Cloning differentially regulated genes from chondrocytes using agarose gel differential display

David Jefferies, M. Botman, Colin Farquharson, Douglas Lester, C. C. Whitehead, B. H. Thorp, Brian Houston

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.

    Original languageEnglish
    Pages (from-to)237-241
    Number of pages5
    JournalBiochimica et Biophysica Acta - General Subjects
    Volume1396
    Issue number3
    DOIs
    Publication statusPublished - 13 Mar 1998

    Fingerprint

    Cloning
    Chondrocytes
    Sepharose
    Organism Cloning
    Genes
    Gels
    Display devices
    RNA
    Clone Cells
    Northern Blotting
    Hypertrophy
    Tissue

    Cite this

    Jefferies, D., Botman, M., Farquharson, C., Lester, D., Whitehead, C. C., Thorp, B. H., & Houston, B. (1998). Cloning differentially regulated genes from chondrocytes using agarose gel differential display. Biochimica et Biophysica Acta - General Subjects, 1396(3), 237-241. https://doi.org/10.1016/S0167-4781(97)00234-0
    Jefferies, David ; Botman, M. ; Farquharson, Colin ; Lester, Douglas ; Whitehead, C. C. ; Thorp, B. H. ; Houston, Brian. / Cloning differentially regulated genes from chondrocytes using agarose gel differential display. In: Biochimica et Biophysica Acta - General Subjects. 1998 ; Vol. 1396, No. 3. pp. 237-241.
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    abstract = "The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.",
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    Jefferies, D, Botman, M, Farquharson, C, Lester, D, Whitehead, CC, Thorp, BH & Houston, B 1998, 'Cloning differentially regulated genes from chondrocytes using agarose gel differential display', Biochimica et Biophysica Acta - General Subjects, vol. 1396, no. 3, pp. 237-241. https://doi.org/10.1016/S0167-4781(97)00234-0

    Cloning differentially regulated genes from chondrocytes using agarose gel differential display. / Jefferies, David; Botman, M.; Farquharson, Colin; Lester, Douglas; Whitehead, C. C.; Thorp, B. H.; Houston, Brian.

    In: Biochimica et Biophysica Acta - General Subjects, Vol. 1396, No. 3, 13.03.1998, p. 237-241.

    Research output: Contribution to journalArticle

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    AU - Thorp, B. H.

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