Cloning differentially regulated genes from chondrocytes using agarose gel differential display

David Jefferies; M. Botman; Colin Farquharson; Douglas Lester; C. C. Whitehead; B. H. Thorp; Brian Houston

doi:10.1016/S0167-4781(97)00234-0

Cloning differentially regulated genes from chondrocytes using agarose gel differential display

David Jefferies, M. Botman, Colin Farquharson, Douglas Lester, C. C. Whitehead, B. H. Thorp, Brian Houston

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.

Original language	English
Pages (from-to)	237-241
Number of pages	5
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1396
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(97)00234-0
Publication status	Published - 13 Mar 1998

Access to Document

10.1016/S0167-4781(97)00234-0

Fingerprint Dive into the research topics of 'Cloning differentially regulated genes from chondrocytes using agarose gel differential display'. Together they form a unique fingerprint.

Cite this

@article{b920900cf92f402ab1ce1cc5edd3b975,

title = "Cloning differentially regulated genes from chondrocytes using agarose gel differential display",

abstract = "The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.",

author = "David Jefferies and M. Botman and Colin Farquharson and Douglas Lester and Whitehead, {C. C.} and Thorp, {B. H.} and Brian Houston",

year = "1998",

month = mar,

day = "13",

doi = "10.1016/S0167-4781(97)00234-0",

language = "English",

volume = "1396",

pages = "237--241",

journal = "Biochimica et Biophysica Acta - General Subjects",

issn = "0304-4165",

publisher = "Elsevier",

number = "3",

}

Cloning differentially regulated genes from chondrocytes using agarose gel differential display. / Jefferies, David; Botman, M.; Farquharson, Colin; Lester, Douglas; Whitehead, C. C.; Thorp, B. H.; Houston, Brian.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1396, No. 3, 13.03.1998, p. 237-241.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cloning differentially regulated genes from chondrocytes using agarose gel differential display

AU - Jefferies, David

AU - Botman, M.

AU - Farquharson, Colin

AU - Lester, Douglas

AU - Whitehead, C. C.

AU - Thorp, B. H.

AU - Houston, Brian

PY - 1998/3/13

Y1 - 1998/3/13

N2 - The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.

AB - The technique of RNA differential display has been used extensively to clone differentially expressed genes from a wide variety of cells and tissues. Recently, a simplified method of cloning differential display products, separated on agarose gels, was described. Here we report an adaption of this method, using total RNA, to clone differentially expressed genes. The approach is simple and rapid, and requires only small quantities of total RNA. Utilising this approach, we have cloned three differentially regulated genes from chondrocytes stimulated to hypertrophy in vitro, and confirmed their pattern of expression by Northern blotting. These gene fragments were sequenced and found to correspond to known genes, although only one has previously been isolated from chondrocytes.

U2 - 10.1016/S0167-4781(97)00234-0

DO - 10.1016/S0167-4781(97)00234-0

M3 - Article

C2 - 9545570

VL - 1396

SP - 237

EP - 241

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 3

ER -