Biofilm formation and cellulose expression by Bordetella avium 197N, the causative agent of bordetellosis in birds and an opportunistic respiratory pathogen in humans

Kimberley McLaughlin, Ayorinde O. Folorunso, Yusuf Y. Deeni, Dona Foster, Oksana Gorbatiuk, Simona M. Hapca, Corinna Immoor, Anna Koza, Ibrahim Mohammed, Olena Moshynets, Sergii Rogalsky, Kamil Zawadzki, Andrew J. Spiers

Research output: Contribution to journalArticle

3 Citations (Scopus)
8 Downloads (Pure)

Abstract

Although bacterial cellulose synthase (bcs) operons are widespread within the Proteobacteria phylum, subunits required for the partial-acetylation of the polymer appear to be restricted to a few γ-group soil, plant-associated and phytopathogenic pseudomonads, including Pseudomonas fluorescens SBW25 and several Pseudomonas syringae pathovars. However, a bcs operon with acetylation subunits has also been annotated in the unrelated β-group respiratory pathogen, Bordetella avium 197N. Our comparison of subunit protein sequences and GC content analyses confirms the close similarity between the B. avium 197N and pseudomonad operons and suggests that, in both cases, the cellulose synthase and acetylation subunits were acquired as a single unit. Using static liquid microcosms, we can confirm that B. avium 197N expresses low levels of cellulose in air–liquid interface biofilms and that biofilm strength and attachment levels could be increased by elevating c-di-GMP levels like the pseudomonads, but cellulose was not required for biofilm formation itself. The finding that B. avium 197N is capable of producing cellulose from a highly-conserved, but relatively uncommon bcs operon raises the question of what functional role this modified polymer plays during the infection of the upper respiratory tract or survival between hosts, and what environmental signals control its production.
Original languageEnglish
Pages (from-to)419–430
Number of pages12
JournalResearch in Microbiology
Volume168
Issue number5
Early online date26 Jan 2017
DOIs
Publication statusPublished - Jun 2017

Fingerprint

Bordetella avium
Operon
Biofilms
Cellulose
Birds
Acetylation
Polymers
Pseudomonas syringae
Proteobacteria
Pseudomonas fluorescens
Protein Subunits
Base Composition
Respiratory Tract Infections
Soil
Survival
cellulose synthase

Cite this

McLaughlin, Kimberley ; Folorunso, Ayorinde O. ; Deeni, Yusuf Y. ; Foster, Dona ; Gorbatiuk, Oksana ; Hapca, Simona M. ; Immoor, Corinna ; Koza, Anna ; Mohammed, Ibrahim ; Moshynets, Olena ; Rogalsky, Sergii ; Zawadzki, Kamil ; Spiers, Andrew J. / Biofilm formation and cellulose expression by Bordetella avium 197N, the causative agent of bordetellosis in birds and an opportunistic respiratory pathogen in humans. In: Research in Microbiology. 2017 ; Vol. 168, No. 5. pp. 419–430.
@article{005762bb907f4f438844be1915e6b244,
title = "Biofilm formation and cellulose expression by Bordetella avium 197N, the causative agent of bordetellosis in birds and an opportunistic respiratory pathogen in humans",
abstract = "Although bacterial cellulose synthase (bcs) operons are widespread within the Proteobacteria phylum, subunits required for the partial-acetylation of the polymer appear to be restricted to a few γ-group soil, plant-associated and phytopathogenic pseudomonads, including Pseudomonas fluorescens SBW25 and several Pseudomonas syringae pathovars. However, a bcs operon with acetylation subunits has also been annotated in the unrelated β-group respiratory pathogen, Bordetella avium 197N. Our comparison of subunit protein sequences and GC content analyses confirms the close similarity between the B. avium 197N and pseudomonad operons and suggests that, in both cases, the cellulose synthase and acetylation subunits were acquired as a single unit. Using static liquid microcosms, we can confirm that B. avium 197N expresses low levels of cellulose in air–liquid interface biofilms and that biofilm strength and attachment levels could be increased by elevating c-di-GMP levels like the pseudomonads, but cellulose was not required for biofilm formation itself. The finding that B. avium 197N is capable of producing cellulose from a highly-conserved, but relatively uncommon bcs operon raises the question of what functional role this modified polymer plays during the infection of the upper respiratory tract or survival between hosts, and what environmental signals control its production.",
author = "Kimberley McLaughlin and Folorunso, {Ayorinde O.} and Deeni, {Yusuf Y.} and Dona Foster and Oksana Gorbatiuk and Hapca, {Simona M.} and Corinna Immoor and Anna Koza and Ibrahim Mohammed and Olena Moshynets and Sergii Rogalsky and Kamil Zawadzki and Spiers, {Andrew J.}",
year = "2017",
month = "6",
doi = "10.1016/j.resmic.2017.01.002",
language = "English",
volume = "168",
pages = "419–430",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier Masson SAS",
number = "5",

}

McLaughlin, K, Folorunso, AO, Deeni, YY, Foster, D, Gorbatiuk, O, Hapca, SM, Immoor, C, Koza, A, Mohammed, I, Moshynets, O, Rogalsky, S, Zawadzki, K & Spiers, AJ 2017, 'Biofilm formation and cellulose expression by Bordetella avium 197N, the causative agent of bordetellosis in birds and an opportunistic respiratory pathogen in humans' Research in Microbiology, vol. 168, no. 5, pp. 419–430. https://doi.org/10.1016/j.resmic.2017.01.002

Biofilm formation and cellulose expression by Bordetella avium 197N, the causative agent of bordetellosis in birds and an opportunistic respiratory pathogen in humans. / McLaughlin, Kimberley; Folorunso, Ayorinde O.; Deeni, Yusuf Y.; Foster, Dona; Gorbatiuk, Oksana; Hapca, Simona M.; Immoor, Corinna; Koza, Anna; Mohammed, Ibrahim; Moshynets, Olena; Rogalsky, Sergii; Zawadzki, Kamil; Spiers, Andrew J.

In: Research in Microbiology, Vol. 168, No. 5, 06.2017, p. 419–430.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biofilm formation and cellulose expression by Bordetella avium 197N, the causative agent of bordetellosis in birds and an opportunistic respiratory pathogen in humans

AU - McLaughlin, Kimberley

AU - Folorunso, Ayorinde O.

AU - Deeni, Yusuf Y.

AU - Foster, Dona

AU - Gorbatiuk, Oksana

AU - Hapca, Simona M.

AU - Immoor, Corinna

AU - Koza, Anna

AU - Mohammed, Ibrahim

AU - Moshynets, Olena

AU - Rogalsky, Sergii

AU - Zawadzki, Kamil

AU - Spiers, Andrew J.

PY - 2017/6

Y1 - 2017/6

N2 - Although bacterial cellulose synthase (bcs) operons are widespread within the Proteobacteria phylum, subunits required for the partial-acetylation of the polymer appear to be restricted to a few γ-group soil, plant-associated and phytopathogenic pseudomonads, including Pseudomonas fluorescens SBW25 and several Pseudomonas syringae pathovars. However, a bcs operon with acetylation subunits has also been annotated in the unrelated β-group respiratory pathogen, Bordetella avium 197N. Our comparison of subunit protein sequences and GC content analyses confirms the close similarity between the B. avium 197N and pseudomonad operons and suggests that, in both cases, the cellulose synthase and acetylation subunits were acquired as a single unit. Using static liquid microcosms, we can confirm that B. avium 197N expresses low levels of cellulose in air–liquid interface biofilms and that biofilm strength and attachment levels could be increased by elevating c-di-GMP levels like the pseudomonads, but cellulose was not required for biofilm formation itself. The finding that B. avium 197N is capable of producing cellulose from a highly-conserved, but relatively uncommon bcs operon raises the question of what functional role this modified polymer plays during the infection of the upper respiratory tract or survival between hosts, and what environmental signals control its production.

AB - Although bacterial cellulose synthase (bcs) operons are widespread within the Proteobacteria phylum, subunits required for the partial-acetylation of the polymer appear to be restricted to a few γ-group soil, plant-associated and phytopathogenic pseudomonads, including Pseudomonas fluorescens SBW25 and several Pseudomonas syringae pathovars. However, a bcs operon with acetylation subunits has also been annotated in the unrelated β-group respiratory pathogen, Bordetella avium 197N. Our comparison of subunit protein sequences and GC content analyses confirms the close similarity between the B. avium 197N and pseudomonad operons and suggests that, in both cases, the cellulose synthase and acetylation subunits were acquired as a single unit. Using static liquid microcosms, we can confirm that B. avium 197N expresses low levels of cellulose in air–liquid interface biofilms and that biofilm strength and attachment levels could be increased by elevating c-di-GMP levels like the pseudomonads, but cellulose was not required for biofilm formation itself. The finding that B. avium 197N is capable of producing cellulose from a highly-conserved, but relatively uncommon bcs operon raises the question of what functional role this modified polymer plays during the infection of the upper respiratory tract or survival between hosts, and what environmental signals control its production.

U2 - 10.1016/j.resmic.2017.01.002

DO - 10.1016/j.resmic.2017.01.002

M3 - Article

VL - 168

SP - 419

EP - 430

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

IS - 5

ER -