This study investigated the relationship between handedness, gender and behavioural approach and inhibition using Carver and White’s (1994) BIS/BAS Scale. 112 participants took part: 46 left-handers and 66 right-handers. All participants completed Peters’ (1998) handedness questionnaire followed by the self-report BIS/BAS Scale. Significant effects of both handedness and gender on the BIS scores were found, with left-handers and females scoring significantly higher on inhibition. BIS scores were re-examined to include FFFS scores, which showed a significant effect of gender. Revised BIS scores replicated the original BIS findings. These findings are discussed in relation to handedness research.