Induction of acrosomal exocytosis in chicken spermatozoa by inner perivitelline-derived N-linked glycans

Janet Horrocks, Sarah Stewart, Lynn Jackson, Graham J. Wishart

    Research output: Contribution to journalArticle

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    Abstract

    In birds, the ovum is surrounded by a glycoprotein coat known as the inner perivitelline layer (IPVL), which is analogous to the mammalian zona pellucida and, as such, is the site of initial sperm binding and induction of acrosomal exocytosis (the acrosome reaction). In this study, we demonstrate that oligosaccharides isolated from chicken-IPVL glycoproteins are capable of inducing the acrosome reaction in chicken spermatozoa. Preparations containing only O-linked glycans were unable to induce the acrosome reaction whereas N-linked oligosaccharides released from the IPVL by PNGaseF treatment could induce the acrosome reaction. Addition of galactose to terminal N-acetyglucosamine residues suppressed the acrosome reaction-inducing capacity of the oligosaccharide preparation; however, this capacity could be restored by co-incubation with beta-galactosidase. This evidence suggests that the acrosome reaction-inducing factor is probably an N-linked oligosaccharide with terminal N-acetyl-glucosamine residues.

    Original languageEnglish
    Pages (from-to)84-89
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume278
    Issue number1
    DOIs
    Publication statusPublished - 11 Nov 2000

    Fingerprint

    Acrosome Reaction
    Exocytosis
    Oligosaccharides
    Polysaccharides
    Spermatozoa
    Chickens
    Glycoproteins
    Glucosamine
    Birds
    beta-Galactosidase
    Galactose
    Zona Pellucida
    Ovum

    Cite this

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    title = "Induction of acrosomal exocytosis in chicken spermatozoa by inner perivitelline-derived N-linked glycans",
    abstract = "In birds, the ovum is surrounded by a glycoprotein coat known as the inner perivitelline layer (IPVL), which is analogous to the mammalian zona pellucida and, as such, is the site of initial sperm binding and induction of acrosomal exocytosis (the acrosome reaction). In this study, we demonstrate that oligosaccharides isolated from chicken-IPVL glycoproteins are capable of inducing the acrosome reaction in chicken spermatozoa. Preparations containing only O-linked glycans were unable to induce the acrosome reaction whereas N-linked oligosaccharides released from the IPVL by PNGaseF treatment could induce the acrosome reaction. Addition of galactose to terminal N-acetyglucosamine residues suppressed the acrosome reaction-inducing capacity of the oligosaccharide preparation; however, this capacity could be restored by co-incubation with beta-galactosidase. This evidence suggests that the acrosome reaction-inducing factor is probably an N-linked oligosaccharide with terminal N-acetyl-glucosamine residues.",
    author = "Janet Horrocks and Sarah Stewart and Lynn Jackson and Wishart, {Graham J.}",
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    language = "English",
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    Induction of acrosomal exocytosis in chicken spermatozoa by inner perivitelline-derived N-linked glycans. / Horrocks, Janet; Stewart, Sarah; Jackson, Lynn; Wishart, Graham J.

    In: Biochemical and Biophysical Research Communications, Vol. 278, No. 1, 11.11.2000, p. 84-89.

    Research output: Contribution to journalArticle

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    T1 - Induction of acrosomal exocytosis in chicken spermatozoa by inner perivitelline-derived N-linked glycans

    AU - Horrocks, Janet

    AU - Stewart, Sarah

    AU - Jackson, Lynn

    AU - Wishart, Graham J.

    N1 - Copyright 2000 Academic Press.

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    Y1 - 2000/11/11

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    AB - In birds, the ovum is surrounded by a glycoprotein coat known as the inner perivitelline layer (IPVL), which is analogous to the mammalian zona pellucida and, as such, is the site of initial sperm binding and induction of acrosomal exocytosis (the acrosome reaction). In this study, we demonstrate that oligosaccharides isolated from chicken-IPVL glycoproteins are capable of inducing the acrosome reaction in chicken spermatozoa. Preparations containing only O-linked glycans were unable to induce the acrosome reaction whereas N-linked oligosaccharides released from the IPVL by PNGaseF treatment could induce the acrosome reaction. Addition of galactose to terminal N-acetyglucosamine residues suppressed the acrosome reaction-inducing capacity of the oligosaccharide preparation; however, this capacity could be restored by co-incubation with beta-galactosidase. This evidence suggests that the acrosome reaction-inducing factor is probably an N-linked oligosaccharide with terminal N-acetyl-glucosamine residues.

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