Elasticity of human embryonic stem cells as determined by atomic force microscopy

Robert Kiss, Henry Bock, Steve Pells, Elisabetta Canetta, Ashok K. Adya, Andrew J. Moore, Paul De Sousa, Nicholas A. Willoughby

Research output: Contribution to journalArticle

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Abstract

The expansive growth and differentiation potential of human embryonic stem cells (hESCs) make them a promising source of cells for regenerative medicine. However, this promise is off set by the propensity for spontaneous or uncontrolled differentiation to result in heterogeneous cell populations. Cell elasticity has recently been shown to characterize particular cell phenotypes, with undifferentiated and differentiated cells sometimes showing significant differences in their elasticities. In this study, we determined the Young's modulus of hESCs by atomic force microscopy using a pyramidal tip. Using this method we are able to take point measurements of elasticity at multiple locations on a single cell, allowing local variations due to cell structure to be identified. We found considerable differences in the elasticity of the analyzed hESCs, reflected by a broad range of Young's modulus (0.05-10 kPa). This surprisingly high variation suggests that elasticity could serve as the basis of a simple and efficient large scale purification/separation technique to discriminate subpopulations of hESCs.
Original languageEnglish
Article number101009
Number of pages9
JournalJournal of Biomechanical Engineering
Volume133
Issue number10
DOIs
StatePublished - Oct 2011

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Atomic Force Microscopy
Elasticity
Embryonic Stem Cells
Stem cells
Atomic force microscopy
Elastic moduli
Elastic Modulus
Purification
Cells
Regenerative Medicine
Phenotype

Cite this

Kiss, R., Bock, H., Pells, S., Canetta, E., Adya, A. K., Moore, A. J., ... Willoughby, N. A. (2011). Elasticity of human embryonic stem cells as determined by atomic force microscopy. Journal of Biomechanical Engineering, 133(10), [101009]. DOI: 10.1115/1.4005286

Kiss, Robert; Bock, Henry; Pells, Steve; Canetta, Elisabetta; Adya, Ashok K.; Moore, Andrew J.; De Sousa, Paul; Willoughby, Nicholas A. / Elasticity of human embryonic stem cells as determined by atomic force microscopy.

In: Journal of Biomechanical Engineering, Vol. 133, No. 10, 101009, 10.2011.

Research output: Contribution to journalArticle

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abstract = "The expansive growth and differentiation potential of human embryonic stem cells (hESCs) make them a promising source of cells for regenerative medicine. However, this promise is off set by the propensity for spontaneous or uncontrolled differentiation to result in heterogeneous cell populations. Cell elasticity has recently been shown to characterize particular cell phenotypes, with undifferentiated and differentiated cells sometimes showing significant differences in their elasticities. In this study, we determined the Young's modulus of hESCs by atomic force microscopy using a pyramidal tip. Using this method we are able to take point measurements of elasticity at multiple locations on a single cell, allowing local variations due to cell structure to be identified. We found considerable differences in the elasticity of the analyzed hESCs, reflected by a broad range of Young's modulus (0.05-10 kPa). This surprisingly high variation suggests that elasticity could serve as the basis of a simple and efficient large scale purification/separation technique to discriminate subpopulations of hESCs.",
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Kiss, R, Bock, H, Pells, S, Canetta, E, Adya, AK, Moore, AJ, De Sousa, P & Willoughby, NA 2011, 'Elasticity of human embryonic stem cells as determined by atomic force microscopy' Journal of Biomechanical Engineering, vol 133, no. 10, 101009. DOI: 10.1115/1.4005286

Elasticity of human embryonic stem cells as determined by atomic force microscopy. / Kiss, Robert; Bock, Henry; Pells, Steve; Canetta, Elisabetta; Adya, Ashok K.; Moore, Andrew J.; De Sousa, Paul; Willoughby, Nicholas A.

In: Journal of Biomechanical Engineering, Vol. 133, No. 10, 101009, 10.2011.

Research output: Contribution to journalArticle

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AB - The expansive growth and differentiation potential of human embryonic stem cells (hESCs) make them a promising source of cells for regenerative medicine. However, this promise is off set by the propensity for spontaneous or uncontrolled differentiation to result in heterogeneous cell populations. Cell elasticity has recently been shown to characterize particular cell phenotypes, with undifferentiated and differentiated cells sometimes showing significant differences in their elasticities. In this study, we determined the Young's modulus of hESCs by atomic force microscopy using a pyramidal tip. Using this method we are able to take point measurements of elasticity at multiple locations on a single cell, allowing local variations due to cell structure to be identified. We found considerable differences in the elasticity of the analyzed hESCs, reflected by a broad range of Young's modulus (0.05-10 kPa). This surprisingly high variation suggests that elasticity could serve as the basis of a simple and efficient large scale purification/separation technique to discriminate subpopulations of hESCs.

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Kiss R, Bock H, Pells S, Canetta E, Adya AK, Moore AJ et al. Elasticity of human embryonic stem cells as determined by atomic force microscopy. Journal of Biomechanical Engineering. 2011 Oct;133(10). 101009. Available from, DOI: 10.1115/1.4005286