Cloning differentially regulated genes from chondrocytes using agarose gel differential display

David Jefferies, M. Botman, Colin Farquharson, Douglas Lester, C. C. Whitehead, B. H. Thorp, Brian Houston

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)


    The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.

    Original languageEnglish
    Pages (from-to)237-241
    Number of pages5
    JournalBiochimica et Biophysica Acta - General Subjects
    Issue number3
    Publication statusPublished - 13 Mar 1998


    • Agarose gel differential display
    • Chondrocyte hypertrophy
    • Cell culture
    • Gene expression


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