Comparison of transesterified palm stearin/sunflower oil blends catalyzed by Pseudomonas and Mucor javanicus lipase

L. P. Lim*, H. M. Ghazali, O. M. Lai, B. S. Chu

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Palm stearin and sunflower oil mixtures in three different ratio of 3:7, 5:5 and 7:3 (w/w) were subjected to transesterification catalyzed by immobilized lipases from Pseudomonas and Mucor javanicus in a solvent-free system. The ability of these lipases to alter the physical properties of the fat blends was studied. Slip melting point (SMP), differential scanning calorimetry and X-ray diffraction analyses were used to evaluate the physical properties of the transesterified blends. The percentage of free fatty acids liberated was also determined. Results indicated that the Pseudomonas lipase was catalytically more active than Mucor javanicus lipase as shown by marked changes in the triacylglycerol profile and fatty acids released. Pseudomonas lipase-catalyzed mixtures also showed a bigger drop in SMP and successfully changed the polymorphic form from the dominant β crystal form in control blends to β′ crystal form in the transesterified mixtures.

Original languageEnglish
Pages (from-to)103-114
Number of pages12
JournalJournal of Food Lipids
Volume8
Issue number2
DOIs
Publication statusPublished - Jun 2001
Externally publishedYes

Fingerprint

stearin
Mucor
sunflower oil
Pseudomonas
Lipase
melting point
crystals
physical properties
transesterification
Freezing
Melting point
differential scanning calorimetry
X-ray diffraction
Physical properties
free fatty acids
triacylglycerols
Crystals
fatty acids
Transesterification
Differential Scanning Calorimetry

Cite this

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title = "Comparison of transesterified palm stearin/sunflower oil blends catalyzed by Pseudomonas and Mucor javanicus lipase",
abstract = "Palm stearin and sunflower oil mixtures in three different ratio of 3:7, 5:5 and 7:3 (w/w) were subjected to transesterification catalyzed by immobilized lipases from Pseudomonas and Mucor javanicus in a solvent-free system. The ability of these lipases to alter the physical properties of the fat blends was studied. Slip melting point (SMP), differential scanning calorimetry and X-ray diffraction analyses were used to evaluate the physical properties of the transesterified blends. The percentage of free fatty acids liberated was also determined. Results indicated that the Pseudomonas lipase was catalytically more active than Mucor javanicus lipase as shown by marked changes in the triacylglycerol profile and fatty acids released. Pseudomonas lipase-catalyzed mixtures also showed a bigger drop in SMP and successfully changed the polymorphic form from the dominant β crystal form in control blends to β′ crystal form in the transesterified mixtures.",
author = "Lim, {L. P.} and Ghazali, {H. M.} and Lai, {O. M.} and Chu, {B. S.}",
year = "2001",
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}

Comparison of transesterified palm stearin/sunflower oil blends catalyzed by Pseudomonas and Mucor javanicus lipase. / Lim, L. P.; Ghazali, H. M.; Lai, O. M.; Chu, B. S.

In: Journal of Food Lipids, Vol. 8, No. 2, 06.2001, p. 103-114.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of transesterified palm stearin/sunflower oil blends catalyzed by Pseudomonas and Mucor javanicus lipase

AU - Lim, L. P.

AU - Ghazali, H. M.

AU - Lai, O. M.

AU - Chu, B. S.

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AB - Palm stearin and sunflower oil mixtures in three different ratio of 3:7, 5:5 and 7:3 (w/w) were subjected to transesterification catalyzed by immobilized lipases from Pseudomonas and Mucor javanicus in a solvent-free system. The ability of these lipases to alter the physical properties of the fat blends was studied. Slip melting point (SMP), differential scanning calorimetry and X-ray diffraction analyses were used to evaluate the physical properties of the transesterified blends. The percentage of free fatty acids liberated was also determined. Results indicated that the Pseudomonas lipase was catalytically more active than Mucor javanicus lipase as shown by marked changes in the triacylglycerol profile and fatty acids released. Pseudomonas lipase-catalyzed mixtures also showed a bigger drop in SMP and successfully changed the polymorphic form from the dominant β crystal form in control blends to β′ crystal form in the transesterified mixtures.

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