Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells

Linda K. Adams, Erica E. Benson*, Harry J. Staines, David H. Bremner, Stephen Millam, Nigel Deighton

*Corresponding author for this work

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.

    Original languageEnglish
    Pages (from-to)376-386
    Number of pages11
    JournalJournal of Plant Physiology
    Volume155
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 1999

    Fingerprint

    Plant Cells
    Malondialdehyde
    malondialdehyde
    Lipid Peroxidation
    lipid peroxidation
    Daucus carota
    Toxicology
    cells
    Embryonic Structures
    Plant Development
    Bony Callus
    callus culture
    Biotechnology
    somatic embryos
    Aldehydes
    embryo (plant)
    Mental Competency
    biotechnology
    aldehydes
    Embryonic Development

    Cite this

    Adams, Linda K. ; Benson, Erica E. ; Staines, Harry J. ; Bremner, David H. ; Millam, Stephen ; Deighton, Nigel. / Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells. In: Journal of Plant Physiology. 1999 ; Vol. 155, No. 3. pp. 376-386.
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    abstract = "The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.",
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    Effects of the lipid peroxidation products 4-hydroxy-2-nonenal and malondialdehyde on the proliferation and morphogenetic development of in vitro plant cells. / Adams, Linda K.; Benson, Erica E.; Staines, Harry J.; Bremner, David H.; Millam, Stephen; Deighton, Nigel.

    In: Journal of Plant Physiology, Vol. 155, No. 3, 01.01.1999, p. 376-386.

    Research output: Contribution to journalArticle

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    AU - Adams, Linda K.

    AU - Benson, Erica E.

    AU - Staines, Harry J.

    AU - Bremner, David H.

    AU - Millam, Stephen

    AU - Deighton, Nigel

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    AB - The purpose of this study was to evaluate the toxicological effects of 4-hydroxy-2-nonenal (HNE) and malondialdehyde (MDA) on in vitro plant cells using the model morphogenetic system of Daucus carota L. for the production of somatic embryos. It was found that both HNE and MDA inhibited proliferative growth and embryogenesis in D. carota cultures. However, embryogenic capacity was restored when callus cultures were transferred to embryo induction medium without HNE or MDA and the recovery of morphogenetic competence was more pronounced in cultures which had been exposed to MDA as compared to those which had been treated with HNE. Also, differential responses between embryogenic and nonembryogenic cultures were observed in relation to the delivery of aldehydes in different culture media. This paper reports, for the first time, the toxicological effects of HNE and MDA on in vitro plant cells. The significance of these findings in relation to the control of in vitro development in plant tissue cultures which are used in biotechnology programmes is discussed.

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