Cell surface properties and flocculation behaviour of industrial strains of Saccharomyces cerevisiae

Ashima Nayyar, Graeme Walker, Elisabetta Canetta, Forbes Wardrop, Ashok K. Adya

    Research output: Contribution to journalArticle

    Abstract

    Cellular adhesion properties of yeasts depend on the characteristics of the outer layer of the cell wall. In this study, the flocculation behaviour of four industrial strains of Saccharomyces cerevisiae used for production of beer, champagne, wine and fuel alcohol was evaluated; their flocculation abilities being, 42.5, 14.8, 13.8 and 11.6%, respectively. The brewing yeast strain was found to be the most flocculent. Very little flocculation was observed during the lag and logarithmic phases of growth (1-15%), while during the early and late stationary phases, different strains exhibited variable flocculation patterns. Cell surface hydrophobicity [assayed using hydrophobicity microsphere assay (HMA) and microbial adhesion to solvent (MATS) techniques] and surface charge (assayed by Alcian blue dye retention) played important roles in dictating flocculation behaviour in different yeast strains, as did the yeast growth phase. Percentage hydrophobicity index (HI) and percentage hydrophobicity of the four strains followed, respectively the same order, viz: Beer (66.6, 21.5) > Champagne (33, 10.5) > fuel alcohol (22.4, 7.4) > wine (20.5, 2.7). Our findings provide new insight into yeast cell surface properties and how these relate to behavioural characteristics of yeasts employed in industrial fermentations.
    Original languageEnglish
    Pages (from-to)64-72
    Number of pages9
    JournalInternational Journal of Applied Microbiology and Biotechnology Research
    Volume2
    Issue number6
    Publication statusPublished - Nov 2014

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    Flocculation
    Surface Properties
    Saccharomyces cerevisiae
    Yeasts
    Hydrophobic and Hydrophilic Interactions
    Wine
    Alcohols
    Alcian Blue
    Growth
    Microspheres
    Cell Wall
    Fermentation
    Coloring Agents

    Cite this

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    title = "Cell surface properties and flocculation behaviour of industrial strains of Saccharomyces cerevisiae",
    abstract = "Cellular adhesion properties of yeasts depend on the characteristics of the outer layer of the cell wall. In this study, the flocculation behaviour of four industrial strains of Saccharomyces cerevisiae used for production of beer, champagne, wine and fuel alcohol was evaluated; their flocculation abilities being, 42.5, 14.8, 13.8 and 11.6{\%}, respectively. The brewing yeast strain was found to be the most flocculent. Very little flocculation was observed during the lag and logarithmic phases of growth (1-15{\%}), while during the early and late stationary phases, different strains exhibited variable flocculation patterns. Cell surface hydrophobicity [assayed using hydrophobicity microsphere assay (HMA) and microbial adhesion to solvent (MATS) techniques] and surface charge (assayed by Alcian blue dye retention) played important roles in dictating flocculation behaviour in different yeast strains, as did the yeast growth phase. Percentage hydrophobicity index (HI) and percentage hydrophobicity of the four strains followed, respectively the same order, viz: Beer (66.6, 21.5) > Champagne (33, 10.5) > fuel alcohol (22.4, 7.4) > wine (20.5, 2.7). Our findings provide new insight into yeast cell surface properties and how these relate to behavioural characteristics of yeasts employed in industrial fermentations.",
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    Cell surface properties and flocculation behaviour of industrial strains of Saccharomyces cerevisiae. / Nayyar, Ashima; Walker, Graeme; Canetta, Elisabetta; Wardrop, Forbes; Adya, Ashok K.

    In: International Journal of Applied Microbiology and Biotechnology Research, Vol. 2, No. 6, 11.2014, p. 64-72.

    Research output: Contribution to journalArticle

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    AU - Nayyar, Ashima

    AU - Walker, Graeme

    AU - Canetta, Elisabetta

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    AU - Adya, Ashok K.

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