Laser light scattering applications in biotechnology

Nikolai Zhelev, S. Barudov

    Research output: Contribution to journalReview article

    14 Citations (Scopus)
    65 Downloads (Pure)

    Abstract

    Recent advancement of laser light scattering applications in biotechnology are reviewed with emphasis on their use in the biopharmaceutical industry. Light scattering methods have been used to date to characterize biomolecules in solution. They can provide information about the size and conformation of proteins and their aggregation state as well as their ability to crystallise. In addition, modern light scattering instrumentation is becoming method of choice for studying macromolecular interactions. Interactions between macromolecules such as proteins and nucleic acids mediate fundamental processes and their modulation has led to new strategies for developing therapeutics. Light scattering approaches offer significant advantage to other approaches for studying molecular interactions. Compared with other techniques, light scattering is very quick, uses minimal sample quantities, allows recovery of the sample and does nor require derivatisation.
    Original languageEnglish
    Pages (from-to)3-8
    Number of pages6
    JournalBiotechnology and Biotechnological Equipment
    Volume19
    Issue number3
    DOIs
    Publication statusPublished - Sep 2005

    Keywords

    • Light--Scattering
    • Biotechnology

    Fingerprint

    Dive into the research topics of 'Laser light scattering applications in biotechnology'. Together they form a unique fingerprint.

    Cite this