Detection of 8-hydroxy-2'-deoxyguanosine as a marker of oxidative damage in DNA and germplasm exposed to cryogenic treatments

Jason W. Johnston, Isobel Pimbley, Keith Harding, Erica E. Benson

Research output: Contribution to journalArticle

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Abstract

An HPLC method has been optimised to measure 8-hydroxy-2'-deoxyguanosine (8OHdG) in DNA and germplasm with the objective of using the adduct as a marker of cryostorage stability. The encapsulation-dehydration cryopreservation protocol was adapted as a model system for assessing the formation of 8OHdG from alginate-encapsulated DNA of calf thymus (CT) and currant species (Ribes) exposed to temperatures of -20 and -196ºC. The presence of H2O2 exacerbated the formation of 8OHdG in encapsulated CT and Ribes DNA. Production of the oxidized adduct was lower in the plant system. A reduction in residual water following osmotic dehydration and evaporative desiccation was associated with reduced adduct formation in encapsulated DNA. No significant differences in 8OHdG adduct formation were observed in plants regenerated from cryopreserved Ribes meristems derived from genotypes known to have differential tolerance to cryopreservation.
Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalCryoLetters
Volume31
Issue number1
StatePublished - Jan 2010
Externally publishedYes

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DNA
Ribes
cryopreservation
germplasm
calves
currants
alginates
encapsulation
meristems
DNA damage
genotype
temperature
water
methodology
cryogenics

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Johnston, Jason W.; Pimbley, Isobel; Harding, Keith; Benson, Erica E. / Detection of 8-hydroxy-2'-deoxyguanosine as a marker of oxidative damage in DNA and germplasm exposed to cryogenic treatments.

In: CryoLetters, Vol. 31, No. 1, 01.2010, p. 1-13.

Research output: Contribution to journalArticle

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Detection of 8-hydroxy-2'-deoxyguanosine as a marker of oxidative damage in DNA and germplasm exposed to cryogenic treatments. / Johnston, Jason W.; Pimbley, Isobel; Harding, Keith; Benson, Erica E.

In: CryoLetters, Vol. 31, No. 1, 01.2010, p. 1-13.

Research output: Contribution to journalArticle

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