Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery

Huihui Yang, David H. Bremner, Lei Tao, He-yu Li, Juan Hu, Li-min Zhu

    Research output: Contribution to journalArticle

    79 Citations (Scopus)

    Abstract

    In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95% and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.
    Original languageEnglish
    Pages (from-to)72-78
    Number of pages7
    JournalCarbohydrate Polymers
    Volume135
    Early online date22 Aug 2015
    DOIs
    Publication statusPublished - 1 Jan 2016

    Fingerprint

    Hyaluronic acid
    Graphite
    Hyaluronic Acid
    Drug delivery
    Chitosan
    Oxides
    Graphene
    Cells
    Pharmaceutical Preparations
    Doxorubicin
    Tumors
    Drug Carriers
    Fluorescein
    carboxymethyl-chitosan

    Cite this

    Yang, Huihui ; Bremner, David H. ; Tao, Lei ; Li, He-yu ; Hu, Juan ; Zhu, Li-min. / Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery. In: Carbohydrate Polymers. 2016 ; Vol. 135. pp. 72-78.
    @article{79978b3d07744376b4ccf6cb97b010b5,
    title = "Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery",
    abstract = "In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95{\%} and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.",
    author = "Huihui Yang and Bremner, {David H.} and Lei Tao and He-yu Li and Juan Hu and Li-min Zhu",
    year = "2016",
    month = "1",
    day = "1",
    doi = "10.1016/j.carbpol.2015.08.058",
    language = "English",
    volume = "135",
    pages = "72--78",
    journal = "Carbohydrate Polymers",
    issn = "0144-8617",
    publisher = "Elsevier Limited",

    }

    Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery. / Yang, Huihui; Bremner, David H.; Tao, Lei; Li, He-yu; Hu, Juan; Zhu, Li-min.

    In: Carbohydrate Polymers, Vol. 135, 01.01.2016, p. 72-78.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Carboxymethyl chitosan-mediated synthesis of hyaluronic acid-targeted graphene oxide for cancer drug delivery

    AU - Yang, Huihui

    AU - Bremner, David H.

    AU - Tao, Lei

    AU - Li, He-yu

    AU - Hu, Juan

    AU - Zhu, Li-min

    PY - 2016/1/1

    Y1 - 2016/1/1

    N2 - In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95% and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.

    AB - In order to enhance the efficiency and specificity of anticancer drug delivery and realize intelligently controlled release, a new drug carrier was developed. Graphene oxide (GO) was first modified with carboxymethyl chitosan (CMC), followed by conjugation of hyaluronic acid (HA) and fluorescein isothiocyanate (FI). The resulting GO–CMC–FI–HA conjugate was characterized and used as a carrier to encapsulate the anticancer drug doxorubicin (DOX) to study in vitro release behavior. The drug loading capacity is as high as 95% and the drug release rate under tumor cell microenvironment of pH 5.8 is significantly higher than that under physiological conditions of pH 7.4. Cell uptake studies show that the GO–CMC–FI–HA/DOX complex can specifically target cancer cells, which are over-expressing CD44 receptors and effectively inhibit their growth. The above results suggest that the functionalized graphene-based material has potential applications for targeted delivery and controlled release of anticancer drugs.

    U2 - 10.1016/j.carbpol.2015.08.058

    DO - 10.1016/j.carbpol.2015.08.058

    M3 - Article

    VL - 135

    SP - 72

    EP - 78

    JO - Carbohydrate Polymers

    JF - Carbohydrate Polymers

    SN - 0144-8617

    ER -