Pseudomonas aeruginosa PAO1 Resistance to Zinc Pyrithione: phenotypic changes suggest the involvement of efflux pumps

Suzanne M. Abdel Malek, I. S. I. Al-Adham, Khalid Z. Matalka, Phillip J. Collier

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    8 Citations (Scopus)

    Abstract

    The aim of this study is to investigate the involvement of an efflux pump in the development of Pseudomonas aeruginosa resistance to zinc pyrithione (ZnPT). In the presence of efflux inhibitor carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the minimum inhibitory concentration of ZnPT for P. aeruginosa resistant cells is reduced significantly (p < 0.05). In addition, the concentration of ZnPT excluded by the resistant bacteria was reduced significantly (p < 0.01). However, the above reductions did not reach the levels measured for P. aeruginosa PAO1 sensitive strain. Furthermore, such changes in P. aeruginosa resistant cells were correlated with the overexpression of outer membrane proteins, reduced sensitivity toward imipenem (p < 0.01) and increased sensitivity toward sulphatriad and chloramphenicol (p < 0.05). In a continuation to a previous study, we conclude that P. aeruginosa resistance to ZnPT is multifactorial and involves induced efflux systems.


    Original languageEnglish
    Pages (from-to)95-100
    Number of pages6
    JournalCurrent Microbiology
    Volume59
    Issue number2
    DOIs
    Publication statusPublished - Aug 2009

    Keywords

    • Pseudomonas aeruginosa

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