Effect of salinity on the synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase in barley leaves

T. S. Miteva, N. Zh. Zhelev, L. P. Popova*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) (RubisCO) was quantified in 7-day-old leaves of barley (Hordeum vulgare L., var. Alfa) treated with NaCl using polyacrylamide gel electrophoresis. The treated plants showed a decrease in the level of RubisCO. In the presence of 100 mM NaCl the level of total soluble protein on a fresh weight basis was about 64% of the control, whereas the level of RubisCO was only about 20% that of control plants.

Salinity induced marked quantitative and qualitative changes in polypeptide profiles of soluble leaf proteins. Enhanced levels of 76, 60, 47, 43 and 30 kD polypeptides and reduced levels of 55 and 15 kD polypeptides were observed in NaCl-treated plants. The appearance of one new polypeptide of 26-25 kD was found in NaCl-treated samples.

The influence of salinity on the synthesis of RubisCO was followed by in vivo labeling with a 14C-amino acid mixture (14C-AAM). The results demonstrate that salinity inhibits the synthesis of total soluble protein with a more pronounced inhibition of synthesis of RubisCO.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalJournal of Plant Physiology
Volume140
Issue number1
DOIs
Publication statusPublished - May 1992
Externally publishedYes

Fingerprint

Ribulose-Bisphosphate Carboxylase
Oxygenases
Salinity
ribulose 1,5-diphosphate
ribulose-bisphosphate carboxylase
Hordeum
oxygenases
barley
salinity
synthesis
polypeptides
Peptides
leaves
Proteins
leaf protein
Polyacrylamide Gel Electrophoresis
Hordeum vulgare
polyacrylamide gel electrophoresis
ribulose-1,5 diphosphate
Amino Acids

Cite this

Miteva, T. S. ; Zh. Zhelev, N. ; Popova, L. P. / Effect of salinity on the synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase in barley leaves. In: Journal of Plant Physiology. 1992 ; Vol. 140, No. 1. pp. 46-51.
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abstract = "Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) (RubisCO) was quantified in 7-day-old leaves of barley (Hordeum vulgare L., var. Alfa) treated with NaCl using polyacrylamide gel electrophoresis. The treated plants showed a decrease in the level of RubisCO. In the presence of 100 mM NaCl the level of total soluble protein on a fresh weight basis was about 64{\%} of the control, whereas the level of RubisCO was only about 20{\%} that of control plants.Salinity induced marked quantitative and qualitative changes in polypeptide profiles of soluble leaf proteins. Enhanced levels of 76, 60, 47, 43 and 30 kD polypeptides and reduced levels of 55 and 15 kD polypeptides were observed in NaCl-treated plants. The appearance of one new polypeptide of 26-25 kD was found in NaCl-treated samples.The influence of salinity on the synthesis of RubisCO was followed by in vivo labeling with a 14C-amino acid mixture (14C-AAM). The results demonstrate that salinity inhibits the synthesis of total soluble protein with a more pronounced inhibition of synthesis of RubisCO.",
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Effect of salinity on the synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase in barley leaves. / Miteva, T. S.; Zh. Zhelev, N.; Popova, L. P.

In: Journal of Plant Physiology, Vol. 140, No. 1, 05.1992, p. 46-51.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of salinity on the synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase in barley leaves

AU - Miteva, T. S.

AU - Zh. Zhelev, N.

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AB - Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) (RubisCO) was quantified in 7-day-old leaves of barley (Hordeum vulgare L., var. Alfa) treated with NaCl using polyacrylamide gel electrophoresis. The treated plants showed a decrease in the level of RubisCO. In the presence of 100 mM NaCl the level of total soluble protein on a fresh weight basis was about 64% of the control, whereas the level of RubisCO was only about 20% that of control plants.Salinity induced marked quantitative and qualitative changes in polypeptide profiles of soluble leaf proteins. Enhanced levels of 76, 60, 47, 43 and 30 kD polypeptides and reduced levels of 55 and 15 kD polypeptides were observed in NaCl-treated plants. The appearance of one new polypeptide of 26-25 kD was found in NaCl-treated samples.The influence of salinity on the synthesis of RubisCO was followed by in vivo labeling with a 14C-amino acid mixture (14C-AAM). The results demonstrate that salinity inhibits the synthesis of total soluble protein with a more pronounced inhibition of synthesis of RubisCO.

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