Isolation of U5 snRNP particles

G. Pironcheva*, N. Zhelev, G. Russev

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

A method for the isolation of intact U5 small nuclear RNP particles from HeLa cells has been developed. The procedure includes nuclear extraction of the particles at moderate ionic strength, fractionation in agarose gels, electrophoretic transfer on DEAE cellulose paper and elution with ammonium chloride. The purified U5 snRNP particles contain U5 RNA and a set of eleven proteins. They retain their antigenicity as judged by their reaction with autoantibodies from patients with connective tissue diseases.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalCytobios
Volume56
Issue number224
Publication statusPublished - Dec 1988
Externally publishedYes

Fingerprint

U5 Small Nuclear Ribonucleoproteins
Small Nuclear Ribonucleoproteins
Ammonium Chloride
DEAE-Cellulose
Connective Tissue Diseases
HeLa Cells
Autoantibodies
Sepharose
Osmolar Concentration
ammonium chloride
autoantibodies
Gels
isolation techniques
ionic strength
connective tissues
agarose
cellulose
RNA
fractionation
ammonium

Cite this

Pironcheva, G., Zhelev, N., & Russev, G. (1988). Isolation of U5 snRNP particles. Cytobios, 56(224), 39-44.
Pironcheva, G. ; Zhelev, N. ; Russev, G. / Isolation of U5 snRNP particles. In: Cytobios. 1988 ; Vol. 56, No. 224. pp. 39-44.
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Pironcheva, G, Zhelev, N & Russev, G 1988, 'Isolation of U5 snRNP particles', Cytobios, vol. 56, no. 224, pp. 39-44.

Isolation of U5 snRNP particles. / Pironcheva, G.; Zhelev, N.; Russev, G.

In: Cytobios, Vol. 56, No. 224, 12.1988, p. 39-44.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation of U5 snRNP particles

AU - Pironcheva, G.

AU - Zhelev, N.

AU - Russev, G.

PY - 1988/12

Y1 - 1988/12

N2 - A method for the isolation of intact U5 small nuclear RNP particles from HeLa cells has been developed. The procedure includes nuclear extraction of the particles at moderate ionic strength, fractionation in agarose gels, electrophoretic transfer on DEAE cellulose paper and elution with ammonium chloride. The purified U5 snRNP particles contain U5 RNA and a set of eleven proteins. They retain their antigenicity as judged by their reaction with autoantibodies from patients with connective tissue diseases.

AB - A method for the isolation of intact U5 small nuclear RNP particles from HeLa cells has been developed. The procedure includes nuclear extraction of the particles at moderate ionic strength, fractionation in agarose gels, electrophoretic transfer on DEAE cellulose paper and elution with ammonium chloride. The purified U5 snRNP particles contain U5 RNA and a set of eleven proteins. They retain their antigenicity as judged by their reaction with autoantibodies from patients with connective tissue diseases.

M3 - Article

VL - 56

SP - 39

EP - 44

JO - Cytobios

JF - Cytobios

SN - 0011-4529

IS - 224

ER -

Pironcheva G, Zhelev N, Russev G. Isolation of U5 snRNP particles. Cytobios. 1988 Dec;56(224):39-44.