In adolescent soccer, 23% of the distance covers happens at speeds above onset of blood lactate accumulation which suggests that lactate kinetics may be important for soccer performance. We sought to determine the effectiveness of sprint interval training (SIT) on changing performance and lactate kinetics in adolescent soccer players. Thirteen elite soccer academy players (age 15 ± 0.5y) underwent baseline testing (0-10m and 10-20m sprint performance, Wingate anaerobic Test (WaNT) with blood lactate measurements and incremental VO2 peak test) before being allocated to control or SIT group. The control group maintained training whilst the HIT group carried out twice-weekly all-out effort cycle sprints consisting of 6 x 10sec sprint with 80sec recovery. There were significant time x group interactions for 10- 20m sprint time (Control pre: 1.32 ± 0.07s post: 1.35 ± 0.08s; SIT pre: 1.29 ± 0.04s post: 1.25 ± 0.04s; p=0.01), Peak Power (Control pre: 13.1 ± 1.3W.kg-1 post: 13.2 ± 1.47 W.kg-1; SIT pre: 12.4 ± 1.3 W.kg-1 post: 15.3 ± 0.7W.kg-1; p=0.01) and time to exhaustion (Control pre: 596 ± 62s post: 562 ± 85s; SIT pre: 655 ± 54s post: 688 ± 55s; p=0.001). The changes in performance were significantly correlated to changes in lactate kinetics (power: r=0.55; 10-20m speed: r=-0.54; time to exhaustion: r=0.55). Therefore, cycle based SIT is an effective training paradigm for elite adolescent soccer players and the improvements in performance are associated with changes in lactate kinetics.