Abstract
The theoretical possibility of applying gene transfer methodologies to the human germline is explored. Transgenic methods for genetically manipulating embryos may in principle be applied to humans. In particular, microinjection of retroviral vector appears to hold the greatest promise, with transgenic primates already obtained from this approach. Sperm-mediated gene transfer offers potentially the easiest route to the human germline, however the requisite methodology is presently underdeveloped. Nuclear transfer (cloning) offers an alternative approach to germline genetic modification, however there are major health concerns associated with current nuclear transfer methods. It is concluded that human germline gene therapy remains for all practical purposes a future possibility that must await significant and important advances in gene transfer technology.
Original language | English |
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Pages (from-to) | 247-268 |
Number of pages | 22 |
Journal | International Journal of Medical Sciences |
Volume | 34 |
Issue number | 4 |
DOIs | |
Publication status | Published - Jun 2004 |
Keywords
- Sperm-mediated gene therapy
- Gene transfer
- Genetic modification
- Pronuclear microinjection
- Gene targeting
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Dive into the research topics of 'Gene therapy: the potential applicability of gene transfer technology to the human germline'. Together they form a unique fingerprint.Student Theses
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The prospects for human germline genetic modification: scientific and bioethical considerations
Author: Smith, K., Dec 2004Student thesis: Doctoral Thesis
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