Laser light scattering applications in biotechnology

Nikolai Zhelev, S. Barudov

Research output: Contribution to journalReview article

11 Citations (Scopus)
14 Downloads (Pure)

Abstract

Recent advancement of laser light scattering applications in biotechnology are reviewed with emphasis on their use in the biopharmaceutical industry. Light scattering methods have been used to date to characterize biomolecules in solution. They can provide information about the size and conformation of proteins and their aggregation state as well as their ability to crystallise. In addition, modern light scattering instrumentation is becoming method of choice for studying macromolecular interactions. Interactions between macromolecules such as proteins and nucleic acids mediate fundamental processes and their modulation has led to new strategies for developing therapeutics. Light scattering approaches offer significant advantage to other approaches for studying molecular interactions. Compared with other techniques, light scattering is very quick, uses minimal sample quantities, allows recovery of the sample and does nor require derivatisation.
Original languageEnglish
Pages (from-to)3-8
Number of pages6
JournalBiotechnology and Biotechnological Equipment
Volume19
Issue number3
DOIs
Publication statusPublished - Sep 2005

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Biotechnology
Lasers
Light
Protein Conformation
Nucleic Acids
Industry
Proteins
Therapeutics

Cite this

Zhelev, Nikolai ; Barudov, S. / Laser light scattering applications in biotechnology. In: Biotechnology and Biotechnological Equipment. 2005 ; Vol. 19, No. 3. pp. 3-8.
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Laser light scattering applications in biotechnology. / Zhelev, Nikolai; Barudov, S.

In: Biotechnology and Biotechnological Equipment, Vol. 19, No. 3, 09.2005, p. 3-8.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Laser light scattering applications in biotechnology

AU - Zhelev, Nikolai

AU - Barudov, S.

PY - 2005/9

Y1 - 2005/9

N2 - Recent advancement of laser light scattering applications in biotechnology are reviewed with emphasis on their use in the biopharmaceutical industry. Light scattering methods have been used to date to characterize biomolecules in solution. They can provide information about the size and conformation of proteins and their aggregation state as well as their ability to crystallise. In addition, modern light scattering instrumentation is becoming method of choice for studying macromolecular interactions. Interactions between macromolecules such as proteins and nucleic acids mediate fundamental processes and their modulation has led to new strategies for developing therapeutics. Light scattering approaches offer significant advantage to other approaches for studying molecular interactions. Compared with other techniques, light scattering is very quick, uses minimal sample quantities, allows recovery of the sample and does nor require derivatisation.

AB - Recent advancement of laser light scattering applications in biotechnology are reviewed with emphasis on their use in the biopharmaceutical industry. Light scattering methods have been used to date to characterize biomolecules in solution. They can provide information about the size and conformation of proteins and their aggregation state as well as their ability to crystallise. In addition, modern light scattering instrumentation is becoming method of choice for studying macromolecular interactions. Interactions between macromolecules such as proteins and nucleic acids mediate fundamental processes and their modulation has led to new strategies for developing therapeutics. Light scattering approaches offer significant advantage to other approaches for studying molecular interactions. Compared with other techniques, light scattering is very quick, uses minimal sample quantities, allows recovery of the sample and does nor require derivatisation.

U2 - 10.1080/13102818.2005.10817219

DO - 10.1080/13102818.2005.10817219

M3 - Review article

VL - 19

SP - 3

EP - 8

JO - Biotechnology and Biotechnological Equipment

JF - Biotechnology and Biotechnological Equipment

SN - 1310-2818

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