Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 247-268 |
| Number of pages | 22 |
| Journal | International Journal of Medical Sciences |
| Volume | 34 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jun 2004 |
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Gene therapy : the potential applicability of gene transfer technology to the human germline. / Smith, Kevin R.
In: International Journal of Medical Sciences, Vol. 34, No. 4, 06.2004, p. 247-268.Research output: Contribution to journal › Article
TY - JOUR
T1 - Gene therapy
T2 - International Journal of Medical Sciences
AU - Smith,Kevin R.
PY - 2004/6
Y1 - 2004/6
N2 - The theoretical possibility of applying gene transfer methodologies to the human germline is explored. Transgenic methods for genetically manipulating embryos may in principle be applied to humans. In particular, microinjection of retroviral vector appears to hold the greatest promise, with transgenic primates already obtained from this approach. Sperm-mediated gene transfer offers potentially the easiest route to the human germline, however the requisite methodology is presently underdeveloped. Nuclear transfer (cloning) offers an alternative approach to germline genetic modification, however there are major health concerns associated with current nuclear transfer methods. It is concluded that human germline gene therapy remains for all practical purposes a future possibility that must await significant and important advances in gene transfer technology.
AB - The theoretical possibility of applying gene transfer methodologies to the human germline is explored. Transgenic methods for genetically manipulating embryos may in principle be applied to humans. In particular, microinjection of retroviral vector appears to hold the greatest promise, with transgenic primates already obtained from this approach. Sperm-mediated gene transfer offers potentially the easiest route to the human germline, however the requisite methodology is presently underdeveloped. Nuclear transfer (cloning) offers an alternative approach to germline genetic modification, however there are major health concerns associated with current nuclear transfer methods. It is concluded that human germline gene therapy remains for all practical purposes a future possibility that must await significant and important advances in gene transfer technology.
U2 - 10.7150/ijms.1.76
DO - 10.7150/ijms.1.76
M3 - Article
VL - 34
SP - 247
EP - 268
JO - International Journal of Medical Sciences
JF - International Journal of Medical Sciences
SN - 1449-1907
IS - 4
ER -