Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery

M. P. Cude*, C. D. Gwenin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys12 can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.

Original languageEnglish
Title of host publicationNanotechnology (General) - 218th ECS Meeting
EditorsO. Leonte, Z. Aguilar, C. Bock, E. Traversa
Place of PublicationPennington, NJ
PublisherThe Electrochemical Society
Pages79-89
Number of pages11
ISBN (Electronic)9781566778961
ISBN (Print)9781607682486
DOIs
Publication statusPublished - 25 Apr 2011
Externally publishedYes
EventNanotechnology General Session - 218th ECS Meeting - Las Vegas, NV, United States
Duration: 10 Oct 201015 Oct 2010

Publication series

NameECS Transactions
Number38
Volume33
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Conference

ConferenceNanotechnology General Session - 218th ECS Meeting
Country/TerritoryUnited States
CityLas Vegas, NV
Period10/10/1015/10/10

Keywords

  • Amino acids
  • Bacteriology
  • Enzymes
  • Gold coatings
  • Iron oxides
  • Magnetic nanoparticles
  • Metal nanoparticles
  • Sols
  • Superparamagnetism
  • Tumors

Cite this