Differential phosphoinositide binding to components of the G protein-gated K+ channel

Alison M. Thomas, Sean G. Brown, Joanne L. Leaney, Andrew Tinker

Research output: Contribution to journalArticle

  • 11 Citations

Abstract

The regulation of ion channels and transporters by anionic phospholipids is currently very topical. G protein-gated K+ channels from the Kir3.0 family are involved in slowing the heart rate, generating late inhibitory postsynaptic potentials and controlling hormone release from neuroendocrine cells. There is considerable functional precedent for the control of these channels by phosphatidylinositol 4,5-bisphosphate. In this study, we used a biochemical assay to investigate the lipid binding properties of Kir3.0 channel domains. We reveal a differential binding affinity to a range of phosphoinositides between the C termini of the Kir3.0 isoforms. Furthermore, the N terminus in addition to the C terminus of Kir3.4 is necessary to observe binding and is decreased by the mutations R72A, K195A and R196A but not K194A. Protein kinase C phosphorylation of the Kir3.1 C-terminal fusion protein decreases anionic phospholipid binding. The differential binding affinity has functional consequences as the inhibition of homomeric Kir3.1, occurring after M3 receptor activation, recovers over minutes while homomeric Kir3.2 does not.
Original languageEnglish
Pages (from-to)43-53
Number of pages11
JournalJournal of Membrane Biology
Volume211
Issue number1
DOIs
StatePublished - May 2006

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Phosphatidylinositols
GTP-Binding Proteins
Phospholipids
Neuroendocrine Cells
Inhibitory Postsynaptic Potentials
Ion Channels
Protein Kinase C
Protein Isoforms
Heart Rate
Phosphorylation
Hormones
Lipids
Mutation
Proteins

Cite this

Thomas, Alison M.; Brown, Sean G.; Leaney, Joanne L.; Tinker, Andrew / Differential phosphoinositide binding to components of the G protein-gated K+ channel.

In: Journal of Membrane Biology, Vol. 211, No. 1, 05.2006, p. 43-53.

Research output: Contribution to journalArticle

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Differential phosphoinositide binding to components of the G protein-gated K+ channel. / Thomas, Alison M.; Brown, Sean G.; Leaney, Joanne L.; Tinker, Andrew.

In: Journal of Membrane Biology, Vol. 211, No. 1, 05.2006, p. 43-53.

Research output: Contribution to journalArticle

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AU - Leaney,Joanne L.

AU - Tinker,Andrew

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AB - The regulation of ion channels and transporters by anionic phospholipids is currently very topical. G protein-gated K+ channels from the Kir3.0 family are involved in slowing the heart rate, generating late inhibitory postsynaptic potentials and controlling hormone release from neuroendocrine cells. There is considerable functional precedent for the control of these channels by phosphatidylinositol 4,5-bisphosphate. In this study, we used a biochemical assay to investigate the lipid binding properties of Kir3.0 channel domains. We reveal a differential binding affinity to a range of phosphoinositides between the C termini of the Kir3.0 isoforms. Furthermore, the N terminus in addition to the C terminus of Kir3.4 is necessary to observe binding and is decreased by the mutations R72A, K195A and R196A but not K194A. Protein kinase C phosphorylation of the Kir3.1 C-terminal fusion protein decreases anionic phospholipid binding. The differential binding affinity has functional consequences as the inhibition of homomeric Kir3.1, occurring after M3 receptor activation, recovers over minutes while homomeric Kir3.2 does not.

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