Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 477-486 |
| Number of pages | 10 |
| Journal | Yeast |
| Volume | 5 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 1989 |
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Induction of a heat shock-type response in fission yeast following nitrogen starvation. / Walker, Graeme M.; McWilliams, Philip G.
In: Yeast, Vol. 5, No. 6, 11.1989, p. 477-486.Research output: Contribution to journal › Article
TY - JOUR
T1 - Induction of a heat shock-type response in fission yeast following nitrogen starvation
AU - Walker,Graeme M.
AU - McWilliams,Philip G
PY - 1989/11
Y1 - 1989/11
N2 - When cells of the fission yeast, Schizosaccharomyces pombe, are incubated in medium devoid of a nitrogen source, they accelerate into cell division and differentially synthesize two polypeptides at 46 and 27 kD (named p46 and p27) after a delay of about an hour. The synthesis of p46 and p27 is transient. These proteins have no obvious cell cycle connection since they are also evident in nitrogen-starved (but not accelerated) cells of the temperature-sensitive mutant of S. pombe, wee 1·50h−. We infer from this that p46 and p27 are synthesized as a direct result of nutritional stress. The possibility that p46 and p27 represent examples of general environmental stress proteins was investigated by comparing nitrogen starvation with the heat-shock response in S. pombe. Heat-shock analysis of cells revealed the existence of two proteins of similar Mr to p46 and p27. In addition, nitrogen-starved cells acquired thermotolerance in a manner similar to heat-shocking cells. We suggest that nitrogen starvation in fission yeast induces a subset of the total array of heat-shock proteins.
AB - When cells of the fission yeast, Schizosaccharomyces pombe, are incubated in medium devoid of a nitrogen source, they accelerate into cell division and differentially synthesize two polypeptides at 46 and 27 kD (named p46 and p27) after a delay of about an hour. The synthesis of p46 and p27 is transient. These proteins have no obvious cell cycle connection since they are also evident in nitrogen-starved (but not accelerated) cells of the temperature-sensitive mutant of S. pombe, wee 1·50h−. We infer from this that p46 and p27 are synthesized as a direct result of nutritional stress. The possibility that p46 and p27 represent examples of general environmental stress proteins was investigated by comparing nitrogen starvation with the heat-shock response in S. pombe. Heat-shock analysis of cells revealed the existence of two proteins of similar Mr to p46 and p27. In addition, nitrogen-starved cells acquired thermotolerance in a manner similar to heat-shocking cells. We suggest that nitrogen starvation in fission yeast induces a subset of the total array of heat-shock proteins.
U2 - 10.1002/yea.320050607
DO - 10.1002/yea.320050607
M3 - Article
VL - 5
SP - 477
EP - 486
JO - Yeast
T2 - Yeast
JF - Yeast
SN - 0749-503X
IS - 6
ER -