Factors affecting droplet size of sodium caseinate-stabilized O/W emulsions containing β-carotene

Sumiyo Kanafusa, Boon-Seang Chu, Mitsutoshi Nakajima*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This work was initiated to prepare an oil-in-water (O/W) emulsion containing β-carotene by microfluidization. The β-carotene was dissolved in triolein and microfluidized with an aqueous phase containing sodium caseinate (SC) as the emulsifier. Microfluization at 140 MPa resulted in O/W emulsions with a mean droplet diameter of ca. 120 nm, which was further confirmed by transmission electron microscopy analysis. The influences of SC concentration and microfluidization parameters on the droplet size of the emulsions were studied. The results showed that the mean droplet diameter decreased significantly (p <0.05) from 310 to 93 nm with the increase in SC concentration from 0.1 to 2 wt-%. However, a further increase in SC concentration did not much change the droplet diameter, although the polydispersity of the emulsions was slightly improved. The droplet diameter of the emulsions was found to decrease from 200 to 120 nm with increasing microfluidization pressure, with narrower droplet size distribution. The storage study showed that the emulsions were physically stable for about 2 weeks at 4°C in the dark. The results provide a better understanding of the performance of SC in stabilizing the O/W emulsions.

Original languageEnglish
Pages (from-to)1038-1041
Number of pages4
JournalEuropean Journal of Lipid Science and Technology
Volume109
Issue number10
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

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sodium caseinate
droplet size
Carotenoids
carotenes
Caseins
Emulsions
emulsions
Oils
Sodium
Water
droplets
Triolein
triolein
emulsifiers
Polydispersity
Transmission Electron Microscopy
transmission electron microscopy
Transmission electron microscopy
Pressure

Cite this

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title = "Factors affecting droplet size of sodium caseinate-stabilized O/W emulsions containing β-carotene",
abstract = "This work was initiated to prepare an oil-in-water (O/W) emulsion containing β-carotene by microfluidization. The β-carotene was dissolved in triolein and microfluidized with an aqueous phase containing sodium caseinate (SC) as the emulsifier. Microfluization at 140 MPa resulted in O/W emulsions with a mean droplet diameter of ca. 120 nm, which was further confirmed by transmission electron microscopy analysis. The influences of SC concentration and microfluidization parameters on the droplet size of the emulsions were studied. The results showed that the mean droplet diameter decreased significantly (p <0.05) from 310 to 93 nm with the increase in SC concentration from 0.1 to 2 wt-{\%}. However, a further increase in SC concentration did not much change the droplet diameter, although the polydispersity of the emulsions was slightly improved. The droplet diameter of the emulsions was found to decrease from 200 to 120 nm with increasing microfluidization pressure, with narrower droplet size distribution. The storage study showed that the emulsions were physically stable for about 2 weeks at 4°C in the dark. The results provide a better understanding of the performance of SC in stabilizing the O/W emulsions.",
author = "Sumiyo Kanafusa and Boon-Seang Chu and Mitsutoshi Nakajima",
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Factors affecting droplet size of sodium caseinate-stabilized O/W emulsions containing β-carotene. / Kanafusa, Sumiyo; Chu, Boon-Seang; Nakajima, Mitsutoshi.

In: European Journal of Lipid Science and Technology, Vol. 109, No. 10, 10.2007, p. 1038-1041.

Research output: Contribution to journalArticle

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AU - Chu, Boon-Seang

AU - Nakajima, Mitsutoshi

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AB - This work was initiated to prepare an oil-in-water (O/W) emulsion containing β-carotene by microfluidization. The β-carotene was dissolved in triolein and microfluidized with an aqueous phase containing sodium caseinate (SC) as the emulsifier. Microfluization at 140 MPa resulted in O/W emulsions with a mean droplet diameter of ca. 120 nm, which was further confirmed by transmission electron microscopy analysis. The influences of SC concentration and microfluidization parameters on the droplet size of the emulsions were studied. The results showed that the mean droplet diameter decreased significantly (p <0.05) from 310 to 93 nm with the increase in SC concentration from 0.1 to 2 wt-%. However, a further increase in SC concentration did not much change the droplet diameter, although the polydispersity of the emulsions was slightly improved. The droplet diameter of the emulsions was found to decrease from 200 to 120 nm with increasing microfluidization pressure, with narrower droplet size distribution. The storage study showed that the emulsions were physically stable for about 2 weeks at 4°C in the dark. The results provide a better understanding of the performance of SC in stabilizing the O/W emulsions.

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