Expression patterns of chondrocyte genes cloned by differential display in tibial dyschondroplasia

David Jefferies, Brian Houston, Douglas H. Lester, C. C. Whitehead, B. H. Thorp, M. Botman, Colin Farquharson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Tibial dyschondroplasia (TD) appears to involve a failure of the growth plate chondrocytes within growing long bones to differentiate fully to the hypertrophic stage, resulting in a mass of prehypertrophic chondrocytes which form the avascular TD lesion. Many biochemical and molecular markers of chondrocyte hypertrophy are absent from the lesion, or show reduced expression, but the cause of the disorder remains to be identified. As differentiation to the hypertrophic state is impaired in TD, we hypothesised that chondrocyte genes that are differentially expressed in the growth plate should show altered expression in TD. Using differential display, four genes, B-cadherin, EF2, HT7 and Ex-FABP were cloned from chondrocytes stimulated to differentiate to the hypertrophic stage in vitro, and their differential expression confirmed in vivo. Using semi-quantitative RT-PCR, the expression patterns of these genes were compared in chondrocytes from normal and TD growth plates. Surprisingly, none of these genes showed the pattern of expression that might be expected in TD lesion chondrocytes, and two of them, B-cadherin and Ex-FABP, were upregulated in the lesion. This indicates that the TD phenotype does not merely reflect the absence of hypertrophic marker genes, but may be influenced by more complex developmental mechanisms/defects than previously thought.
Original languageEnglish
Pages (from-to)180-188
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1501
Issue number2-3
Early online date31 May 2000
DOIs
Publication statusPublished - 15 Jun 2000

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Osteochondrodysplasias
Chondrocytes
Growth Plate
Genes
Cadherins
Hypertrophy
Biomarkers
Phenotype
Gene Expression
Bone and Bones
Polymerase Chain Reaction

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Jefferies, D., Houston, B., Lester, D. H., Whitehead, C. C., Thorp, B. H., Botman, M., & Farquharson, C. (2000). Expression patterns of chondrocyte genes cloned by differential display in tibial dyschondroplasia. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1501(2-3), 180-188. https://doi.org/10.1016/S0925-4439(00)00020-X
Jefferies, David ; Houston, Brian ; Lester, Douglas H. ; Whitehead, C. C. ; Thorp, B. H. ; Botman, M. ; Farquharson, Colin. / Expression patterns of chondrocyte genes cloned by differential display in tibial dyschondroplasia. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2000 ; Vol. 1501, No. 2-3. pp. 180-188.
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Jefferies, D, Houston, B, Lester, DH, Whitehead, CC, Thorp, BH, Botman, M & Farquharson, C 2000, 'Expression patterns of chondrocyte genes cloned by differential display in tibial dyschondroplasia', Biochimica et Biophysica Acta - Molecular Basis of Disease, vol. 1501, no. 2-3, pp. 180-188. https://doi.org/10.1016/S0925-4439(00)00020-X

Expression patterns of chondrocyte genes cloned by differential display in tibial dyschondroplasia. / Jefferies, David; Houston, Brian; Lester, Douglas H.; Whitehead, C. C.; Thorp, B. H.; Botman, M.; Farquharson, Colin.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1501, No. 2-3, 15.06.2000, p. 180-188.

Research output: Contribution to journalArticle

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