Limitations of biosurfactant strength produced by bacteria

Joanna Fechtner, Scott Cameron, Yusuf Y. Deeni, Simona M. Hapca, Kamaluddeen Kabir, Ibrahim U. Mohammed, Andrew J. Spiers

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)
256 Downloads (Pure)

Abstract

Biosurfactants which reduce the surface or interfacial tension of liquids and act as emulsifiers, foaming and dispersing agents with low toxicity, are finding increasing applications in biotechnology and driving the search for novel compounds for further exploitation. Potential biosurfactants sourced from bacteria are often selected first by qualitative assessment of surface tension using simple assays such as the drop collapse technique or by quantitative tensiometry measuring air-liquid interfacial tension of cell-free culture supernatants or purified samples, and subsequently tested for appropriate physicalchemical behaviours using a range of application-specific assays. Highly active or strong biosurfactants have been reported to reduce the surface tension of water to approximately 22 – 25 mN.m-1, and show a range of behaviours determined by the choice of conditions (temperature, pH, salt concentration, etc.) used to test particular aqueous-hydrophobic (oil) mixtures. However, recent analyses of biosurfactant strengths using a predictive statistical approach (Individual distribution identification) have shown that it is unlikely that new compounds will be identified able to significantly reduce aqueous surface tensions below 24 mN.m-1. The mechanistic basis of this limit requires an explanation of why stronger compounds are not produced by bacteria, with a limitation of self-harm to producing cells probably the most likely biophysical explanation. However, behavioural analyses using a combination of emulsion, foam stability and oil-dispersion assays indicates high chemical diversity exists amongst biosurfactants exhibiting the strongest levels of activity (24 – 28 mN.m-1), suggesting that bacteria are still likely to provide a rich source of potentially novel compounds for use in biotechnology.
Original languageEnglish
Title of host publicationBiosurfactants
Subtitle of host publicationoccurrences, applications and research
EditorsCharles R. Upton
Place of PublicationHauppauge, NY
PublisherNova Science Publishers
Chapter5
Pages125-148
Number of pages24
ISBN (Electronic)9781536107210
ISBN (Print)9781536107067
Publication statusPublished - 1 Jan 2017

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Surface tension
Bacteria
Assays
Biotechnology
Oils
Liquids
Emulsions
Toxicity
Foams
Salts
Water
Air
Temperature

Cite this

Fechtner, J., Cameron, S., Deeni, Y. Y., Hapca, S. M., Kabir, K., Mohammed, I. U., & Spiers, A. J. (2017). Limitations of biosurfactant strength produced by bacteria. In C. R. Upton (Ed.), Biosurfactants: occurrences, applications and research (pp. 125-148 ). Hauppauge, NY: Nova Science Publishers.
Fechtner, Joanna ; Cameron, Scott ; Deeni, Yusuf Y. ; Hapca, Simona M. ; Kabir, Kamaluddeen ; Mohammed, Ibrahim U. ; Spiers, Andrew J. / Limitations of biosurfactant strength produced by bacteria. Biosurfactants: occurrences, applications and research. editor / Charles R. Upton. Hauppauge, NY : Nova Science Publishers, 2017. pp. 125-148
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Fechtner, J, Cameron, S, Deeni, YY, Hapca, SM, Kabir, K, Mohammed, IU & Spiers, AJ 2017, Limitations of biosurfactant strength produced by bacteria. in CR Upton (ed.), Biosurfactants: occurrences, applications and research. Nova Science Publishers, Hauppauge, NY, pp. 125-148 .

Limitations of biosurfactant strength produced by bacteria. / Fechtner, Joanna; Cameron, Scott; Deeni, Yusuf Y.; Hapca, Simona M.; Kabir, Kamaluddeen; Mohammed, Ibrahim U.; Spiers, Andrew J.

Biosurfactants: occurrences, applications and research. ed. / Charles R. Upton. Hauppauge, NY : Nova Science Publishers, 2017. p. 125-148 .

Research output: Chapter in Book/Report/Conference proceedingChapter

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N2 - Biosurfactants which reduce the surface or interfacial tension of liquids and act as emulsifiers, foaming and dispersing agents with low toxicity, are finding increasing applications in biotechnology and driving the search for novel compounds for further exploitation. Potential biosurfactants sourced from bacteria are often selected first by qualitative assessment of surface tension using simple assays such as the drop collapse technique or by quantitative tensiometry measuring air-liquid interfacial tension of cell-free culture supernatants or purified samples, and subsequently tested for appropriate physicalchemical behaviours using a range of application-specific assays. Highly active or strong biosurfactants have been reported to reduce the surface tension of water to approximately 22 – 25 mN.m-1, and show a range of behaviours determined by the choice of conditions (temperature, pH, salt concentration, etc.) used to test particular aqueous-hydrophobic (oil) mixtures. However, recent analyses of biosurfactant strengths using a predictive statistical approach (Individual distribution identification) have shown that it is unlikely that new compounds will be identified able to significantly reduce aqueous surface tensions below 24 mN.m-1. The mechanistic basis of this limit requires an explanation of why stronger compounds are not produced by bacteria, with a limitation of self-harm to producing cells probably the most likely biophysical explanation. However, behavioural analyses using a combination of emulsion, foam stability and oil-dispersion assays indicates high chemical diversity exists amongst biosurfactants exhibiting the strongest levels of activity (24 – 28 mN.m-1), suggesting that bacteria are still likely to provide a rich source of potentially novel compounds for use in biotechnology.

AB - Biosurfactants which reduce the surface or interfacial tension of liquids and act as emulsifiers, foaming and dispersing agents with low toxicity, are finding increasing applications in biotechnology and driving the search for novel compounds for further exploitation. Potential biosurfactants sourced from bacteria are often selected first by qualitative assessment of surface tension using simple assays such as the drop collapse technique or by quantitative tensiometry measuring air-liquid interfacial tension of cell-free culture supernatants or purified samples, and subsequently tested for appropriate physicalchemical behaviours using a range of application-specific assays. Highly active or strong biosurfactants have been reported to reduce the surface tension of water to approximately 22 – 25 mN.m-1, and show a range of behaviours determined by the choice of conditions (temperature, pH, salt concentration, etc.) used to test particular aqueous-hydrophobic (oil) mixtures. However, recent analyses of biosurfactant strengths using a predictive statistical approach (Individual distribution identification) have shown that it is unlikely that new compounds will be identified able to significantly reduce aqueous surface tensions below 24 mN.m-1. The mechanistic basis of this limit requires an explanation of why stronger compounds are not produced by bacteria, with a limitation of self-harm to producing cells probably the most likely biophysical explanation. However, behavioural analyses using a combination of emulsion, foam stability and oil-dispersion assays indicates high chemical diversity exists amongst biosurfactants exhibiting the strongest levels of activity (24 – 28 mN.m-1), suggesting that bacteria are still likely to provide a rich source of potentially novel compounds for use in biotechnology.

M3 - Chapter

SN - 9781536107067

SP - 125

EP - 148

BT - Biosurfactants

A2 - Upton, Charles R.

PB - Nova Science Publishers

CY - Hauppauge, NY

ER -

Fechtner J, Cameron S, Deeni YY, Hapca SM, Kabir K, Mohammed IU et al. Limitations of biosurfactant strength produced by bacteria. In Upton CR, editor, Biosurfactants: occurrences, applications and research. Hauppauge, NY: Nova Science Publishers. 2017. p. 125-148