Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382

Tony Gutierrez, Vincent V. Leo, Graeme M. Walker, David H. Green

Research output: Contribution to journalArticle

  • 15 Citations

Abstract

We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382, was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis and lyophilization, a chemical analysis of the resultant dried polymer revealed it to be a glycoprotein composed of 10.9% protein, 23.3% carbohydrate and a 5.5% uronic acid content. At relatively low concentrations (0.02%, w/v), E-382 was found to form oil-in-water emulsions against hydrocarbon and food oils under neutral pH and acidic conditions. The most stable emulsions were formed against the oils sunflower, vegetable and ground nut under neutral pH conditions. Aqueous solutions of the polymer were viscous, and its reduced viscosity (ηred) was determined to be 0.54 m3/kg. Although proteins and uronic acids may possess surface-active properties, the viscosifying effect of this polymer, which is a typical feature of some commercial hydrocolloids, is more likely to confer its high emulsion-stabilizing qualities.
Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalEnzyme and Microbial Technology
Volume45
Issue number1
DOIs
StatePublished - Jul 2009

Fingerprint

Glycoproteins
Polymers
Emulsions
Uronic Acids
Proteins
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Mineral Oil
Nuts
Plant Oils
Freeze Drying
Surface Properties
Helianthus
Colloids
Viscosity
Dialysis
Oils
Ethanol
Carbohydrates
Glucose
Food

Cite this

Gutierrez, Tony; Leo, Vincent V.; Walker, Graeme M.; Green, David H. / Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382.

In: Enzyme and Microbial Technology, Vol. 45, No. 1, 07.2009, p. 53-57.

Research output: Contribution to journalArticle

@article{c200c72a39c949eab4a7089283bc6f41,
title = "Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382",
abstract = "We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382, was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis and lyophilization, a chemical analysis of the resultant dried polymer revealed it to be a glycoprotein composed of 10.9% protein, 23.3% carbohydrate and a 5.5% uronic acid content. At relatively low concentrations (0.02%, w/v), E-382 was found to form oil-in-water emulsions against hydrocarbon and food oils under neutral pH and acidic conditions. The most stable emulsions were formed against the oils sunflower, vegetable and ground nut under neutral pH conditions. Aqueous solutions of the polymer were viscous, and its reduced viscosity (ηred) was determined to be 0.54 m3/kg. Although proteins and uronic acids may possess surface-active properties, the viscosifying effect of this polymer, which is a typical feature of some commercial hydrocolloids, is more likely to confer its high emulsion-stabilizing qualities.",
author = "Tony Gutierrez and Leo, {Vincent V.} and Walker, {Graeme M.} and Green, {David H.}",
year = "2009",
month = "7",
doi = "10.1016/j.enzmictec.2009.04.001",
volume = "45",
pages = "53--57",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier Inc.",
number = "1",

}

Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382. / Gutierrez, Tony; Leo, Vincent V.; Walker, Graeme M.; Green, David H.

In: Enzyme and Microbial Technology, Vol. 45, No. 1, 07.2009, p. 53-57.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Emulsifying properties of a glycoprotein extract produced by a marine Flexibacter species strain TG382

AU - Gutierrez,Tony

AU - Leo,Vincent V.

AU - Walker,Graeme M.

AU - Green,David H.

PY - 2009/7

Y1 - 2009/7

N2 - We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382, was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis and lyophilization, a chemical analysis of the resultant dried polymer revealed it to be a glycoprotein composed of 10.9% protein, 23.3% carbohydrate and a 5.5% uronic acid content. At relatively low concentrations (0.02%, w/v), E-382 was found to form oil-in-water emulsions against hydrocarbon and food oils under neutral pH and acidic conditions. The most stable emulsions were formed against the oils sunflower, vegetable and ground nut under neutral pH conditions. Aqueous solutions of the polymer were viscous, and its reduced viscosity (ηred) was determined to be 0.54 m3/kg. Although proteins and uronic acids may possess surface-active properties, the viscosifying effect of this polymer, which is a typical feature of some commercial hydrocolloids, is more likely to confer its high emulsion-stabilizing qualities.

AB - We report, for the first time, on the production of an emulsifying polymer produced by a Flexibacter species (designated strain TG382). This polymer, E-382, was produced extracellularly during growth of the organism in a marine broth amended with glucose. After cold ethanol precipitation, extensive dialysis and lyophilization, a chemical analysis of the resultant dried polymer revealed it to be a glycoprotein composed of 10.9% protein, 23.3% carbohydrate and a 5.5% uronic acid content. At relatively low concentrations (0.02%, w/v), E-382 was found to form oil-in-water emulsions against hydrocarbon and food oils under neutral pH and acidic conditions. The most stable emulsions were formed against the oils sunflower, vegetable and ground nut under neutral pH conditions. Aqueous solutions of the polymer were viscous, and its reduced viscosity (ηred) was determined to be 0.54 m3/kg. Although proteins and uronic acids may possess surface-active properties, the viscosifying effect of this polymer, which is a typical feature of some commercial hydrocolloids, is more likely to confer its high emulsion-stabilizing qualities.

U2 - 10.1016/j.enzmictec.2009.04.001

DO - 10.1016/j.enzmictec.2009.04.001

M3 - Article

VL - 45

SP - 53

EP - 57

JO - Enzyme and Microbial Technology

T2 - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 1

ER -