Effect of liquid nitrogen pre-treatment on various types of wool waste fibres for biogas production

Elena Kuzmanova, Nikolai Zhelev, Joseph C. Akunna*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)
    107 Downloads (Pure)


    This study investigated the role of liquid nitrogen (LN2) in increasing microbial accessibility of wool proteins for biogas production. It involves a mechanical size reduction of four different types of raw wool fibres, namely, Blackface, Bluefaced Leicester, Texel and Scotch Mule, in presence of liquid nitrogen, followed by the determination of the methane production potential of the pre-treated wool fibres. The highest methane yield, 157.3 cm3 g−1 VS, was obtained from pre-treated Scotch mule wool fibre culture, and represented more than 80% increase when compared to the yield obtained from its raw equivalent culture. The increase in biogas yield was attributed to the effectiveness of LN2 in enhancing particle size reduction and the consequent increase in wool solubility and bioavailability. Results also showed that LN2 pre-treatment can enhance size reduction but has limited effect on the molecular structure. The study also showed that the biogas potential of waste wool fibres varies with the type and source of wool.
    Original languageEnglish
    Article numbere00619
    Number of pages11
    Issue number5
    Early online date2 May 2018
    Publication statusPublished - May 2018


    • Environmental science
    • Energy
    • Materials science


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