Differential extracellular enzyme production in colonies of Coriolus versicolor, Phlebia radiata and Phlebia rufa: effect of gaseous regime

Nia A. White, Lynne Boddy

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Abstract

The effects of different gaseous regimes on the growth rate and extracellular enzyme location in vitro of colonies of Phlebia radiata, Phlebia rufa and Coriolus versicolor are reported. The two Phlebia species showed similar growth, extracellular enzyme and pH responses to gaseous composition (N2, O2 and CO2), but the responses of C. versicolor differed. Whilst maximum extension rates were obtained for all species under atmospheric gaseous composition, maximum biomass production occurred at 5% (v/v) O2 with 20% (v/v) CO2 for the Phlebia species and at 5% O2 with 60% CO2 for C. versicolor. The Phlebia species had a coenocytic margin (5–6 mm width) under atmospheric conditions, which increased in width with increasing percentage of CO2. Laccase and peroxidase activity were present throughout the septate region, but not in the coenocytic zone. With C. versicolor laccase and peroxidase activities appeared throughout the colony, but were more intense in the peripheral region, under all gaseous regimes. A laser densitometer, normally used to visualize proteins on electrophoresis strips, was used to estimate profiles of biomass and laccase-α-naphthol activity within colonies. Surface pH changed little in colonies of C. versicolor but dropped by over 1 pH unit from the margin inwards with the Phlebia species. The significance of these results is discussed in terms of ecological strategy and developmental versatility.
Original languageEnglish
Pages (from-to)2589-2598
Number of pages10
JournalJournal of General Microbiology
Volume138
Issue number12
DOIs
Publication statusPublished - 1 Dec 1992

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Phlebia
Coriolus versicolor
laccase
enzymes
peroxidase
densitometers
naphthols
electrophoresis
lasers
biomass production
biomass

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title = "Differential extracellular enzyme production in colonies of Coriolus versicolor, Phlebia radiata and Phlebia rufa: effect of gaseous regime",
abstract = "The effects of different gaseous regimes on the growth rate and extracellular enzyme location in vitro of colonies of Phlebia radiata, Phlebia rufa and Coriolus versicolor are reported. The two Phlebia species showed similar growth, extracellular enzyme and pH responses to gaseous composition (N2, O2 and CO2), but the responses of C. versicolor differed. Whilst maximum extension rates were obtained for all species under atmospheric gaseous composition, maximum biomass production occurred at 5{\%} (v/v) O2 with 20{\%} (v/v) CO2 for the Phlebia species and at 5{\%} O2 with 60{\%} CO2 for C. versicolor. The Phlebia species had a coenocytic margin (5–6 mm width) under atmospheric conditions, which increased in width with increasing percentage of CO2. Laccase and peroxidase activity were present throughout the septate region, but not in the coenocytic zone. With C. versicolor laccase and peroxidase activities appeared throughout the colony, but were more intense in the peripheral region, under all gaseous regimes. A laser densitometer, normally used to visualize proteins on electrophoresis strips, was used to estimate profiles of biomass and laccase-α-naphthol activity within colonies. Surface pH changed little in colonies of C. versicolor but dropped by over 1 pH unit from the margin inwards with the Phlebia species. The significance of these results is discussed in terms of ecological strategy and developmental versatility.",
author = "White, {Nia A.} and Lynne Boddy",
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N2 - The effects of different gaseous regimes on the growth rate and extracellular enzyme location in vitro of colonies of Phlebia radiata, Phlebia rufa and Coriolus versicolor are reported. The two Phlebia species showed similar growth, extracellular enzyme and pH responses to gaseous composition (N2, O2 and CO2), but the responses of C. versicolor differed. Whilst maximum extension rates were obtained for all species under atmospheric gaseous composition, maximum biomass production occurred at 5% (v/v) O2 with 20% (v/v) CO2 for the Phlebia species and at 5% O2 with 60% CO2 for C. versicolor. The Phlebia species had a coenocytic margin (5–6 mm width) under atmospheric conditions, which increased in width with increasing percentage of CO2. Laccase and peroxidase activity were present throughout the septate region, but not in the coenocytic zone. With C. versicolor laccase and peroxidase activities appeared throughout the colony, but were more intense in the peripheral region, under all gaseous regimes. A laser densitometer, normally used to visualize proteins on electrophoresis strips, was used to estimate profiles of biomass and laccase-α-naphthol activity within colonies. Surface pH changed little in colonies of C. versicolor but dropped by over 1 pH unit from the margin inwards with the Phlebia species. The significance of these results is discussed in terms of ecological strategy and developmental versatility.

AB - The effects of different gaseous regimes on the growth rate and extracellular enzyme location in vitro of colonies of Phlebia radiata, Phlebia rufa and Coriolus versicolor are reported. The two Phlebia species showed similar growth, extracellular enzyme and pH responses to gaseous composition (N2, O2 and CO2), but the responses of C. versicolor differed. Whilst maximum extension rates were obtained for all species under atmospheric gaseous composition, maximum biomass production occurred at 5% (v/v) O2 with 20% (v/v) CO2 for the Phlebia species and at 5% O2 with 60% CO2 for C. versicolor. The Phlebia species had a coenocytic margin (5–6 mm width) under atmospheric conditions, which increased in width with increasing percentage of CO2. Laccase and peroxidase activity were present throughout the septate region, but not in the coenocytic zone. With C. versicolor laccase and peroxidase activities appeared throughout the colony, but were more intense in the peripheral region, under all gaseous regimes. A laser densitometer, normally used to visualize proteins on electrophoresis strips, was used to estimate profiles of biomass and laccase-α-naphthol activity within colonies. Surface pH changed little in colonies of C. versicolor but dropped by over 1 pH unit from the margin inwards with the Phlebia species. The significance of these results is discussed in terms of ecological strategy and developmental versatility.

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