Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery

M. P. Cude; C. D. Gwenin

doi:10.1149/1.3583517

Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery

M. P. Cude^*, C. D. Gwenin

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Citations (Scopus)

Abstract

Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys₁₂ can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.

Original language	English
Title of host publication	Nanotechnology (General) - 218th ECS Meeting
Editors	O. Leonte, Z. Aguilar, C. Bock, E. Traversa
Place of Publication	Pennington, NJ
Publisher	The Electrochemical Society
Pages	79-89
Number of pages	11
ISBN (Electronic)	9781566778961
ISBN (Print)	9781607682486
DOIs	https://doi.org/10.1149/1.3583517
Publication status	Published - 25 Apr 2011
Externally published	Yes
Event	Nanotechnology General Session - 218th ECS Meeting - Las Vegas, NV, United States Duration: 10 Oct 2010 → 15 Oct 2010

Publication series

Name	ECS Transactions
Number	38
Volume	33
ISSN (Print)	1938-5862
ISSN (Electronic)	1938-6737

Conference

Conference	Nanotechnology General Session - 218th ECS Meeting
Country/Territory	United States
City	Las Vegas, NV
Period	10/10/10 → 15/10/10

Keywords

Amino acids
Bacteriology
Enzymes
Gold coatings
Iron oxides
Magnetic nanoparticles
Metal nanoparticles
Sols
Superparamagnetism
Tumors

Access to Document

10.1149/1.3583517

Cite this

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title = "Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery",

abstract = "Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys12 can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.",

author = "Cude, {M. P.} and Gwenin, {C. D.}",

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Cude, MP & Gwenin, CD 2011, Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery. in O Leonte, Z Aguilar, C Bock & E Traversa (eds), Nanotechnology (General) - 218th ECS Meeting. ECS Transactions, no. 38, vol. 33, The Electrochemical Society, Pennington, NJ, pp. 79-89, Nanotechnology General Session - 218th ECS Meeting, Las Vegas, NV, United States, 10/10/10. https://doi.org/10.1149/1.3583517

Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery. / Cude, M. P.; Gwenin, C. D.

Nanotechnology (General) - 218th ECS Meeting. ed. / O. Leonte; Z. Aguilar; C. Bock; E. Traversa. Pennington, NJ : The Electrochemical Society, 2011. p. 79-89 (ECS Transactions; Vol. 33, No. 38).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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N2 - Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys12 can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.

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