Human bone collagen synthesis is a rapid, nutritionally modulated process

John A. Babraj, Kenneth Smith, Daniel J.R. Cuthbertson, Peter Rickhuss, James S. Dorling, Michael J. Rennie

Research output: Contribution to journalArticle

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Abstract

We developed a direct assay of human bone collagen synthesis using [13C] or [15N] proline and applied it to determine the effects of feeding in young healthy men. Surprisingly, postabsorptive bone collagen synthesis is not sluggish, being ∼0.07%/h more rapid than that of muscle protein, and capable of being stimulated within 4 h of intravenous feeding by 66 ± 13%.

Introduction: All current methods for estimation of bone collagen turnover are indirect, depending on the assay of collagen "markers." Our aim was to develop a direct method for human bone collagen synthesis to be used to study its physiology and pathology, and specifically, in the first instance, the effect of feeding.

Materials and methods: We applied, over 2 h, flooding doses of [13C] and [15N] proline to label iliac crest bone collagen in eight young healthy men. The rate of collagen synthesis was determined as the rate of labeling of collagen hydroxyproline (assayed by gas chromatography-combustion-isotope ratio mass spectrometry in collagen extracted by differential solubility) compared with plasma proline labeling (assayed by gas chromatography-mass spectrometry). We also determined (in a second group of eight young healthy men) the effect of intravenous nutrition (glucose, lipid emulsion, and amino acids (in the ratio of 55%:30%:15% energy, respectively).

Results: Free bone proline labeling was 92 ± 6% of that of plasma proline, supporting the flooding dose assumption. Human iliac crest bone collagen is heterogeneous, with NaCl-EDTA, 0.5 M acetic acid, pepsin-acetic acid, and hot water-extractable pools being responsible for approximately 1%, 3%, 8%, and 81% of content, respectively. The synthetic rates were 0.58 ± 0.1, 0.24 ± 0.05, 0.07 ± 0.02, and 0.06 ± 0.01%/h, respectively, giving an average rate of ∼0.066%/h. [13C] and [15N] proline gave identical results. Intravenous nutrition caused the disappearance of proline label from the procollagen pool and its increased appearance in the less extractable pools, suggesting nutritional stimulation of collagen processing.

Conclusion: The results show (1) that iliac crest bone collagen synthesis is faster than generally assumed and of the same order as muscle protein turnover and (2) that feeding increases synthesis by approximately 66%. Given its ability to detect physiologically meaningful responses, the method should provide a new approach to studying the regulation of bone collagen turnover.
Original languageEnglish
Pages (from-to)930-937
Number of pages8
JournalJournal of Bone and Mineral Research
Volume20
Issue number6
Early online date14 Feb 2005
DOIs
Publication statusPublished - Jun 2005
Externally publishedYes

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Collagen
Bone and Bones
Proline
Muscle Proteins
Bone Remodeling
Acetic Acid
Procollagen
Pepsin A
Hydroxyproline
Parenteral Nutrition
Emulsions
Edetic Acid
Isotopes
Gas Chromatography
Solubility
Gas Chromatography-Mass Spectrometry
Mass Spectrometry
Pathology
Lipids
Amino Acids

Cite this

Babraj, J. A., Smith, K., Cuthbertson, D. J. R., Rickhuss, P., Dorling, J. S., & Rennie, M. J. (2005). Human bone collagen synthesis is a rapid, nutritionally modulated process. Journal of Bone and Mineral Research, 20(6), 930-937. https://doi.org/10.1359/JBMR.050201
Babraj, John A. ; Smith, Kenneth ; Cuthbertson, Daniel J.R. ; Rickhuss, Peter ; Dorling, James S. ; Rennie, Michael J. / Human bone collagen synthesis is a rapid, nutritionally modulated process. In: Journal of Bone and Mineral Research. 2005 ; Vol. 20, No. 6. pp. 930-937.
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Babraj, JA, Smith, K, Cuthbertson, DJR, Rickhuss, P, Dorling, JS & Rennie, MJ 2005, 'Human bone collagen synthesis is a rapid, nutritionally modulated process', Journal of Bone and Mineral Research, vol. 20, no. 6, pp. 930-937. https://doi.org/10.1359/JBMR.050201

Human bone collagen synthesis is a rapid, nutritionally modulated process. / Babraj, John A.; Smith, Kenneth; Cuthbertson, Daniel J.R.; Rickhuss, Peter; Dorling, James S.; Rennie, Michael J.

In: Journal of Bone and Mineral Research, Vol. 20, No. 6, 06.2005, p. 930-937.

Research output: Contribution to journalArticle

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T1 - Human bone collagen synthesis is a rapid, nutritionally modulated process

AU - Babraj, John A.

AU - Smith, Kenneth

AU - Cuthbertson, Daniel J.R.

AU - Rickhuss, Peter

AU - Dorling, James S.

AU - Rennie, Michael J.

PY - 2005/6

Y1 - 2005/6

N2 - We developed a direct assay of human bone collagen synthesis using [13C] or [15N] proline and applied it to determine the effects of feeding in young healthy men. Surprisingly, postabsorptive bone collagen synthesis is not sluggish, being ∼0.07%/h more rapid than that of muscle protein, and capable of being stimulated within 4 h of intravenous feeding by 66 ± 13%.Introduction: All current methods for estimation of bone collagen turnover are indirect, depending on the assay of collagen "markers." Our aim was to develop a direct method for human bone collagen synthesis to be used to study its physiology and pathology, and specifically, in the first instance, the effect of feeding.Materials and methods: We applied, over 2 h, flooding doses of [13C] and [15N] proline to label iliac crest bone collagen in eight young healthy men. The rate of collagen synthesis was determined as the rate of labeling of collagen hydroxyproline (assayed by gas chromatography-combustion-isotope ratio mass spectrometry in collagen extracted by differential solubility) compared with plasma proline labeling (assayed by gas chromatography-mass spectrometry). We also determined (in a second group of eight young healthy men) the effect of intravenous nutrition (glucose, lipid emulsion, and amino acids (in the ratio of 55%:30%:15% energy, respectively).Results: Free bone proline labeling was 92 ± 6% of that of plasma proline, supporting the flooding dose assumption. Human iliac crest bone collagen is heterogeneous, with NaCl-EDTA, 0.5 M acetic acid, pepsin-acetic acid, and hot water-extractable pools being responsible for approximately 1%, 3%, 8%, and 81% of content, respectively. The synthetic rates were 0.58 ± 0.1, 0.24 ± 0.05, 0.07 ± 0.02, and 0.06 ± 0.01%/h, respectively, giving an average rate of ∼0.066%/h. [13C] and [15N] proline gave identical results. Intravenous nutrition caused the disappearance of proline label from the procollagen pool and its increased appearance in the less extractable pools, suggesting nutritional stimulation of collagen processing.Conclusion: The results show (1) that iliac crest bone collagen synthesis is faster than generally assumed and of the same order as muscle protein turnover and (2) that feeding increases synthesis by approximately 66%. Given its ability to detect physiologically meaningful responses, the method should provide a new approach to studying the regulation of bone collagen turnover.

AB - We developed a direct assay of human bone collagen synthesis using [13C] or [15N] proline and applied it to determine the effects of feeding in young healthy men. Surprisingly, postabsorptive bone collagen synthesis is not sluggish, being ∼0.07%/h more rapid than that of muscle protein, and capable of being stimulated within 4 h of intravenous feeding by 66 ± 13%.Introduction: All current methods for estimation of bone collagen turnover are indirect, depending on the assay of collagen "markers." Our aim was to develop a direct method for human bone collagen synthesis to be used to study its physiology and pathology, and specifically, in the first instance, the effect of feeding.Materials and methods: We applied, over 2 h, flooding doses of [13C] and [15N] proline to label iliac crest bone collagen in eight young healthy men. The rate of collagen synthesis was determined as the rate of labeling of collagen hydroxyproline (assayed by gas chromatography-combustion-isotope ratio mass spectrometry in collagen extracted by differential solubility) compared with plasma proline labeling (assayed by gas chromatography-mass spectrometry). We also determined (in a second group of eight young healthy men) the effect of intravenous nutrition (glucose, lipid emulsion, and amino acids (in the ratio of 55%:30%:15% energy, respectively).Results: Free bone proline labeling was 92 ± 6% of that of plasma proline, supporting the flooding dose assumption. Human iliac crest bone collagen is heterogeneous, with NaCl-EDTA, 0.5 M acetic acid, pepsin-acetic acid, and hot water-extractable pools being responsible for approximately 1%, 3%, 8%, and 81% of content, respectively. The synthetic rates were 0.58 ± 0.1, 0.24 ± 0.05, 0.07 ± 0.02, and 0.06 ± 0.01%/h, respectively, giving an average rate of ∼0.066%/h. [13C] and [15N] proline gave identical results. Intravenous nutrition caused the disappearance of proline label from the procollagen pool and its increased appearance in the less extractable pools, suggesting nutritional stimulation of collagen processing.Conclusion: The results show (1) that iliac crest bone collagen synthesis is faster than generally assumed and of the same order as muscle protein turnover and (2) that feeding increases synthesis by approximately 66%. Given its ability to detect physiologically meaningful responses, the method should provide a new approach to studying the regulation of bone collagen turnover.

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JO - Journal of Bone and Mineral Research

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SN - 0884-0431

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