Abstract
The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 237-241 |
| Number of pages | 5 |
| Journal | Biochimica et Biophysica Acta - General Subjects |
| Volume | 1396 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 13 Mar 1998 |
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Cloning differentially regulated genes from chondrocytes using agarose gel differential display. / Jefferies, David; Botman, M.; Farquharson, Colin; Lester, Douglas; Whitehead, C. C.; Thorp, B. H.; Houston, Brian.
In: Biochimica et Biophysica Acta - General Subjects, Vol. 1396, No. 3, 13.03.1998, p. 237-241.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cloning differentially regulated genes from chondrocytes using agarose gel differential display
AU - Jefferies, David
AU - Botman, M.
AU - Farquharson, Colin
AU - Lester, Douglas
AU - Whitehead, C. C.
AU - Thorp, B. H.
AU - Houston, Brian
PY - 1998/3/13
Y1 - 1998/3/13
N2 - The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.
AB - The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.
U2 - 10.1016/S0167-4781(97)00234-0
DO - 10.1016/S0167-4781(97)00234-0
M3 - Article
VL - 1396
SP - 237
EP - 241
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 3
ER -