Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

Scott Cameron; Karen McLuskey; Rachel Chamberlayne; Irene Hallyburton; William N. Hunter

doi:10.1107/S1744309105012376

Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

Scott Cameron, Karen McLuskey, Rachel Chamberlayne, Irene Hallyburton, William N. Hunter^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P6_1/522 and unit-cell parameters a = 86.44, c = 221.56 Å, γ = 120°. The Matthews coefficient, V_M, of 2.7 Å³ Da^-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

Original language	English
Pages (from-to)	534-536
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	5
DOIs	https://doi.org/10.1107/S1744309105012376
Publication status	Published - 2005

Keywords

Antibiotics
Epoxidases
Metalloenzymes
Streptomyces

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10.1107/S1744309105012376

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@article{0054a73288d74a0a86aa4ceb7475a16e,

title = "Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis",

abstract = "The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 {\AA}, γ = 120°. The Matthews coefficient, VM, of 2.7 {\AA}3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.",

author = "Scott Cameron and Karen McLuskey and Rachel Chamberlayne and Irene Hallyburton and Hunter, {William N.}",

year = "2005",

doi = "10.1107/S1744309105012376",

language = "English",

volume = "61",

pages = "534--536",

journal = "Acta Crystallographica Section F:Structural Biology Communications",

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publisher = "John Wiley and Sons Ltd",

number = "5",

}

Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis. / Cameron, Scott; McLuskey, Karen; Chamberlayne, Rachel; Hallyburton, Irene; Hunter, William N.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 5, 2005, p. 534-536.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

AU - Cameron, Scott

AU - McLuskey, Karen

AU - Chamberlayne, Rachel

AU - Hallyburton, Irene

AU - Hunter, William N.

PY - 2005

Y1 - 2005

N2 - The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 Å, γ = 120°. The Matthews coefficient, VM, of 2.7 Å3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

AB - The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P61/522 and unit-cell parameters a = 86.44, c = 221.56 Å, γ = 120°. The Matthews coefficient, VM, of 2.7 Å3 Da-1 corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

U2 - 10.1107/S1744309105012376

DO - 10.1107/S1744309105012376

M3 - Article

C2 - 16511089

AN - SCOPUS:26444479753

VL - 61

SP - 534

EP - 536

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 5

ER -

Initiating a crystallographic analysis of recombinant (S)-2- hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

Abstract

Keywords

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