Characterization of Piriformospora indica culture filtrate

Ashok K. Adya, Anju Gautam, Lixin Zhang, Ajit Varma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Sustainable crop production under biotic and abiotic stresses is a worldwide agricultural, eco-environmental, and economic problem that requires careful strategy, planning, research, and action to meet the needs of world’s growing population. One possible route to overcome these problems is to develop stress-tolerant crops through plant breeding. Such approaches have been successful for some plant species but not all the crops. Additionally, most of these approaches remain beyond the economic means of developing nations. An alternative route is to exploit mutualistic symbiosis between plants and arbuscular mycorrhizal fungi (AMF) to stimulate and promote plant growth. Piriformospora indica, belonging to the Sebacinales in Basidiomycota, is a newly described root endophyte (Varma (1998) Mycorrhiza manual. Springer, Heidelberg; Verma et al. Mycologia 90:895–909, 1998) with AMF-like characteristics, and it can be axenically grown unlike AMF that are obligate endosymbionts. Like fungal biomass, the culture filtrate also facilitates early seed germination and plant growth. Proper characterization of Piriformospora indica culture filtrate is an essential prerequisite to understand the underlying and still unknown mechanism(s) by which this fungus or its culture filtrate acts as a plant growth stimulator, promoter, and bioprotector. This chapter is devoted to develop a detailed protocol for the characterization and identification of P. indica culture filtrate.
Original languageEnglish
Title of host publicationPiriformospora indica
Subtitle of host publicationSebacinales and their biotechnological applications
EditorsAjit Varma, Gerhard Kost, Ralf Oelmüller
Place of PublicationBerlin
PublisherSpringer-Verlag
Pages345-375
Number of pages31
ISBN (Electronic)9783642338021
ISBN (Print)9783642338014
DOIs
Publication statusPublished - 2 Jan 2013

Publication series

NameSoil Biology
PublisherSpringer-Verlag
Volume33
ISSN (Print)1613-3382

Fingerprint

culture filtrates
mycorrhizal fungi
plant growth
plant breeding
Sebacinales
research planning
economics
endosymbionts
sustainable agriculture
crops
biotic stress
Spermatophytina
endophytes
Basidiomycota
mycorrhizae
symbiosis
abiotic stress
developing countries
seed germination
promoter regions

Cite this

Adya, A. K., Gautam, A., Zhang, L., & Varma, A. (2013). Characterization of Piriformospora indica culture filtrate. In A. Varma, G. Kost, & R. Oelmüller (Eds.), Piriformospora indica: Sebacinales and their biotechnological applications (pp. 345-375). (Soil Biology; Vol. 33). Berlin: Springer-Verlag. https://doi.org/10.1007/978-3-642-33802-1_21
Adya, Ashok K. ; Gautam, Anju ; Zhang, Lixin ; Varma, Ajit. / Characterization of Piriformospora indica culture filtrate. Piriformospora indica: Sebacinales and their biotechnological applications. editor / Ajit Varma ; Gerhard Kost ; Ralf Oelmüller. Berlin : Springer-Verlag, 2013. pp. 345-375 (Soil Biology).
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Adya, AK, Gautam, A, Zhang, L & Varma, A 2013, Characterization of Piriformospora indica culture filtrate. in A Varma, G Kost & R Oelmüller (eds), Piriformospora indica: Sebacinales and their biotechnological applications. Soil Biology, vol. 33, Springer-Verlag, Berlin, pp. 345-375. https://doi.org/10.1007/978-3-642-33802-1_21

Characterization of Piriformospora indica culture filtrate. / Adya, Ashok K.; Gautam, Anju; Zhang, Lixin; Varma, Ajit.

Piriformospora indica: Sebacinales and their biotechnological applications. ed. / Ajit Varma; Gerhard Kost; Ralf Oelmüller. Berlin : Springer-Verlag, 2013. p. 345-375 (Soil Biology; Vol. 33).

Research output: Chapter in Book/Report/Conference proceedingChapter

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N2 - Sustainable crop production under biotic and abiotic stresses is a worldwide agricultural, eco-environmental, and economic problem that requires careful strategy, planning, research, and action to meet the needs of world’s growing population. One possible route to overcome these problems is to develop stress-tolerant crops through plant breeding. Such approaches have been successful for some plant species but not all the crops. Additionally, most of these approaches remain beyond the economic means of developing nations. An alternative route is to exploit mutualistic symbiosis between plants and arbuscular mycorrhizal fungi (AMF) to stimulate and promote plant growth. Piriformospora indica, belonging to the Sebacinales in Basidiomycota, is a newly described root endophyte (Varma (1998) Mycorrhiza manual. Springer, Heidelberg; Verma et al. Mycologia 90:895–909, 1998) with AMF-like characteristics, and it can be axenically grown unlike AMF that are obligate endosymbionts. Like fungal biomass, the culture filtrate also facilitates early seed germination and plant growth. Proper characterization of Piriformospora indica culture filtrate is an essential prerequisite to understand the underlying and still unknown mechanism(s) by which this fungus or its culture filtrate acts as a plant growth stimulator, promoter, and bioprotector. This chapter is devoted to develop a detailed protocol for the characterization and identification of P. indica culture filtrate.

AB - Sustainable crop production under biotic and abiotic stresses is a worldwide agricultural, eco-environmental, and economic problem that requires careful strategy, planning, research, and action to meet the needs of world’s growing population. One possible route to overcome these problems is to develop stress-tolerant crops through plant breeding. Such approaches have been successful for some plant species but not all the crops. Additionally, most of these approaches remain beyond the economic means of developing nations. An alternative route is to exploit mutualistic symbiosis between plants and arbuscular mycorrhizal fungi (AMF) to stimulate and promote plant growth. Piriformospora indica, belonging to the Sebacinales in Basidiomycota, is a newly described root endophyte (Varma (1998) Mycorrhiza manual. Springer, Heidelberg; Verma et al. Mycologia 90:895–909, 1998) with AMF-like characteristics, and it can be axenically grown unlike AMF that are obligate endosymbionts. Like fungal biomass, the culture filtrate also facilitates early seed germination and plant growth. Proper characterization of Piriformospora indica culture filtrate is an essential prerequisite to understand the underlying and still unknown mechanism(s) by which this fungus or its culture filtrate acts as a plant growth stimulator, promoter, and bioprotector. This chapter is devoted to develop a detailed protocol for the characterization and identification of P. indica culture filtrate.

U2 - 10.1007/978-3-642-33802-1_21

DO - 10.1007/978-3-642-33802-1_21

M3 - Chapter

SN - 9783642338014

T3 - Soil Biology

SP - 345

EP - 375

BT - Piriformospora indica

A2 - Varma, Ajit

A2 - Kost, Gerhard

A2 - Oelmüller, Ralf

PB - Springer-Verlag

CY - Berlin

ER -

Adya AK, Gautam A, Zhang L, Varma A. Characterization of Piriformospora indica culture filtrate. In Varma A, Kost G, Oelmüller R, editors, Piriformospora indica: Sebacinales and their biotechnological applications. Berlin: Springer-Verlag. 2013. p. 345-375. (Soil Biology). https://doi.org/10.1007/978-3-642-33802-1_21