Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery

Huihui Yang, David H. Bremner, Lei Tao, He-yu Li, Juan Hu, Li-min Zhu

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95% and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.
Original languageEnglish
Pages (from-to)72-78
Number of pages7
JournalCarbohydrate Polymers
Volume135
Early online date22 Aug 2015
DOIs
Publication statusPublished - 1 Jan 2016

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Hyaluronic acid
Graphite
Hyaluronic Acid
Drug delivery
Chitosan
Oxides
Graphene
Cells
Pharmaceutical Preparations
Doxorubicin
Tumors
Drug Carriers
Fluorescein
carboxymethyl-chitosan

Cite this

Yang, Huihui ; Bremner, David H. ; Tao, Lei ; Li, He-yu ; Hu, Juan ; Zhu, Li-min. / Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery. In: Carbohydrate Polymers. 2016 ; Vol. 135. pp. 72-78.
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abstract = "In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95{\%} and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.",
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Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery. / Yang, Huihui; Bremner, David H.; Tao, Lei; Li, He-yu; Hu, Juan; Zhu, Li-min.

In: Carbohydrate Polymers, Vol. 135, 01.01.2016, p. 72-78.

Research output: Contribution to journalArticle

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AU - Bremner, David H.

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AU - Zhu, Li-min

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AB - In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95% and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.

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