Biological evaluation of new nickel(II) metallates: synthesis, DNA/protein binding and mitochondrial mediated apoptosis in human lung cancer cells (A549) via ROS hypergeneration and depletion of cellular antioxidant pool

P. Kalaivani, S. Saranya, P. Poornima, R. Prabhakaran*, F. Dallemer, V. Vijaya Padma, K. Natarajan

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A series of novel nickel(11) thiosemicarbazone complexes(1-4) have been prepared and characterized by various spectral, analytical techniques and X-ray crystallography. Further, their efficacy to interact with CT-DNA/BSA has been explored. From the binding studies, it is inferred that complex 4 found to be more active than other complexes. The complexes bound with CT-DNA by intercalation mode. Moreover, static quenching was observed for their interaction with BSA. The new complexes were tested for their in vitro cytotoxicity against human lung adenocarcinoma (A549) cell line. The results showed that the new complexes exhibited significant degree of cytotoxicity at given experimental condition. Further, the results of LDH and NO release supported the cytotoxic nature of the complexes. The observed cytotoxicity of the complexes may be routed through ROS-hypergeneration and lipid-peroxidation with subsequent depletion of cellular antioxidant pool (GSH, SOD, CAT, GPx and GST) resulted in the reduction of mitochondrial-membrane potential, caspase-3 activation and DNA fragmentation. Thus, the data from the present study disclose that the complexes could induce apoptosis in A549 cells through mitochondrial mediated fashion and inhibited the migration of lung cancer cells and by metastasis. (C) 2014 Elsevier Masson SAS. All rights reserved.

Original languageEnglish
Pages (from-to)584-599
Number of pages16
JournalEuropean Journal of Medicinal Chemistry
Volume82
Early online date2 Jun 2014
DOIs
Publication statusPublished - 23 Jul 2014
Externally publishedYes

Cite this

@article{0c2a369ade8c481ebdc665206f65d358,
title = "Biological evaluation of new nickel(II) metallates: synthesis, DNA/protein binding and mitochondrial mediated apoptosis in human lung cancer cells (A549) via ROS hypergeneration and depletion of cellular antioxidant pool",
abstract = "A series of novel nickel(11) thiosemicarbazone complexes(1-4) have been prepared and characterized by various spectral, analytical techniques and X-ray crystallography. Further, their efficacy to interact with CT-DNA/BSA has been explored. From the binding studies, it is inferred that complex 4 found to be more active than other complexes. The complexes bound with CT-DNA by intercalation mode. Moreover, static quenching was observed for their interaction with BSA. The new complexes were tested for their in vitro cytotoxicity against human lung adenocarcinoma (A549) cell line. The results showed that the new complexes exhibited significant degree of cytotoxicity at given experimental condition. Further, the results of LDH and NO release supported the cytotoxic nature of the complexes. The observed cytotoxicity of the complexes may be routed through ROS-hypergeneration and lipid-peroxidation with subsequent depletion of cellular antioxidant pool (GSH, SOD, CAT, GPx and GST) resulted in the reduction of mitochondrial-membrane potential, caspase-3 activation and DNA fragmentation. Thus, the data from the present study disclose that the complexes could induce apoptosis in A549 cells through mitochondrial mediated fashion and inhibited the migration of lung cancer cells and by metastasis. (C) 2014 Elsevier Masson SAS. All rights reserved.",
author = "P. Kalaivani and S. Saranya and P. Poornima and R. Prabhakaran and F. Dallemer and Padma, {V. Vijaya} and K. Natarajan",
year = "2014",
month = "7",
day = "23",
doi = "10.1016/j.ejmech.2014.05.075",
language = "English",
volume = "82",
pages = "584--599",
journal = "European Journal of Medicinal Chemistry",
issn = "0223-5234",
publisher = "ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER",

}

Biological evaluation of new nickel(II) metallates : synthesis, DNA/protein binding and mitochondrial mediated apoptosis in human lung cancer cells (A549) via ROS hypergeneration and depletion of cellular antioxidant pool. / Kalaivani, P.; Saranya, S.; Poornima, P.; Prabhakaran, R.; Dallemer, F.; Padma, V. Vijaya; Natarajan, K.

In: European Journal of Medicinal Chemistry, Vol. 82, 23.07.2014, p. 584-599.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biological evaluation of new nickel(II) metallates

T2 - synthesis, DNA/protein binding and mitochondrial mediated apoptosis in human lung cancer cells (A549) via ROS hypergeneration and depletion of cellular antioxidant pool

AU - Kalaivani, P.

AU - Saranya, S.

AU - Poornima, P.

AU - Prabhakaran, R.

AU - Dallemer, F.

AU - Padma, V. Vijaya

AU - Natarajan, K.

PY - 2014/7/23

Y1 - 2014/7/23

N2 - A series of novel nickel(11) thiosemicarbazone complexes(1-4) have been prepared and characterized by various spectral, analytical techniques and X-ray crystallography. Further, their efficacy to interact with CT-DNA/BSA has been explored. From the binding studies, it is inferred that complex 4 found to be more active than other complexes. The complexes bound with CT-DNA by intercalation mode. Moreover, static quenching was observed for their interaction with BSA. The new complexes were tested for their in vitro cytotoxicity against human lung adenocarcinoma (A549) cell line. The results showed that the new complexes exhibited significant degree of cytotoxicity at given experimental condition. Further, the results of LDH and NO release supported the cytotoxic nature of the complexes. The observed cytotoxicity of the complexes may be routed through ROS-hypergeneration and lipid-peroxidation with subsequent depletion of cellular antioxidant pool (GSH, SOD, CAT, GPx and GST) resulted in the reduction of mitochondrial-membrane potential, caspase-3 activation and DNA fragmentation. Thus, the data from the present study disclose that the complexes could induce apoptosis in A549 cells through mitochondrial mediated fashion and inhibited the migration of lung cancer cells and by metastasis. (C) 2014 Elsevier Masson SAS. All rights reserved.

AB - A series of novel nickel(11) thiosemicarbazone complexes(1-4) have been prepared and characterized by various spectral, analytical techniques and X-ray crystallography. Further, their efficacy to interact with CT-DNA/BSA has been explored. From the binding studies, it is inferred that complex 4 found to be more active than other complexes. The complexes bound with CT-DNA by intercalation mode. Moreover, static quenching was observed for their interaction with BSA. The new complexes were tested for their in vitro cytotoxicity against human lung adenocarcinoma (A549) cell line. The results showed that the new complexes exhibited significant degree of cytotoxicity at given experimental condition. Further, the results of LDH and NO release supported the cytotoxic nature of the complexes. The observed cytotoxicity of the complexes may be routed through ROS-hypergeneration and lipid-peroxidation with subsequent depletion of cellular antioxidant pool (GSH, SOD, CAT, GPx and GST) resulted in the reduction of mitochondrial-membrane potential, caspase-3 activation and DNA fragmentation. Thus, the data from the present study disclose that the complexes could induce apoptosis in A549 cells through mitochondrial mediated fashion and inhibited the migration of lung cancer cells and by metastasis. (C) 2014 Elsevier Masson SAS. All rights reserved.

U2 - 10.1016/j.ejmech.2014.05.075

DO - 10.1016/j.ejmech.2014.05.075

M3 - Article

VL - 82

SP - 584

EP - 599

JO - European Journal of Medicinal Chemistry

JF - European Journal of Medicinal Chemistry

SN - 0223-5234

ER -