Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

Scott Cameron, Karen McLuskey, Rachel Chamberlayne, Irene Hallyburton, William N. Hunter*

*Corresponding author for this work

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 Å, γ = 120°. The Matthews coefficient, VM, of 2.7 Å3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

    Original languageEnglish
    Pages (from-to)534-536
    Number of pages3
    JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
    Volume61
    Issue number5
    DOIs
    Publication statusPublished - 2005

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    Streptomyces
    enzymes
    Fosfomycin
    Ethylene Oxide
    Crystals
    acids
    Gene encoding
    antibiotics
    Enzymes
    Prosthetics
    Biological Products
    Heme
    genes
    crystals
    Metal ions
    metal ions
    coding
    Metals
    Ions
    Anti-Bacterial Agents

    Cite this

    @article{0054a73288d74a0a86aa4ceb7475a16e,
    title = "Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis",
    abstract = "The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 {\AA}, γ = 120°. The Matthews coefficient, VM, of 2.7 {\AA}3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55{\%} solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.",
    author = "Scott Cameron and Karen McLuskey and Rachel Chamberlayne and Irene Hallyburton and Hunter, {William N.}",
    year = "2005",
    doi = "10.1107/S1744309105012376",
    language = "English",
    volume = "61",
    pages = "534--536",
    journal = "Acta Crystallographica Section F:Structural Biology Communications",
    issn = "1744-3091",
    publisher = "John Wiley and Sons Ltd",
    number = "5",

    }

    Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis. / Cameron, Scott; McLuskey, Karen; Chamberlayne, Rachel; Hallyburton, Irene; Hunter, William N.

    In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 5, 2005, p. 534-536.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

    AU - Cameron, Scott

    AU - McLuskey, Karen

    AU - Chamberlayne, Rachel

    AU - Hallyburton, Irene

    AU - Hunter, William N.

    PY - 2005

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    N2 - The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 Å, γ = 120°. The Matthews coefficient, VM, of 2.7 Å3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

    AB - The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 Å, γ = 120°. The Matthews coefficient, VM, of 2.7 Å3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

    U2 - 10.1107/S1744309105012376

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    EP - 536

    JO - Acta Crystallographica Section F:Structural Biology Communications

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