptin31 displayed antifungal activities against Rhodotorula pilimanae, Rhizoctonia solani, and Trichoderma harzianum and also hemolytic and antibacterial activities. Extracts of P. syringae strain 31R1 grown in medium with chloride were fungicidal, but not when grown without chloride. The latter extracts lacked peptin 31 and contained des-chloro forms of syringomycins E and G with low antifungal activities. Thus, the three lipopeptides account for the fungicidal properties of P. syringae 31R1 extracts. The occurrence of these bioactive metabolites should be considered when P. syringae no. 31 and its derivatives are used in products for making artificial snow.

U2 - 10.1111/j.1574-6968.2008.01247.x

DO - 10.1111/j.1574-6968.2008.01247.x

M3 - Article

VL - 286

SP - 158

EP - 165

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

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