Characterising grey seals' blubber structure

Research output: Contribution to conferenceAbstract

Abstract

Accurate regulation of energy stores is linked to reproductive success and survival. Fat deposition and mobilisation has been associated with changes in adipocyte size and number, extracellular matrix deposition, vascularity among other adaptations in other mammals. How seals’ blubber adjusts with rapid body composition changes is unknown. Biopsy samples from mother and pup pairs (n = 6) were collected at early, late and pup’s early weaning lactation. Samples were processed and stained with Masson’s Trichrome. Biopsies were divided in three sections: inner, middle and outer blubber. Five microphotographs of each section were taken to be averaged. Adipocyte size/ number, collagen area and blood vessels were measured with Image J and Adiposoft plugin. Linear mixed models were used to investigate variation in relation to weight, depth and metabolic state with in each age class and comparison between fasting mums and pups. Preliminary results showed that, consistently with other mammals, adipocyte size increased with weight as adipocyte number per picture is reduced. Collagen area decreased in late lactation and weaning in pups, but not mum. Vascularity was not significantly associated to any measured variable. According to preliminary results, grey seals’ blubber structure is not stratified and only adipocytes vary with weight changes.
Original languageEnglish
Pages5-5
Number of pages1
Publication statusPublished - 10 Jan 2020
Event14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference - Galway-Mayo Institute of Technology, Galway, Ireland
Duration: 10 Jan 202011 Jan 2020
Conference number: 14th
https://synergy.st-andrews.ac.uk/ukrsc/conference-2020/

Conference

Conference14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference
Abbreviated titleUKIRSC 20
CountryIreland
CityGalway
Period10/01/2011/01/20
Internet address

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blubber
adipocytes
seals
pups
collagen
biopsy
mammals
early weaning
Chrysanthemum morifolium
late lactation
extracellular matrix
blood vessels
body composition
age structure
fasting
weaning
lactation
sampling
energy
lipids

Cite this

Oller, L., Rocha, J., Dagleish, M., & Bennett, K. (2020). Characterising grey seals' blubber structure. 5-5. Abstract from 14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference, Galway, Ireland.
Oller, Laura ; Rocha, Joel ; Dagleish, Mark ; Bennett, Kimberley. / Characterising grey seals' blubber structure. Abstract from 14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference, Galway, Ireland.1 p.
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title = "Characterising grey seals' blubber structure",
abstract = "Accurate regulation of energy stores is linked to reproductive success and survival. Fat deposition and mobilisation has been associated with changes in adipocyte size and number, extracellular matrix deposition, vascularity among other adaptations in other mammals. How seals’ blubber adjusts with rapid body composition changes is unknown. Biopsy samples from mother and pup pairs (n = 6) were collected at early, late and pup’s early weaning lactation. Samples were processed and stained with Masson’s Trichrome. Biopsies were divided in three sections: inner, middle and outer blubber. Five microphotographs of each section were taken to be averaged. Adipocyte size/ number, collagen area and blood vessels were measured with Image J and Adiposoft plugin. Linear mixed models were used to investigate variation in relation to weight, depth and metabolic state with in each age class and comparison between fasting mums and pups. Preliminary results showed that, consistently with other mammals, adipocyte size increased with weight as adipocyte number per picture is reduced. Collagen area decreased in late lactation and weaning in pups, but not mum. Vascularity was not significantly associated to any measured variable. According to preliminary results, grey seals’ blubber structure is not stratified and only adipocytes vary with weight changes.",
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Oller, L, Rocha, J, Dagleish, M & Bennett, K 2020, 'Characterising grey seals' blubber structure', 14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference, Galway, Ireland, 10/01/20 - 11/01/20 pp. 5-5.

Characterising grey seals' blubber structure. / Oller, Laura; Rocha, Joel; Dagleish, Mark; Bennett, Kimberley.

2020. 5-5 Abstract from 14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference, Galway, Ireland.

Research output: Contribution to conferenceAbstract

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AU - Rocha, Joel

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AU - Bennett, Kimberley

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N2 - Accurate regulation of energy stores is linked to reproductive success and survival. Fat deposition and mobilisation has been associated with changes in adipocyte size and number, extracellular matrix deposition, vascularity among other adaptations in other mammals. How seals’ blubber adjusts with rapid body composition changes is unknown. Biopsy samples from mother and pup pairs (n = 6) were collected at early, late and pup’s early weaning lactation. Samples were processed and stained with Masson’s Trichrome. Biopsies were divided in three sections: inner, middle and outer blubber. Five microphotographs of each section were taken to be averaged. Adipocyte size/ number, collagen area and blood vessels were measured with Image J and Adiposoft plugin. Linear mixed models were used to investigate variation in relation to weight, depth and metabolic state with in each age class and comparison between fasting mums and pups. Preliminary results showed that, consistently with other mammals, adipocyte size increased with weight as adipocyte number per picture is reduced. Collagen area decreased in late lactation and weaning in pups, but not mum. Vascularity was not significantly associated to any measured variable. According to preliminary results, grey seals’ blubber structure is not stratified and only adipocytes vary with weight changes.

AB - Accurate regulation of energy stores is linked to reproductive success and survival. Fat deposition and mobilisation has been associated with changes in adipocyte size and number, extracellular matrix deposition, vascularity among other adaptations in other mammals. How seals’ blubber adjusts with rapid body composition changes is unknown. Biopsy samples from mother and pup pairs (n = 6) were collected at early, late and pup’s early weaning lactation. Samples were processed and stained with Masson’s Trichrome. Biopsies were divided in three sections: inner, middle and outer blubber. Five microphotographs of each section were taken to be averaged. Adipocyte size/ number, collagen area and blood vessels were measured with Image J and Adiposoft plugin. Linear mixed models were used to investigate variation in relation to weight, depth and metabolic state with in each age class and comparison between fasting mums and pups. Preliminary results showed that, consistently with other mammals, adipocyte size increased with weight as adipocyte number per picture is reduced. Collagen area decreased in late lactation and weaning in pups, but not mum. Vascularity was not significantly associated to any measured variable. According to preliminary results, grey seals’ blubber structure is not stratified and only adipocytes vary with weight changes.

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Oller L, Rocha J, Dagleish M, Bennett K. Characterising grey seals' blubber structure. 2020. Abstract from 14th Annual UK and Ireland Society for Marine Mammalogy Student Chapter Conference, Galway, Ireland.