@inproceedings{89dcd6ba4f984a858d9dd963c16c15b2,
title = "Development of gold coated superparamagnetic iron oxide nanoparticles for nitroreductase delivery",
abstract = "Directed enzyme prodrug therapies (DEPT) currently suffer from inherent problems associated with localising and retaining enzymes at tumours. Superparamagnetic iron oxide nanoparticles (SPION) have been synthesised (≈10 nm) and surface functionalised with gold by direct reduction and by attachment of pre-synthesised Au seeds; these Au-SPION sols are stable in aqueous solution and are susceptible to magnetic manipulation. We have genetically modified the nitroreductase (NTR) yfkO from Bacillus Licheniformis to include a sequence of 12 cysteine residues, this modified NTR retains activity with CB1954 and has been immobilised onto gold via the 12 Au-S bonds. The yfkO-cys12 can therefore be immobilised with controlled orientation onto the Au-SPION to allow a magnetically directed enzyme prodrug therapy (MDEPT) to be developed; creating a novel strategy to target and localise prodrug activation to cancer tumour sites using magnetism.",
author = "Cude, {M. P.} and Gwenin, {C. D.}",
year = "2011",
month = apr,
day = "25",
doi = "10.1149/1.3583517",
language = "English",
isbn = "9781607682486",
series = "ECS Transactions",
publisher = "The Electrochemical Society",
number = "38",
pages = "79--89",
editor = "O. Leonte and Z. Aguilar and C. Bock and E. Traversa",
booktitle = "Nanotechnology (General) - 218th ECS Meeting",
address = "United States",
note = "Nanotechnology General Session - 218th ECS Meeting ; Conference date: 10-10-2010 Through 15-10-2010",
}