Pseudomonas in the underworld: the secret life of Pseudomonas fluorescens SBW25

G. Preston, A. Spiers, X.-X. Zhang, R. Jackson, M. Gal, C. Knight, S. Gehrig, J. Malone, C. Moon, S. Godfrey, Z. Robinson, N. Bertrand, D. Field, P. Rainey

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Pseudomonas fluorescens SBW25 is a gram-negative, fluorescent pseudomonad that is able to colonise the roots and leaves of a wide range of plants. PfSBW25 has been widely adopted as a model organism for investigations into the molecular basis of plant colonisation, plant growth promotion and biocontrol, and is also used as a model organism in studies of biofilm formation and experimental evolution. The 6.7 Mb genome of PfSBW25 is currently being sequenced at the Sanger Institute. We have developed two in vivo expression technology (IVET) systems for P. fluorescens SBW25, and have used IVET as a functional genomics approach to identify and characterise more than a hundred plant-induced genes from P. fluorescens SBW25. The predicted functions of many of these genes can be assigned to broad functional categories such as nutrient acquisition, surface colonisation, stress tolerance, antibiotic biosynthesis, protein secretion and transcriptional regulation. We have begun the process of analysing the function and expression of some of these plant-induced genes in depth, focusing in particular on genes encoding a rhizosphere-induced type III protein secretion system (TTSS) that is similar to the Hrp TTSS of P. syringae. Functional genomic analyses of P. fluorescens SBW25 have provided insight into the biology and ecology of plant-colonising Pseudomonas, and into the utility and limitations of functional genomic techniques in understanding the biology of bacteria in complex environments.
    Original languageEnglish
    Title of host publicationPseudomonas syringae and related pathogens
    Subtitle of host publicationbiology and genetic
    EditorsNicola Sante Iacobellis, Alan Collmer, Steven Hutcheson, John Mansfield, Cindy E. Morris, Jesús Murillo, Norman W. Schaad, David E. Stead, Giuseppe Surico, Matthias S. Ullrich
    Place of PublicationDordrecht
    PublisherKluwer Academic Publishers
    Pages347-353
    Number of pages7
    Edition1
    ISBN (Electronic)9789401701334
    ISBN (Print)9789048162673, 9781402012273
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Pseudomonas fluorescens
    Pseudomonas
    protein secretion
    genomics
    genes
    plant ecology
    growth promotion
    plant biology
    organisms
    stress tolerance
    biofilm
    rhizosphere
    biological control
    antibiotics
    plant growth
    biosynthesis
    Biological Sciences
    genome
    bacteria
    nutrients

    Cite this

    Preston, G., Spiers, A., Zhang, X-X., Jackson, R., Gal, M., Knight, C., ... Rainey, P. (2003). Pseudomonas in the underworld: the secret life of Pseudomonas fluorescens SBW25. In N. S. Iacobellis, A. Collmer, S. Hutcheson, J. Mansfield, C. E. Morris, J. Murillo, N. W. Schaad, D. E. Stead, G. Surico, ... M. S. Ullrich (Eds.), Pseudomonas syringae and related pathogens: biology and genetic (1 ed., pp. 347-353). Dordrecht: Kluwer Academic Publishers. https://doi.org/10.1007/978-94-017-0133-4_37
    Preston, G. ; Spiers, A. ; Zhang, X.-X. ; Jackson, R. ; Gal, M. ; Knight, C. ; Gehrig, S. ; Malone, J. ; Moon, C. ; Godfrey, S. ; Robinson, Z. ; Bertrand, N. ; Field, D. ; Rainey, P. / Pseudomonas in the underworld : the secret life of Pseudomonas fluorescens SBW25. Pseudomonas syringae and related pathogens: biology and genetic. editor / Nicola Sante Iacobellis ; Alan Collmer ; Steven Hutcheson ; John Mansfield ; Cindy E. Morris ; Jesús Murillo ; Norman W. Schaad ; David E. Stead ; Giuseppe Surico ; Matthias S. Ullrich. 1. ed. Dordrecht : Kluwer Academic Publishers, 2003. pp. 347-353
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    abstract = "Pseudomonas fluorescens SBW25 is a gram-negative, fluorescent pseudomonad that is able to colonise the roots and leaves of a wide range of plants. PfSBW25 has been widely adopted as a model organism for investigations into the molecular basis of plant colonisation, plant growth promotion and biocontrol, and is also used as a model organism in studies of biofilm formation and experimental evolution. The 6.7 Mb genome of PfSBW25 is currently being sequenced at the Sanger Institute. We have developed two in vivo expression technology (IVET) systems for P. fluorescens SBW25, and have used IVET as a functional genomics approach to identify and characterise more than a hundred plant-induced genes from P. fluorescens SBW25. The predicted functions of many of these genes can be assigned to broad functional categories such as nutrient acquisition, surface colonisation, stress tolerance, antibiotic biosynthesis, protein secretion and transcriptional regulation. We have begun the process of analysing the function and expression of some of these plant-induced genes in depth, focusing in particular on genes encoding a rhizosphere-induced type III protein secretion system (TTSS) that is similar to the Hrp TTSS of P. syringae. Functional genomic analyses of P. fluorescens SBW25 have provided insight into the biology and ecology of plant-colonising Pseudomonas, and into the utility and limitations of functional genomic techniques in understanding the biology of bacteria in complex environments.",
    author = "G. Preston and A. Spiers and X.-X. Zhang and R. Jackson and M. Gal and C. Knight and S. Gehrig and J. Malone and C. Moon and S. Godfrey and Z. Robinson and N. Bertrand and D. Field and P. Rainey",
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    editor = "Iacobellis, {Nicola Sante} and Alan Collmer and Steven Hutcheson and John Mansfield and Morris, {Cindy E.} and Jes{\'u}s Murillo and Schaad, {Norman W.} and Stead, {David E.} and Giuseppe Surico and Ullrich, {Matthias S.}",
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    Preston, G, Spiers, A, Zhang, X-X, Jackson, R, Gal, M, Knight, C, Gehrig, S, Malone, J, Moon, C, Godfrey, S, Robinson, Z, Bertrand, N, Field, D & Rainey, P 2003, Pseudomonas in the underworld: the secret life of Pseudomonas fluorescens SBW25. in NS Iacobellis, A Collmer, S Hutcheson, J Mansfield, CE Morris, J Murillo, NW Schaad, DE Stead, G Surico & MS Ullrich (eds), Pseudomonas syringae and related pathogens: biology and genetic. 1 edn, Kluwer Academic Publishers, Dordrecht, pp. 347-353. https://doi.org/10.1007/978-94-017-0133-4_37

    Pseudomonas in the underworld : the secret life of Pseudomonas fluorescens SBW25. / Preston, G.; Spiers, A.; Zhang, X.-X.; Jackson, R.; Gal, M.; Knight, C.; Gehrig, S.; Malone, J.; Moon, C.; Godfrey, S.; Robinson, Z.; Bertrand, N.; Field, D.; Rainey, P.

    Pseudomonas syringae and related pathogens: biology and genetic. ed. / Nicola Sante Iacobellis; Alan Collmer; Steven Hutcheson; John Mansfield; Cindy E. Morris; Jesús Murillo; Norman W. Schaad; David E. Stead; Giuseppe Surico; Matthias S. Ullrich. 1. ed. Dordrecht : Kluwer Academic Publishers, 2003. p. 347-353.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    TY - GEN

    T1 - Pseudomonas in the underworld

    T2 - the secret life of Pseudomonas fluorescens SBW25

    AU - Preston, G.

    AU - Spiers, A.

    AU - Zhang, X.-X.

    AU - Jackson, R.

    AU - Gal, M.

    AU - Knight, C.

    AU - Gehrig, S.

    AU - Malone, J.

    AU - Moon, C.

    AU - Godfrey, S.

    AU - Robinson, Z.

    AU - Bertrand, N.

    AU - Field, D.

    AU - Rainey, P.

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    N2 - Pseudomonas fluorescens SBW25 is a gram-negative, fluorescent pseudomonad that is able to colonise the roots and leaves of a wide range of plants. PfSBW25 has been widely adopted as a model organism for investigations into the molecular basis of plant colonisation, plant growth promotion and biocontrol, and is also used as a model organism in studies of biofilm formation and experimental evolution. The 6.7 Mb genome of PfSBW25 is currently being sequenced at the Sanger Institute. We have developed two in vivo expression technology (IVET) systems for P. fluorescens SBW25, and have used IVET as a functional genomics approach to identify and characterise more than a hundred plant-induced genes from P. fluorescens SBW25. The predicted functions of many of these genes can be assigned to broad functional categories such as nutrient acquisition, surface colonisation, stress tolerance, antibiotic biosynthesis, protein secretion and transcriptional regulation. We have begun the process of analysing the function and expression of some of these plant-induced genes in depth, focusing in particular on genes encoding a rhizosphere-induced type III protein secretion system (TTSS) that is similar to the Hrp TTSS of P. syringae. Functional genomic analyses of P. fluorescens SBW25 have provided insight into the biology and ecology of plant-colonising Pseudomonas, and into the utility and limitations of functional genomic techniques in understanding the biology of bacteria in complex environments.

    AB - Pseudomonas fluorescens SBW25 is a gram-negative, fluorescent pseudomonad that is able to colonise the roots and leaves of a wide range of plants. PfSBW25 has been widely adopted as a model organism for investigations into the molecular basis of plant colonisation, plant growth promotion and biocontrol, and is also used as a model organism in studies of biofilm formation and experimental evolution. The 6.7 Mb genome of PfSBW25 is currently being sequenced at the Sanger Institute. We have developed two in vivo expression technology (IVET) systems for P. fluorescens SBW25, and have used IVET as a functional genomics approach to identify and characterise more than a hundred plant-induced genes from P. fluorescens SBW25. The predicted functions of many of these genes can be assigned to broad functional categories such as nutrient acquisition, surface colonisation, stress tolerance, antibiotic biosynthesis, protein secretion and transcriptional regulation. We have begun the process of analysing the function and expression of some of these plant-induced genes in depth, focusing in particular on genes encoding a rhizosphere-induced type III protein secretion system (TTSS) that is similar to the Hrp TTSS of P. syringae. Functional genomic analyses of P. fluorescens SBW25 have provided insight into the biology and ecology of plant-colonising Pseudomonas, and into the utility and limitations of functional genomic techniques in understanding the biology of bacteria in complex environments.

    U2 - 10.1007/978-94-017-0133-4_37

    DO - 10.1007/978-94-017-0133-4_37

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    BT - Pseudomonas syringae and related pathogens

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    A2 - Collmer, Alan

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    A2 - Murillo, Jesús

    A2 - Schaad, Norman W.

    A2 - Stead, David E.

    A2 - Surico, Giuseppe

    A2 - Ullrich, Matthias S.

    PB - Kluwer Academic Publishers

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    Preston G, Spiers A, Zhang X-X, Jackson R, Gal M, Knight C et al. Pseudomonas in the underworld: the secret life of Pseudomonas fluorescens SBW25. In Iacobellis NS, Collmer A, Hutcheson S, Mansfield J, Morris CE, Murillo J, Schaad NW, Stead DE, Surico G, Ullrich MS, editors, Pseudomonas syringae and related pathogens: biology and genetic. 1 ed. Dordrecht: Kluwer Academic Publishers. 2003. p. 347-353 https://doi.org/10.1007/978-94-017-0133-4_37