Development of solid-phase RPA on a lateral flow device for the detection of pathogens related to sepsis

Alice Jane Heeroma, Christopher Gwenin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
1 Downloads (Pure)

Abstract

Population extended life expectancy has significantly increased the risk of septic shock in an ageing population. Sepsis affects roughly 20 million people every year, resulting in over 11 million deaths. The need for faster more accurate diagnostics and better management is therefore paramount in the fight to prevent these avoidable deaths. Here we report the development of a POC device with the ability to identify a broad range of pathogens on a lateral flow platform. Namely Gram-positive and Gram-negative bacteria. The simple to use laboratory device has the potential to be automated, thus enabling an operator to carry out solid-phase lysis and room temperature RPA in situ, providing accurate results in hours rather than days. Results show there is a potential for a fully automated device in which concepts described in this paper can be integrated into a lateral flow device.

Original languageEnglish
Article number4182
Number of pages14
JournalSensors (Switzerland)
Volume20
Issue number15
Early online date28 Jul 2020
DOIs
Publication statusPublished - 28 Jul 2020
Externally publishedYes

Keywords

  • Sepsis
  • Lateral flow immunoassay
  • Solid phase RPA
  • Point-of-care
  • Bacterial infections

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