Correlation of glutamine synthetase activity with cell magnesium concentration during cell division in yeast synchronized by induction

Graeme M. Walker, James C. Thomson, Colin Slaughter, John H. Duffus

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Synchronization of cell division in the fission yeast Schizosaccharomyces pombe and the budding yeast Kluyveromyces fragilis was achieved by induction using the DNA synthesis inhibitor 2'-deoxyadenosine and by a magnesium-exhaustion technique. The activity of glutamine synthetase in these synchronized cultures oscillated. Variations in the intracellular magnesium concentration were also observed, and peaks in magnesium concentration correlated with peaks in enzyme activity. We suggest that the enzyme from yeast is unstable and that its activity is regulated in vivo by changes in the intracellular concentration of magnesium.
    Original languageEnglish
    Pages (from-to)535-537
    Number of pages3
    JournalJournal of General Microbiology
    Volume120
    Issue number2
    DOIs
    Publication statusPublished - 1 Oct 1980

    Keywords

    • Glutamine synthetase
    • Cell division
    • Yeast

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