Induction of a heat shock-type response in fission yeast following nitrogen starvation

Graeme M. Walker, Philip G McWilliams

Research output: Contribution to journalArticle

Abstract

When cells of the fission yeast, Schizosaccharomyces pombe, are incubated in medium devoid of a nitrogen source, they accelerate into cell division and differentially synthesize two polypeptides at 46 and 27 kD (named p46 and p27) after a delay of about an hour. The synthesis of p46 and p27 is transient. These proteins have no obvious cell cycle connection since they are also evident in nitrogen-starved (but not accelerated) cells of the temperature-sensitive mutant of S. pombe, wee 1·50h−. We infer from this that p46 and p27 are synthesized as a direct result of nutritional stress. The possibility that p46 and p27 represent examples of general environmental stress proteins was investigated by comparing nitrogen starvation with the heat-shock response in S. pombe. Heat-shock analysis of cells revealed the existence of two proteins of similar Mr to p46 and p27. In addition, nitrogen-starved cells acquired thermotolerance in a manner similar to heat-shocking cells. We suggest that nitrogen starvation in fission yeast induces a subset of the total array of heat-shock proteins.
Original languageEnglish
Pages (from-to)477-486
Number of pages10
JournalYeast
Volume5
Issue number6
DOIs
StatePublished - Nov 1989

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Heat-Shock Response
Schizosaccharomyces
Starvation
Nitrogen
Proteins
Hot Temperature
Yeast
HLA Antigens
Heat-Shock Proteins
Cells
Castration
Cell Division
Cell Cycle
Peptides
Temperature
Polypeptides
Acetanilides
Addison Disease
Cerebellar Ataxia
Genetic Engineering

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Walker, Graeme M.; McWilliams, Philip G / Induction of a heat shock-type response in fission yeast following nitrogen starvation.

In: Yeast, Vol. 5, No. 6, 11.1989, p. 477-486.

Research output: Contribution to journalArticle

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Induction of a heat shock-type response in fission yeast following nitrogen starvation. / Walker, Graeme M.; McWilliams, Philip G.

In: Yeast, Vol. 5, No. 6, 11.1989, p. 477-486.

Research output: Contribution to journalArticle

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AB - When cells of the fission yeast, Schizosaccharomyces pombe, are incubated in medium devoid of a nitrogen source, they accelerate into cell division and differentially synthesize two polypeptides at 46 and 27 kD (named p46 and p27) after a delay of about an hour. The synthesis of p46 and p27 is transient. These proteins have no obvious cell cycle connection since they are also evident in nitrogen-starved (but not accelerated) cells of the temperature-sensitive mutant of S. pombe, wee 1·50h−. We infer from this that p46 and p27 are synthesized as a direct result of nutritional stress. The possibility that p46 and p27 represent examples of general environmental stress proteins was investigated by comparing nitrogen starvation with the heat-shock response in S. pombe. Heat-shock analysis of cells revealed the existence of two proteins of similar Mr to p46 and p27. In addition, nitrogen-starved cells acquired thermotolerance in a manner similar to heat-shocking cells. We suggest that nitrogen starvation in fission yeast induces a subset of the total array of heat-shock proteins.

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