Changes in calcium and magnesium levels during heat-shock synchronized cell division in Tetrahymena

Graeme M. Walker, Erik Zeuthen

Research output: Contribution to journalArticle

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Abstract

Calcium and magnesium contents were measured in cells of Tetrahymena pyriformis induced to divide synchronously by a multi-heat-shock procedure. During free-running synchronized cell division in complex proteose peptone medium, significant peaks of both calcium and magnesium were observed at points in the cell cycle just prior to division. No such peaks were detected in cells dividing asynchronously in proteose peptone. When synchronized cell division was followed after transfer to an inorganic medium, cell calcium and magnesium levels were observed to decrease in relation to the corresponding cell number increase, indicating that in concentration terms, calcium and magnesium remain fairly constant. This latter result suggests that neither calcium nor magnesium influxes act as triggers for cell division in Tetrahymena and that the fluctuations of these metals seen during the synchronized division cycle in complex medium represent an effect rather than a cause.
Original languageEnglish
Pages (from-to)487-490
Number of pages4
JournalExperimental Cell Research
Volume127
Issue number2
DOIs
StatePublished - Jun 1980

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Tetrahymena
Cell Division
Magnesium
Hot Temperature
Calcium
Peptones
Tetrahymena pyriformis
Cell Cycle
Cell Count
Metals

Cite this

Walker, Graeme M.; Zeuthen, Erik / Changes in calcium and magnesium levels during heat-shock synchronized cell division in Tetrahymena.

In: Experimental Cell Research, Vol. 127, No. 2, 06.1980, p. 487-490.

Research output: Contribution to journalArticle

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abstract = "Calcium and magnesium contents were measured in cells of Tetrahymena pyriformis induced to divide synchronously by a multi-heat-shock procedure. During free-running synchronized cell division in complex proteose peptone medium, significant peaks of both calcium and magnesium were observed at points in the cell cycle just prior to division. No such peaks were detected in cells dividing asynchronously in proteose peptone. When synchronized cell division was followed after transfer to an inorganic medium, cell calcium and magnesium levels were observed to decrease in relation to the corresponding cell number increase, indicating that in concentration terms, calcium and magnesium remain fairly constant. This latter result suggests that neither calcium nor magnesium influxes act as triggers for cell division in Tetrahymena and that the fluctuations of these metals seen during the synchronized division cycle in complex medium represent an effect rather than a cause.",
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Changes in calcium and magnesium levels during heat-shock synchronized cell division in Tetrahymena. / Walker, Graeme M.; Zeuthen, Erik.

In: Experimental Cell Research, Vol. 127, No. 2, 06.1980, p. 487-490.

Research output: Contribution to journalArticle

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