Exercise redox biochemistry

conceptual, methodological and technical recommendations

James N. Cobley, Graeme L. Close, Damian M. Bailey, Gareth W. Davison

Research output: Contribution to journalArticle

20 Citations (Scopus)
28 Downloads (Pure)

Abstract

Exercise redox biochemistry is of considerable interest owing to its translational value in health and disease. However, unaddressed conceptual, methodological and technical issues complicate attempts to unravel how exercise alters redox homeostasis in health and disease. Conceptual issues relate to misunderstandings that arise when the chemical heterogeneity of redox biology is disregarded which often complicate attempts to use redox-active compounds and assess redox signalling. Further, that oxidised macromolecule adduct levels reflect formation and repair is seldom considered. Methodological and technical issues relate to the use of out-dated assays and/or inappropriate sample preparation techniques that confound biochemical redox analysis. After considering each of the aforementioned issues, we outline how each issue can be resolved and provide a unifying set of recommendations. We specifically recommend that investigators: consider chemical heterogeneity, use redox-active compounds judiciously, abandon flawed assays, carefully prepare samples and assay buffers, consider repair/metabolism, use multiple biomarkers to assess oxidative damage and redox signalling.
Original languageEnglish
Pages (from-to)540-548
Number of pages9
JournalRedox Biology
Volume12
Early online date27 Mar 2017
DOIs
Publication statusPublished - Aug 2017

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Biochemistry
Oxidation-Reduction
Assays
Repair
Health
Biomarkers
Macromolecules
Metabolism
Buffers
Homeostasis
Research Personnel

Cite this

Cobley, James N. ; Close, Graeme L. ; Bailey, Damian M. ; Davison, Gareth W. / Exercise redox biochemistry : conceptual, methodological and technical recommendations. In: Redox Biology. 2017 ; Vol. 12. pp. 540-548.
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Exercise redox biochemistry : conceptual, methodological and technical recommendations. / Cobley, James N.; Close, Graeme L.; Bailey, Damian M.; Davison, Gareth W.

In: Redox Biology, Vol. 12, 08.2017, p. 540-548.

Research output: Contribution to journalArticle

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