Honey authenticity: evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening

S Karabournioti, G Koulis , E Panagopoulou , Fotios Stergiou, Alberto Fiore, P Katsianou, C Proestos , Nikolaos Thomaidis

    Research output: Contribution to conferenceAbstract

    Abstract

    Honey is a natural sweetener and as such it can be greatly affected by fraudulent practices like mislabeling (blossom honey sold as honeydew honey) or the addition of illegal substances (syrup or water). Phenolic compounds, flavonoids and phenolic acids, are the most abundant antioxidant nutritive constituents and proved to be beneficial to human health. The concentration and the presence of these compounds are strictly connected to the geographical and/or the botanical origin of the honey [1]. Thus, the determination of phenolic profile is of great importance for the assessment of honey authenticity. To this end, the determination of phenolic compounds was performed using an already in-house developed and validated UPLC-ESI-QTOF MS method in order to find unique marker compounds for discrimination of honey samples.
    The method was applied to over 200 unifloral and polyfloral Greek honey samples derived from 5 different botanical origins. A database consisting of 25 phenolic compounds, encountered in honey, was utilized in order to identify and quantify each of these compounds in all samples. The target screening approach was performed using Bruker TASQ 1.4 software. The identification criteria were mass accuracy, retention time, isotopic fitting as well as MS/MS fragments. The quantification involved the correction of compound signal using internal standard for compensating process losses and calculation of the concentration using standard addition curves. The selection of the most suitable internal standard for each compound was conducted prior to analysis comparing how each candidate affect the standard addition curve as well as the RSDr%. It has to be noticed that potential authenticity markers have been spotted via the target screening approach. Notably, high chrysin concentration has been measured in blossom honeys comparing to other botanical types, while quercetin concentration seems to differentiate blossom from thyme honey samples.

    KEYWORDS: honey, honey authenticity, phenolic compounds, high resolution mass spectrometry (HRMS), target screening approach

    REFERENCES:
    [1] M Camina, Jose, Roberto G Pellerano, and Eduardo J Marchevsky, Geographical and botanical classification of honeys and apicultural products by chemometric methods. A review. , Current Analytical Chemistry 8.3, pp. 408-425. 2012.

    Original languageEnglish
    Publication statusPublished - 23 Jul 2018
    Event11th Aegean Analytical Chemistry Days - International Conference Center of the Mediterranean Agronomic Institute of Chania (MAICh), Chania, Greece
    Duration: 25 Sep 201829 Sep 2018
    Conference number: 11
    http://195.134.76.37/AACD2018/AACD01_homepage.htm

    Conference

    Conference11th Aegean Analytical Chemistry Days
    Abbreviated titleAACD 2018
    CountryGreece
    CityChania
    Period25/09/1829/09/18
    Internet address

    Fingerprint

    Mass spectrometry
    Screening
    Sweetening Agents
    Quercetin
    Flavonoids
    Antioxidants
    Health
    Water
    Chemical analysis

    Cite this

    Karabournioti, S., Koulis , G., Panagopoulou , E., Stergiou, F., Fiore, A., Katsianou, P., ... Thomaidis , N. (2018). Honey authenticity: evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening. Abstract from 11th Aegean Analytical Chemistry Days, Chania, Greece.
    Karabournioti, S ; Koulis , G ; Panagopoulou , E ; Stergiou, Fotios ; Fiore, Alberto ; Katsianou, P ; Proestos , C ; Thomaidis , Nikolaos. / Honey authenticity : evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening. Abstract from 11th Aegean Analytical Chemistry Days, Chania, Greece.
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    abstract = "Honey is a natural sweetener and as such it can be greatly affected by fraudulent practices like mislabeling (blossom honey sold as honeydew honey) or the addition of illegal substances (syrup or water). Phenolic compounds, flavonoids and phenolic acids, are the most abundant antioxidant nutritive constituents and proved to be beneficial to human health. The concentration and the presence of these compounds are strictly connected to the geographical and/or the botanical origin of the honey [1]. Thus, the determination of phenolic profile is of great importance for the assessment of honey authenticity. To this end, the determination of phenolic compounds was performed using an already in-house developed and validated UPLC-ESI-QTOF MS method in order to find unique marker compounds for discrimination of honey samples. The method was applied to over 200 unifloral and polyfloral Greek honey samples derived from 5 different botanical origins. A database consisting of 25 phenolic compounds, encountered in honey, was utilized in order to identify and quantify each of these compounds in all samples. The target screening approach was performed using Bruker TASQ 1.4 software. The identification criteria were mass accuracy, retention time, isotopic fitting as well as MS/MS fragments. The quantification involved the correction of compound signal using internal standard for compensating process losses and calculation of the concentration using standard addition curves. The selection of the most suitable internal standard for each compound was conducted prior to analysis comparing how each candidate affect the standard addition curve as well as the RSDr{\%}. It has to be noticed that potential authenticity markers have been spotted via the target screening approach. Notably, high chrysin concentration has been measured in blossom honeys comparing to other botanical types, while quercetin concentration seems to differentiate blossom from thyme honey samples. KEYWORDS: honey, honey authenticity, phenolic compounds, high resolution mass spectrometry (HRMS), target screening approachREFERENCES: [1] M Camina, Jose, Roberto G Pellerano, and Eduardo J Marchevsky, Geographical and botanical classification of honeys and apicultural products by chemometric methods. A review. , Current Analytical Chemistry 8.3, pp. 408-425. 2012.",
    author = "S Karabournioti and G Koulis and E Panagopoulou and Fotios Stergiou and Alberto Fiore and P Katsianou and C Proestos and Nikolaos Thomaidis",
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    day = "23",
    language = "English",
    note = "11th Aegean Analytical Chemistry Days, AACD 2018 ; Conference date: 25-09-2018 Through 29-09-2018",
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    Karabournioti, S, Koulis , G, Panagopoulou , E, Stergiou, F, Fiore, A, Katsianou, P, Proestos , C & Thomaidis , N 2018, 'Honey authenticity: evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening', 11th Aegean Analytical Chemistry Days, Chania, Greece, 25/09/18 - 29/09/18.

    Honey authenticity : evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening. / Karabournioti, S; Koulis , G; Panagopoulou , E; Stergiou, Fotios; Fiore, Alberto; Katsianou, P; Proestos , C; Thomaidis , Nikolaos.

    2018. Abstract from 11th Aegean Analytical Chemistry Days, Chania, Greece.

    Research output: Contribution to conferenceAbstract

    TY - CONF

    T1 - Honey authenticity

    T2 - evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening

    AU - Karabournioti, S

    AU - Koulis , G

    AU - Panagopoulou , E

    AU - Stergiou, Fotios

    AU - Fiore, Alberto

    AU - Katsianou, P

    AU - Proestos , C

    AU - Thomaidis , Nikolaos

    PY - 2018/7/23

    Y1 - 2018/7/23

    N2 - Honey is a natural sweetener and as such it can be greatly affected by fraudulent practices like mislabeling (blossom honey sold as honeydew honey) or the addition of illegal substances (syrup or water). Phenolic compounds, flavonoids and phenolic acids, are the most abundant antioxidant nutritive constituents and proved to be beneficial to human health. The concentration and the presence of these compounds are strictly connected to the geographical and/or the botanical origin of the honey [1]. Thus, the determination of phenolic profile is of great importance for the assessment of honey authenticity. To this end, the determination of phenolic compounds was performed using an already in-house developed and validated UPLC-ESI-QTOF MS method in order to find unique marker compounds for discrimination of honey samples. The method was applied to over 200 unifloral and polyfloral Greek honey samples derived from 5 different botanical origins. A database consisting of 25 phenolic compounds, encountered in honey, was utilized in order to identify and quantify each of these compounds in all samples. The target screening approach was performed using Bruker TASQ 1.4 software. The identification criteria were mass accuracy, retention time, isotopic fitting as well as MS/MS fragments. The quantification involved the correction of compound signal using internal standard for compensating process losses and calculation of the concentration using standard addition curves. The selection of the most suitable internal standard for each compound was conducted prior to analysis comparing how each candidate affect the standard addition curve as well as the RSDr%. It has to be noticed that potential authenticity markers have been spotted via the target screening approach. Notably, high chrysin concentration has been measured in blossom honeys comparing to other botanical types, while quercetin concentration seems to differentiate blossom from thyme honey samples. KEYWORDS: honey, honey authenticity, phenolic compounds, high resolution mass spectrometry (HRMS), target screening approachREFERENCES: [1] M Camina, Jose, Roberto G Pellerano, and Eduardo J Marchevsky, Geographical and botanical classification of honeys and apicultural products by chemometric methods. A review. , Current Analytical Chemistry 8.3, pp. 408-425. 2012.

    AB - Honey is a natural sweetener and as such it can be greatly affected by fraudulent practices like mislabeling (blossom honey sold as honeydew honey) or the addition of illegal substances (syrup or water). Phenolic compounds, flavonoids and phenolic acids, are the most abundant antioxidant nutritive constituents and proved to be beneficial to human health. The concentration and the presence of these compounds are strictly connected to the geographical and/or the botanical origin of the honey [1]. Thus, the determination of phenolic profile is of great importance for the assessment of honey authenticity. To this end, the determination of phenolic compounds was performed using an already in-house developed and validated UPLC-ESI-QTOF MS method in order to find unique marker compounds for discrimination of honey samples. The method was applied to over 200 unifloral and polyfloral Greek honey samples derived from 5 different botanical origins. A database consisting of 25 phenolic compounds, encountered in honey, was utilized in order to identify and quantify each of these compounds in all samples. The target screening approach was performed using Bruker TASQ 1.4 software. The identification criteria were mass accuracy, retention time, isotopic fitting as well as MS/MS fragments. The quantification involved the correction of compound signal using internal standard for compensating process losses and calculation of the concentration using standard addition curves. The selection of the most suitable internal standard for each compound was conducted prior to analysis comparing how each candidate affect the standard addition curve as well as the RSDr%. It has to be noticed that potential authenticity markers have been spotted via the target screening approach. Notably, high chrysin concentration has been measured in blossom honeys comparing to other botanical types, while quercetin concentration seems to differentiate blossom from thyme honey samples. KEYWORDS: honey, honey authenticity, phenolic compounds, high resolution mass spectrometry (HRMS), target screening approachREFERENCES: [1] M Camina, Jose, Roberto G Pellerano, and Eduardo J Marchevsky, Geographical and botanical classification of honeys and apicultural products by chemometric methods. A review. , Current Analytical Chemistry 8.3, pp. 408-425. 2012.

    M3 - Abstract

    ER -

    Karabournioti S, Koulis G, Panagopoulou E, Stergiou F, Fiore A, Katsianou P et al. Honey authenticity: evaluation of phenolic compounds as potential authenticity markers using target LC-HRMS screening. 2018. Abstract from 11th Aegean Analytical Chemistry Days, Chania, Greece.