Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6

Douglas H. Lester, Colin Farquharson, George Russell, Brian Houston

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Ubiquitin-conjugating enzymes (UBCs) selectively target proteins for proteasomal degradation by the covalent attachment of ubiquitin moieties. Yeast UBC6 is unusual in having an active site distinct from all other UBCs and in possessing a transmembrane domain that anchors it to the cytoplasmic surface of the endoplasmic reticulum. During a differential display analysis on chick growth plate chondrocytes we isolated a cDNA encoding a noncanonical ubiquitin-conjugating enzyme (NCUBE1) structurally similar to yeast UBC6. Chick NCUBE1 transcripts were detected in all tissues examined and decreased threefold during chondrocyte terminal differentiation. Database searches identified other related proteins; the human and mouse orthologues of NCUBE1, a second human homologue of yeast UBC6 (NCUBE2), and related proteins from S. pombe, C. elegans, and P. mariana. Together with yeast UBC6 these proteins constitute a distinct family of UBCs sharing a conserved noncanonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6 they are likely to be localised to the endoplasmic reticulum where they may be involved in targeting retrotranslocated, ER-associated proteins for proteasomal degradation.
Original languageEnglish
Pages (from-to)474-480
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume269
Issue number2
DOIs
Publication statusPublished - 16 Mar 2000

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Ubiquitin-Conjugating Enzymes
Yeast
Yeasts
Chondrocytes
Endoplasmic Reticulum
Proteolysis
Catalytic Domain
Schizosaccharomyces pombe Proteins
Proteins
Fungal Proteins
Growth Plate
Ubiquitin
Degradation
Anchors
Complementary DNA
Databases
Display devices
Tissue

Cite this

Lester, Douglas H. ; Farquharson, Colin ; Russell, George ; Houston, Brian. / Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 269, No. 2. pp. 474-480.
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Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. / Lester, Douglas H.; Farquharson, Colin; Russell, George; Houston, Brian.

In: Biochemical and Biophysical Research Communications, Vol. 269, No. 2, 16.03.2000, p. 474-480.

Research output: Contribution to journalArticle

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