Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione: phenotypic changes suggest the involvement of efflux pumps

Suzanne M. Abdel Malek, I. S. I. Al-Adham, Khalid Z. Matalka, Phillip J. Collier

Research output: Contribution to journalArticle

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Abstract

The aim of this study is to investigate the involvement of an efflux pump in the development of Pseudomonas aeruginosa resistance to zinc pyrithione (ZnPT). In the presence of efflux inhibitor carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the minimum inhibitory concentration of ZnPT for P. aeruginosa resistant cells is reduced significantly (p < 0.05). In addition, the concentration of ZnPT excluded by the resistant bacteria was reduced significantly (p < 0.01). However, the above reductions did not reach the levels measured for P. aeruginosa PAO1 sensitive strain. Furthermore, such changes in P. aeruginosa resistant cells were correlated with the overexpression of outer membrane proteins, reduced sensitivity toward imipenem (p < 0.01) and increased sensitivity toward sulphatriad and chloramphenicol (p < 0.05). In a continuation to a previous study, we conclude that P. aeruginosa resistance to ZnPT is multifactorial and involves induced efflux systems.


Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalCurrent Microbiology
Volume59
Issue number2
DOIs
StatePublished - Aug 2009

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Pseudomonas aeruginosa
Zinc
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Hydrazones
Imipenem
Cyanides
Malus
Microbial Sensitivity Tests
Chloramphenicol
Membrane Proteins
Bacteria

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Abdel Malek, Suzanne M.; Al-Adham, I. S. I.; Matalka, Khalid Z.; Collier, Phillip J. / Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione : phenotypic changes suggest the involvement of efflux pumps.

In: Current Microbiology, Vol. 59, No. 2, 08.2009, p. 95-100.

Research output: Contribution to journalArticle

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title = "Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione: phenotypic changes suggest the involvement of efflux pumps",
abstract = "The aim of this study is to investigate the involvement of an efflux pump in the development of Pseudomonas aeruginosa resistance to zinc pyrithione (ZnPT). In the presence of efflux inhibitor carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the minimum inhibitory concentration of ZnPT for P. aeruginosa resistant cells is reduced significantly (p < 0.05). In addition, the concentration of ZnPT excluded by the resistant bacteria was reduced significantly (p < 0.01). However, the above reductions did not reach the levels measured for P. aeruginosa PAO1 sensitive strain. Furthermore, such changes in P. aeruginosa resistant cells were correlated with the overexpression of outer membrane proteins, reduced sensitivity toward imipenem (p < 0.01) and increased sensitivity toward sulphatriad and chloramphenicol (p < 0.05). In a continuation to a previous study, we conclude that P. aeruginosa resistance to ZnPT is multifactorial and involves induced efflux systems.",
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Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione : phenotypic changes suggest the involvement of efflux pumps. / Abdel Malek, Suzanne M.; Al-Adham, I. S. I.; Matalka, Khalid Z.; Collier, Phillip J.

In: Current Microbiology, Vol. 59, No. 2, 08.2009, p. 95-100.

Research output: Contribution to journalArticle

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AB - The aim of this study is to investigate the involvement of an efflux pump in the development of Pseudomonas aeruginosa resistance to zinc pyrithione (ZnPT). In the presence of efflux inhibitor carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the minimum inhibitory concentration of ZnPT for P. aeruginosa resistant cells is reduced significantly (p < 0.05). In addition, the concentration of ZnPT excluded by the resistant bacteria was reduced significantly (p < 0.01). However, the above reductions did not reach the levels measured for P. aeruginosa PAO1 sensitive strain. Furthermore, such changes in P. aeruginosa resistant cells were correlated with the overexpression of outer membrane proteins, reduced sensitivity toward imipenem (p < 0.01) and increased sensitivity toward sulphatriad and chloramphenicol (p < 0.05). In a continuation to a previous study, we conclude that P. aeruginosa resistance to ZnPT is multifactorial and involves induced efflux systems.

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